Casp9 (NM_001277932) Mouse Untagged Clone

CAT#: MC227532

Casp9 (untagged) - Mouse caspase 9 (Casp9), transcript variant 2



  "NM_001277932" in other vectors (1)

CNY 3,900.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (4)
Caspase 9 Rabbit monoclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms AI115399; APAF-3; AW493809; Casp; CASP-9; Caspase-9; ICE-; ICE-LAP6; Mch6
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227532 representing NM_001277932
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGACGAGGCGGACCGGCAGCTCCTGCGGCGATGCAGGGTGCGCCTAGTGAGCGAGCTGCAAGTCGCGG
AGCTCTGGGACGCTCTGCTGAGTCGAGAGCTCTTCACGCGCGACATGATCGAGGATATTCAGCAGGCAGG
ATCTGGGTCTCGGCGGGATCAGGCCAGGCAGCTGGTCACAGACCTTGAGACCCGAGGGAGGCAGGCCCTT
CCTCTCTTCATCTCCTGCTTAGAGGACACAGGCCAAGGCACCCTGGCTTCACTCTTGCAAAGCGGTCGGC
AAGCAGCCAAGCAGGATCCAGAGGCTGTTAAACCCCTAGACCACCTGGTGCCTGTGGTCCTGGGACCAAT
GGGACTCACAGCAAAGGAGCAGAGAGTAGTGAAGCTGGACCCGTCACAGCCTGCCGTGGGAAACCTCACC
CCAGTGGTGCTGGGGCCAGAAGAGCTCTGGCCTGCTCGGCTCAAGCCAGAGGTTCTCAGACCAGAAACAC
CCAGGCCCGTGGACATTGGTTCTGGCGGAGCTCATGATGTCTGTGTTCCAGGGAAGATCAGGGGACATGC
AGATATGGCATACACCCTGGATTCGGATCCCTGTGGCCACTGCCTCATCATCAACAATGTGAACTTCTGC
CCTTCCTCGGGGCTCGGCACACGCACGGGCTCCAACTTGGACCGTGACAAACTTGAGCACCGATTCCGCT
GGCTGCGCTTCATGGTGGAGGTGAAGAACGACCTGACTGCCAAGAAAATGGTCACGGCTTTGATGGAGAT
GGCACACCGGAACCACCGTGCCCTGGACTGCTTTGTGGTGGTCATCCTCTCTCATGGCTGCCAGGCCAGC
CACCTCCAGTTCCCGGGTGCTGTCTATGGGACAGATGGATGCTCCGTGTCCATTGAGAAAATTGTGAATA
TCTTCAACGGGAGCGGCTGCCCCAGCCTGGGAGGGAAGCCCAAGCTCTTCTTCATCCAGGCCTGCGGTGG
TGAGCAGAAAGACCATGGCTTTGAGGTGGCCTGCACTTCCTCTCAAGGCAGGACCTTGGACAGTGACTCT
GAGCCAGATGCTGTCCCCTATCAGGAAGGCCCAAGGCCCTTGGACCAGCTGGATGCTGTGTCAAGTTTGC
CTACCCCCAGTGACATCCTTGTGTCCTACTCCACCTTCCCAGGTTGCCAATGCTGTTTCTGCGAAAGGGA
CTTACAAGCAGATTCCTGGCTGTTTTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001277932
Insert Size 1218 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001277932.1, NP_001264861.1
RefSeq Size 3789 bp
RefSeq ORF 1218 bp
Locus ID 12371
Gene Summary This gene is part of a family of caspases, aspartate-specific cysteine proteases well studied for their involvement in immune and apoptosis signaling. This protein, the initiator caspase, is activated after cytochrome c release from mitochondria and targets downstream effectors. In mouse, deficiency of this gene can cause perinatal lethality. This protein may have a role in normal brain development. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Apr 2013]
Transcript Variant: This variant (2) lacks an alternate exon, which results in a frameshift, compared to variant 1. The resulting protein (isoform 2) has a distinct C-terminus, compared to isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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