Lox (NM_001286181) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AI893619; rrg; TSC-16; TSC-160
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227552 representing NM_001286181 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGTTTCGCCTGGGCTGTGCTCCTTCTGGGGCCACTGCAGCTTTGTCCCCTTCTCCGCTGCGCCCCGC AGACCCCGCGCGAGCCGCCCGCCGCCCCTGGTGCCTGGCGCCAGACAATCCAATGGGAGAACAACGGGCA GGTGTTCAGTCTGCTGAGCCTCGGGGCGCAGTACCAGCCTCAGCGACGCCGCGACCCCAGTGCCACTGCC CGGAGACCCGACGGCGACGCAGCCTCGCAGCCGCGCACGCCCATTCTTCTGCTGCGTGACAACCGCACCG CCTCTACCCGTGCGAGGACGCCAAGCCCGTCTGGGGTCGCCGCGGGTCGTCCCCGGCCCGCCGCCCGCCA CTGGTTCCAAGCTGGTTTCTCGCCGTCGGGGGCTCGCGATGGAGCCTCACGGCGCGCGGCGAACCGGACT GCATCGCCACAGCCTCCGCAGCTCAGTAATCTGAGGCCACCCAGCCACATAGATCGCATGGTGGGCGACG ACCCCTACAATCCCTACAAGTACTCCGACGACAACCCCTATTATAACTACTATGACACGTATGAGAGGCC CCGGCCCGGGAGCAGGAACCGACCTGGATACGGCACCGGTTACTTCCAGTACGGTCTCCCGGACCTGGTG CCCGACCCCTACTACATCCAGGCTTCCACGTACGTCCAGAAGATGTCTATGTACAACCTGAGATGCGCTG CGGAAGAAAACTGCCTGGCCAGTTCAGCATATAGGGCGGATGTCAGAGACTATGACCACAGGGTACTGCT ACGATTTCCGCAAAGAGTGAAGAACCAAGGGACATCGGACTTCTTACCAAGCCGCCCTCGGTACTCCTGG GAGTGGCACAGCTGTCACCAACATTACCACAGCATGGACGAATTCAGCCACTATGACCTGCTTGATGCCA ACACACAGAGGAGAGTGGCTGAAGGCCACAAAGCAAGCTTCTGTCTGGAGGACACGTCCTGTGACTATGG GTACCACAGGCGCTTTGCGTGCACTGCACACACACAGGGATTGAGTCCTGGATGTTATGACACCTATGCG GCAGACATAGACTGCCAGTGGATTGATATTACAGATGTACAACCTGGAAACTACATTCTAAAGGTCAGTG TAAACCCCAGCTACCTGGTGCCTGAATCAGACTACACTAACAATGTTGTACGCTGTGACATTCGCTACAC AGGACATCATGCCTATGCCTCAGGCTGCACAATTTCACCGTATTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286181
Insert Size	1236 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001286181.1, NP_001273110.1
RefSeq Size	4537 bp
RefSeq ORF	1236 bp
Locus ID	16948
UniProt ID	P28301
Gene Summary	This gene encodes a precursor protein that belongs to the lysyl oxidase family of proteins. The secreted proprotein is proteolytically processed to an active mature peptide and a propeptide. This propeptide is thought to function in tumor suppression by inhibiting the Ras signaling pathway. The active enzyme plays a role in cross-linking of collagen and elastin and is essential for development of cardiovascular and respiratory systems, and development of skin and connective tissue. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013] Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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