Trpc4 (NM_001253683) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CCE1; STRPC4; Trp4; Trrp4
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227667 representing NM_001253683 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGTTCTATTACAAAAGAAATGTCAACGCCCCCTACAGAGACCGCATCCCACTGAGGATTGTCA GAGCAGAATCTGAGCTCTCACCATCAGAGAAAGCCTACTTGAATGCTGTGGAGAAGGGGGACTATGCAAG CGTCAAGAAGTCTCTGGAGGAAGCTGAGATTTATTTTAAAATCAACATTAACTGCATCGACCCCCTGGGA AGGACCGCCCTCCTCATTGCCATTGAAAATGAGAATCTGGAGCTTATTGAACTATTGTTGAGTTTCAATG TCTATGTAGGCGATGCGCTGCTTCACGCCATCAGAAAAGAGGTGGTTGGAGCCGTGGAGCTACTGCTGAA CCACAAAAAGCCAAGTGGAGAGAAGCAGGTGCCTCCCATTCTCCTTGATAAACAGTTCTCTGAATTCACT CCGGACATCACACCCATCATCTTGGCTGCACATACAAATAATTACGAGATAATCAAACTTTTGGTTCAGA AAGGTGTCTCAGTGCCCAGACCCCACGAGGTCCGCTGTAACTGTGTTGAGTGTGTCTCCAGCTCGGATGT GGACAGCCTCAGGCATTCACGGTCCAGGCTCAACATCTACAAGGCCTTGGCCAGCCCCTCGCTCATTGCC CTGTCAAGCGAAGACCCTTTCCTTACTGCCTTTCAGTTAAGTTGGGAGCTGCAAGAACTCAGCAAGGTGG AGAACGAATTCAAGTCGGAGTATGAGGAGCTGTCTAGACAGTGCAAACAATTTGCCAAGGACCTCCTAGA TCAGACACGGAGTTCCAGAGAGCTGGAAATCATTCTTAATTACCGTGATGACAATAGTCTGATCGAAGAA CAGAGTGGAAATGATCTTGCAAGGCTAAAATTAGCCATTAAGTACCGTCAAAAAGAGTTTGTTGCTCAGC CCAACTGCCAGCAGCTGCTCGCTTCCCGCTGGTACGATGAGTTCCCAGGCTGGAGGAGAAGACACTGGGC GGTGAAGATGGTGACGTGTTTCATAATAGGACTACTCTTCCCCGTCTTCTCCGTGTGCTACCTGATAGCT CCCAAAAGCCCACTTGGACTGTTCATCAGAAAGCCATTTATCAAGTTTATCTGCCACACAGCCTCCTATC TGACCTTTTTGTTTCTGCTGCTGCTAGCCTCTCAGCACATCGACAGGTCAGACTTGAACAGGCAAGGTCC ACCACCAACCATCGTGGAGTGGATGATATTACCGTGGGTCCTGGGTAAATGTGTTACTCTAGAAAATATA ATAACGAACGTGTCTCTGCCGTGGAATTTTGTTGCTTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253683
Insert Size	1299 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253683.1, NP_001240612.1
RefSeq Size	2986 bp
RefSeq ORF	1299 bp
Locus ID	22066
Gene Summary	Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Acts as a cell-cell contact-dependent endothelial calcium entry channel. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. Trpc4 deficient mice lack a store-operated calcium entry in endothelial cells.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks several exons and its transcription extends past a splice site that is used in variant 1, resulting in a novel 3' coding region and 3' UTR compared to variant 1. It encodes isoform 3 which is shorter and has a distinct C-terminus compared to isoform 1.

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