Sept7 (NM_001205367) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Cdc10; E430034N22
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227693 representing NM_001205367 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGGTCAGTGCGAGATCCGCTGCTGCCGAGGAGAGGAGCGTCAACTGCGGCACCATGGCTCAACCGA AGAACCTTGAGGGCTATGTGGGCTTTGCCAACCTCCCAAATCAAGTGTACAGAAAATCCGTGAAAAGAGG ATTTGAATTCACTCTTATGGTAGTAGGTGAATCTGGACTGGGAAAGTCGACATTAATCAACTCATTATTC CTCACAGATTTGTATTCTCCAGAGTATCCAGGACCTTCTCATAGAATCAAAAAGACTGTACAGGTGGAGC AATCCAAAGTTTTAATCAAAGAAGGTGGTGTTCAGTTGCTGCTGACAATAGTTGATACTCCAGGATTTGG AGATGCAGTGGATAATAGTAATTGCTGGCAGCCTGTTATCGACTACATTGATAGTAAATTTGAAGATTAC TTAAATGCAGAATCTCGAGTGAACAGACGTCAGATGCCTGATAACAGGGTGCAGTGTTGTTTATACTTCA TTGCTCCTTCAGGACATGGACTTAAACCATTGGACATTGAGTTTATGAAACGTTTGCATGAAAAAGTGAA TATCATCCCATTAATTGCCAAAGCAGACACACTTACACCAGAGGAATGCCAACAGTTTAAAAAGCAGATA ATGAAAGAAATTCAAGAACATAAAATTAAAATATATGAATTTCCAGAAACAGATGATGAAGAAGAGAATA AACTGGTTAAGAAGATAAAGGACCGTTTACCTCTTGCTGTGGTAGGTAGTAATACTATCATTGAAGTTAA TGGCAAAAGAGTCAGAGGAAGGCAGTATCCTTGGGGTGTGGCTGAAGTTGAAAATGGTGAACATTGTGAT TTCACAATTCTAAGAAATATGTTGATAAGAACACATATGCAGGACTTGAAAGATGTTACCAATAACGTAC ACTATGAGAACTACAGAAGCAGAAAGCTAGCAGCAGTGACTTACAATGGAGTGGATAACAACAAGAACAA AGGGCAGCTTACCAAGAGCCCTCTGGCACAGATGGAAGAAGAAAGAAGGGAACATGTAGCCAAAATGAAG AAGATGGAGATGGAGATGGAACAGGTGTTTGAGATGAAGGTCAAAGAAAAAGTTCAAAAACTGAAGGACT CTGAAGCAGAGCTCCAGCGGCGCCATGAGCAAATGAAAAAGAATTTAGAAGCACAGCACAAAGAATTAGA GGAAAAACGTCGTCAGTTTGAAGAAGAAAAGGCAAACTGGGAAGCTCAACAGCGTATTTTAGAGCAACAG AACTCTTCAAGAACCTTGGAAAAGAACAAGAAGAAAGGCAAGATCTTTTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001205367
Insert Size	1311 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001205367.1, NP_001192296.1
RefSeq Size	2530 bp
RefSeq ORF	1311 bp
Locus ID	235072
UniProt ID	O55131
Gene Summary	Filament-forming cytoskeletal GTPase. Required for normal organization of the actin cytoskeleton. Required for normal progress through mitosis. Involved in cytokinesis. Required for normal association of CENPE with the kinetochore. Plays a role in ciliogenesis and collective cell movements. Forms a filamentous structure with SEPTIN12, SEPTIN6, SEPTIN2 and probably SEPTIN4 at the sperm annulus which is required for the structural integrity and motility of the sperm tail during postmeiotic differentiation (By similarity).[UniProtKB/Swiss-Prot Function]

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