Ednrb (NM_001276296) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ET-B; ET-BR; ETb; ETR-; ETR-b; Sox10; Sox10m1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227726 representing NM_001276296 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAATCGCCCGCAAGCCGGTGCGGACGCGCCTTGGTGGCGCTGCTGCTGGCCTGTGGCTTCTTGGGGG TATGGGGAGAGAAAAGAGGATTCCCACCTGCCCAAGCCACGCTGTCACTTCTCGGGACTAAAGAGGTAAT GACGCCACCCACTAAGACCTCCTGGACCAGAGGTTCCAACTCCAGTCTGATGCGTTCCTCCGCACCTGCG GAGGTGACCAAAGGAGGGAGGGGGGCTGGAGTCCCGCCAAGATCCTTCCCTCCTCCGTGCCAACGAAATA TTGAGATCAGCAAGACTTTTAAATACATCAACACGATTGTGTCGTGCCTCGTGTTCGTGCTAGGCATCAT CGGGAACTCCACGCTGCTAAGAATCATCTACAAGAACAAGTGCATGCGCAATGGTCCCAATATCTTGATC GCCAGTCTGGCTCTGGGAGACCTACTGCACATCATCATAGACATACCCATTAACACCTACAAGTTGCTCG CAGAGGACTGGCCATTTGGAGCTGAGATGTGTAAGCTGGTGCCCTTCATACAGAAGGCTTCTGTGGGAAT CACAGTGCTGAGTCTTTGTGCTCTAAGTATTGACAGATATCGAGCTGTTGCTTCTTGGAGTCGAATTAAA GGAATTGGGGTTCCAAAATGGACAGCAGTAGAAATTGTTTTAATTTGGGTGGTCTCTGTGGTTCTGGCTG TCCCCGAAGCCATAGGTTTTGATATGATTACGTCGGACTACAAAGGAAAGCCCCTAAGGGTCTGCATGCT TAATCCCTTTCAGAAAACAGCCTTCATGCAGTTTTACAAGACAGCCAAAGATTGGTGGCTGTTCAGTTTC TACTTCTGCTTGCCGCTAGCCATCACTGCAGTCTTTTATACCCTGATGACCTGCGAAATGCTCAGGAAGA AGAGCGGTATGCAGATTGCTTTGAATGATCACTTAAAGCAGAGACGAGAAGTGGCCAAGACAGTCTTCTG CCTGGTCCTCGTGTTTGCTCTCTGTTGGCTTCCCCTTCACCTCAGCCGGATCCTGAAGCTCACCCTGTAT GACCAGAGCAATCCACACAGGTGTGAGCTTCTGAGCTTTTTGTTGGTTTTGGACTACATTGGTATCAACA TGGCTTCTTTGAACTCCTGCATCAATCCAATCGCTCTGTATTTGGTGAGCAAAAGATTCAAAAACTGCTT TAAGTCATGTTTGTGCTGCTGGTGCCAAACGTTTGAGGAAAAGCAGTCCTTGGAGGAGAAGCAGTCCTGC CTGAAGTTCAAAGCCAACGATCACGGATATGACAACTTCCGGTCCAGCAATAAATACAGCTCGTCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001276296
Insert Size	1329 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001276296.1, NP_001263225.1
RefSeq Size	2788 bp
RefSeq ORF	1329 bp
Locus ID	13618
UniProt ID	P48302
Gene Summary	This gene encodes a member of the G-protein coupled receptor family. It encodes a receptor for endothelins, peptides that are involved in vasocontriction. The encoded protein activates a phosphatidylinositol-calcium second messenger system and is required for the development of enteric neurons and melanocytes. Gene disruption causes pigmentation anomalies, deafness, and abnormal dilation of the colon due to defects of neural crest-derived cells. Mutations in this gene are found in the piebald mouse, and mouse models of Hirschsprung's disease and Waardenburg syndrome type 4. Renal collecting duct-specific gene deletion causes sodium retention and hypertension. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2013] Transcript Variant: This variant (3) differs in the 5' and 3' UTRs compared to variant 2. Variants 1, 2, and 3 encode the same protein.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.