Deaf1 (NM_001282073) Mouse Untagged Clone
CAT#: MC227764
Deaf1 (untagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 3
CNY 4,370.00
货期*
3周
规格
Product images
经常一起买 (3)
Specifications
Product Data | |
Type | Mouse Untagged Clone |
Tag | Tag Free |
Synonyms | AU042387; C230009B13Rik; NUDR |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MC227764 representing NM_001282073
Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGACTTGCAGCTTCTTTGCAAAGGGAGAAAGAAGTGACCACAGTGACTGTGGCCAACGTGGGGTCCT CTGCAGATAACGTCTTCACAACATCAGTGGCAAACGCAGCATCGATATCAGGACATGTCCTGTCTGGTAG GACAGCCCTGCAGATCGGTGACAGCCTGAACACTGAAAAAGCCACACTAATAGTTGTACACACAGATGGG AGCATTGTGGAGACCACTGGGCTGAAGGGCCCAGCAGCACCTCTCACTCCAGGTCCCCAGTCTCCTCCTA CCCCACTGGCACCTGGCCAGGAGAAAGGTGGTACCAAGTACAACTGGGACCCTTCGGTGTATGACAGCGA GTTGCCTGTGCGCTGTCGGAACATCAGTGGCACGCTCTACAAGAGTAGGCTCGGCTCAGGTGGCCGGGGC CGGTGTATCAAGCAGGGAGAAAACTGGTACAGCCCAACTGAGTTTGAAGCTATGGCAGGAAGAGCCAGCA GCAAGGACTGGAAGAGAAGCATCCGCTATGCTGGCAGACCCCTGCAGTGCCTCATTCAGGATGGTATTTT GAACCCTCACGCTGCCTCCTGTACCTGTGCCGCCTGTTGTGATGACATGACTCTGAGTGGCCCTGTCAGG CTCTTCGTCCCTTATAAAAGGCGCAAGAAAGAGAATGAGCTGCCCACAACTCCAGTGAAGAAGGATTCCC CCAAGAACATCACCCTGCTTCCTGCCACGGCGGCCACCACCTTCACTGTGACACCCTCAGGACAGATCAC TACCTCTGGAGCACTGACCTTTGACAGAGCATCCACTGTAGAGGCCACTGCTGTCATCTCTGAGAGCCCA GCCCAAGGTGATGTCTTTGCAGGAGCCACAGTGCAAGAGGCAGGTGTGCAGCCTCCCTGCAGGGTTGGCC ACCCTGAACCCCACTACCCTGGCTATCAGGACAGCTGCCAGATTGCCCCGTTTCCAGAAGCTGCATTGCC AACATCACACCCCAAAATTGTCCTGACATCGCTGCCCGCATTGGCCGTGCCACCGTCCACCCCCACCAAA GCTGTCTCTCCCACCGTGGTCAGTGGGCTGGAGATGTCAGAACATCGGAGCTGGCTGTACCTGGAAGAGA TGGTCAACTCCCTACTCAACACAGCTCAGCAGCTGAAGACGCTGTTTGAACAAGCCAAGCAGGCGAGCTC TTGCAGGGAAGCTGCTGTGACCCAGGCGAGAATGCAGGTTGATACAGAGAGGAAAGAGGACTGGAAAGAC CATCAGCATGTGTGTGGCCAGTCAGCGTCTGTCACTGTCCAGGCTGATGACGTCCATGTTGAAGAAAGTG TGATAGAAAAAGTTGCTGTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001282073 |
Insert Size | 1353 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001282073.1, NP_001269002.1 |
RefSeq Size | 2032 bp |
RefSeq ORF | 1353 bp |
Locus ID | 54006 |
Gene Summary | Transcription factor that binds to sequence with multiple copies of 5'-TTC[CG]G-3' present in its own promoter and that of the HNRPA2B1 gene. Down-regulates transcription of these genes. Binds to the retinoic acid response element (RARE) 5'-AGGGTTCACCGAAAGTTCA-3'. Activates the proenkephalin gene independently of promoter binding, probably through protein-protein interaction (By similarity). Regulates epithelial cell proliferation and side-branching in the mammary gland. Required for neural tube closure and skeletal patterning. Controls the expression of peripheral tissue antigens in pancreatic lymph nodes. Isoform 1 displays greater transcriptional activity than isoform 2. Isoform 2 may inhibit transcriptional activity of isoform 1 by interacting with it and retaining it in the cytoplasm. Transcriptional activator of EIF4G3 (By similarity). May also involved in behavior (PubMed:24726472).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) represents use of an alternate promoter, differs in the 5' UTR, and has multiple differences in the coding region, compared to variant 1. The resulting protein (isoform 3) has a distinct N-terminus and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MR229975 | Deaf1 (myc-DDK-tagged) - Mouse deformed epidermal autoregulatory factor 1 (Drosophila) (Deaf1), transcript variant 3 |
CNY 4,470.00 |
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