Gfra1 (NM_001285457) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU042498; GDNFR; GFRalpha-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227828 representing NM_001285457 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCTAGCCACTCTGTACTTCGTGCTGCCACTCCTGGATTTGCTGATGTCGGCCGAGGTGAGTGGTG GGGACCGCCTGGACTGTGTGAAAGCCAGTGATCAGTGCCTGAAGGAACAGAGCTGCAGCACCAAGTACCG CACACTGAGGCAGTGCGTGGCGGGCAAGGAAACCAACTTCAGCCTGACATCCGGCCTCGAGGCCAAGGAT GAGTGCCGCAGCGCTATGGAGGCCTTGAAGCAGAAGTCTCTCTACAACTGCCGCTGCAAGCGGGGCATGA AGAAAGAGAAGAATTGTCTGCGTATCTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGACCTACTGGA AGATTCCCCATATGAGCCGGTTAACAGCAGGCTGTCAGATATATTCCGGGCAGTCCCGTTCATATCAGTG GAACACATTTCCAAAGGGAACAACTGCCTCGATGCAGCCAAGGCCTGCAACCTGGATGACACCTGCAAGA AGTACAGGTCCGCCTACATCACCCCCTGTACCACCAGCATGTCCAATGAAGTCTGCAACCGCCGCAAGTG CCACAAAGCCCTCAGGCAGTTCTTCGACAAAGTTCCAGCCAAGCACAGCTACGGGATGCTCTTCTGCTCC TGCCGGGACGTCGCCTGCACCGAGAGGCGGCGACAGACTATCGTCCCTGTGTGCTCCTATGAAGAACGAG AGAGGCCCAACTGCCTGAATCTGCAAGACTCCTGCAAGACAAATTACATCTGCAGATCTCGCCTTGCAGA TTTTTTTACCAACTGCCAGCCAGAGTCAAGGTCTGTCAGCAACTGTCTTAAGGAGAACTACGCAGACTGC CTCCTGGCCTACTCGGGACTGATTGGCACAGTCATGACTCCTAACTACATAGACTCCAGCAGCCTCAGTG TGGCGCCGTGGTGCGATTGCAGCAACAGTGGCAATGACCTGGAAGATTGCCTGAAGTTTCTGAATTTTTT TAAGGACAATACGTGTCTCAAAAATGCAATTCAAGCCTTTGGCAATGGCTCGGATGTGACCATGTGGCAG CCAGCCCCCCCAGTCCAGACCACCACTGCCACGACTACCACTGCCTTCCGGATCAAGAACAAGCCTCTAG GGCCAGCAGGCTCTGAGAATGAGATTCCCACACACGTTTTACCACCGTGTGCTAATTTGCAGGCACAGAA GCTGAAATCCAATGTATCGGGCAGTACACATCTCTGTCTTTCTGATAATGATTACGGAAAGGATGGTCTC GCTGGTGCCTCCAGCCACATAACCACAAAATCAATGGCTGCTCCTCCCAGCTGCGGTCTGAGCTCACTGC CGGTGATGGTGTTCACCGCTCTGGCTGCCCTGTTGTCTGTATCATTGGCAGAAACATCGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285457
Insert Size	1392 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285457.1, NP_001272386.1
RefSeq Size	4425 bp
RefSeq ORF	1392 bp
Locus ID	14585
UniProt ID	P97785
Gene Summary	This gene encodes a transmembrane protein that functions as the receptor for glial cell line derived neurotrophic factor (GDNF). The encoded protein undergoes proteolytic processing to generate a glycosylphosphatidylinositol-anchored cell surface coreceptor that forms a complex with the Ret tyrosine kinase in GDNF signaling pathway. Mice lacking the encoded protein exhibit deficits in the kidneys, the enteric nervous system, and spinal motor and sensory neurons similar mice deficient in GDNF or Ret. [provided by RefSeq, Jul 2016] Transcript Variant: This variant (3) has a shorter 5' UTR and lacks an exon in the central coding region but maintains the reading frame, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This isoform (2) may undergo proteolytic processing similar to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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