Agfg2 (NM_001303266) Mouse Untagged Clone

CAT#: MC227979

Agfg2 (untagged) - Mouse ArfGAP with FG repeats 2 (Agfg2), transcript variant 3



  "NM_001303266" in other vectors (1)

CNY 4,660.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms A630095P14Rik; Hr; Hrbl; RAB-R; RABR
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC227979 representing NM_001303266
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGATGGCGGCTAAGAAGGGCCCGGGCCCGGGTGGCGGGGTCGGAGGGAGCAAAGCGGAGGCTGAAG
CCGCTTCGGAGGTGTGGTGCCGCCGAGTGCGGGAGCTGGGCGGCTGCAGCCAGGCCGGGAACCGCCACTG
TTTCGAGTGCGCCCAGCGCGGGGTCACGTATGTGGACATCACCGTGGGCAGCTTCGTCTGCACCACCTGC
TCCGGCCTCCTGAGAGGCCTGAACCCCCCTCATCGAGTCAAGTCAATCTCCATGACAACTTTCACTGAGC
CTGAAGTCCTGTTCCTCCAATCTCGTGGAAATGAGGTCTGTCGGAAAATCTGGCTCGGTCTTTTTGATGC
TCGGACATCGTTGATACCAGATTCCAGGGATCCTCAGAAGGTGAAGGAGTTTCTCCAAGAAAAATATGAG
AAAAAAAGATGGTACGTCCCCCCAGAGCAAGTCAAGGGGCCCTCTTACAGCAAAGGCAGTGTCTCTGCTA
CCCCTGTCCAGGGCTCTGTCCCAGAAGGGAAACCCATTCGGACACTTCTGGGAGACCCTGTGCCATCTCT
CTCTGATCCTGCTTCCACTTCAAGCCAGCCTGGGAGCCAGTCGCAGGCACGCAGCAGCTCGCAGGCCAGG
AGCTCCCAGCCTCCTTCCCATTCATCCACCAAGAAAGCCAGCACTGACCTGCTGGCGGATATCGGGGGAG
ACCCCTTTGCTGCTCCCCAGGTGGTGCCAGCCTTTGCCTCATTCCCAGGCTTTGGAGTAGGCCAGACTCC
TGCCCATGGAGGCTTTGCCAACTTCGATGCCTTCAGCAGCAGCCCTAGCTCTTCCACCTTCGGAAGCCTC
CCTCCATCCGTCCAAGCGCCATTCCAGGCCCAGCCGACCCCTGCAGCCAATCGGATGCTAACTGGAAGTT
ACAGCTTTGGAAGTGGCCAGATGTCTGCGTTTGGTGTGGCACCCCTTGCAGCTGCCAGTCAACCCAACAA
CCTTGCAGATGTGGGCGGCCTCCTGGGTCCCAGGATGGCTGCTGGAGGTCTCCCTGGCAGTGTTTTTGGG
ATGCCGAGCCAGGTTCCTGCCCTGCAGTCGGCCGTGCCAGGTGTTAGCGGCAGTGGAGGGCTCCCCTTTG
GAGCCTACACCAACCCCTTCGCCACCCCTGCCCAAGCCCAGCTGCCTTCTACCAACCCATTCCAACCCAA
TGGTCTAGCCTCAGGGCCTGGCTTTGGGATGAGCAGTGTTCGGCCTGGCCTTCTCCAGCCAGTGCCACCC
TCCGGGGCCTTTGCCAGTCCCTTCTCTGCACCCGTGTTCCCCACACAGGCTGGACTGGCCGACCAGCAGA
ATGGATCTTCCTTTGGCGACTTGGGGACCTCTAAGCTGGGGCAGAGGCCACTGAGCCAGCCGGCTGGGAT
CTCTACCAATCCTTTCATGACTGGATCCTCAGCGTTTGCCTCCAAACCTCCAACCACAAACCCATTCTTG
TAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001303266
Insert Size 1473 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001303266.1, NP_001290195.1
RefSeq Size 2953 bp
RefSeq ORF 1473 bp
Locus ID 231801
Gene Summary This gene encodes a paralog of the HIV-1 Rev binding proteins that serve as cellular co-factors for HIV-1 Rev protein in shuttling viral pre-mRNAs from the nucleus to the cytoplasm. The encoded protein contains an ADP-ribosylation factor GTPase activating protein (Arf-GAP) zinc finger domain, several phenylalanine-glycine (FG) motifs and asparagine-proline-phenylalanine (NPF) motifs. Alternate splicing of this gene results in multiple transcript variants. [provided by RefSeq, Dec 2014]
Transcript Variant: This variant (3) contains an additional in-frame exon in the 3' coding region, compared to variant 2. The resulting protein (isoform 3) is longer than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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