Mdm4 (NM_001302801) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC227980 representing NM_001302801 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCACATTCCACCTCGGCCCAGTGTTCAGCATCTGACAGTGCTTGCAGAATTTCTTCGGAACAAA TTAGTCAGCAGGTGCGGCCAAAACTGCAGCTTTTGAAGATTTTGCATGCAGCAGGTGCGCAGGGGGAAGT ATTCACCATGAAAGAGGTAATGCACTATCTAGGCCAGTATATAATGGTGAAGCAGCTCTATGATCAACAG GAGCAACATATGGTATACTGTGGTGGAGATCTTTTGGGAGATCTACTTGGATGTCAGAGCTTTTCTGTGA AAGATCCAAGCCCTCTCTATGACATGCTAAGAAAGAATCTTGTTACATCAGCTTCTATTAACACAGATGC TGCTCAGACTCTCGCTCTCGCACAGGATCACACTATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGT GCAACAGAATACTCCAATCCCAGAAAAAGAACTGAAGAAGAGGATACTCACACACTGCCTACCTCACGAC ATAAATGCAGAGACTCCAGAGCAGATGAAGACTTGATAGAACATTTATCTCAAGATGAGACATCTAGGCT TGACCTTGATTTTGAGGAGTGGGACGTTGCTGGCCTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAAC TGTATTCCTAAAAGTAATGGCTCAACTGATTTACAGACAAATCAGGATATAGGTACTGCCATTGTTTCAG ACACTACGGATGATTTGTGGTTTTTAAATGAGACCGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGA AGCTGCTAATTCTGAGCAAACAAGTGAAGTAGGGAAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAG GATGATGATCTTGAGGACTCCAGGTCCTTGAGCGATGATACTGACGTGGAACTTACCTCTGAGGATGAGT GGCAGTGTACGGAATGCAAGAAGTTTAATTCTCCAAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAG AAAGGATTGGTATTCGGATTGTTCTAAATTAACTCATTCCCTATCTACATCTAATATTACTGCCATACCT GAAAAGAAGGACAATGAAGGAATTGATGTTCCCGATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGC CTAAAGATGGATATTTAAAGGAGGAAAAGCCCAGGTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTT GGCTCATAGTTCTGAAAGCCAGGAGATCATCTCAAGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAG AAAGCTGAAACAGAAAGTATGGAAGATTTCCAGAATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGC CTCGGGATGGGAACATTATTCATGGGAAGACGAGCCATCTGACGACATGTTTCCACTGTGCCAGGAGACT GAAGAAGTCTGGGGCTTCGTGTCCTGCTTGTAAGAAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCA TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302801
Insert Size	1473 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302801.1, NP_001289730.1
RefSeq Size	7223 bp
RefSeq ORF	1473 bp
Locus ID	17248
UniProt ID	O35618
Gene Summary	This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] Transcript Variant: This variant (1) encodes the longest isoform (1). Both variants 1 and 2 encode the same isoform (1).

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.