Mdm4 (NM_001302801) Mouse Tagged ORF Clone
CAT#: MR230191
- TrueORF®
Mdm4 (myc-DDK-tagged) - Mouse transformed mouse 3T3 cell double minute 4 (Mdm4), transcript variant 1
"NM_001302801" in other vectors (1)
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CNY 4,850.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; Mdmx |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR230191 representing NM_001302801
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACATCACATTCCACCTCGGCCCAGTGTTCAGCATCTGACAGTGCTTGCAGAATTTCTTCGGAACAAA TTAGTCAGCAGGTGCGGCCAAAACTGCAGCTTTTGAAGATTTTGCATGCAGCAGGTGCGCAGGGGGAAGT ATTCACCATGAAAGAGGTAATGCACTATCTAGGCCAGTATATAATGGTGAAGCAGCTCTATGATCAACAG GAGCAACATATGGTATACTGTGGTGGAGATCTTTTGGGAGATCTACTTGGATGTCAGAGCTTTTCTGTGA AAGATCCAAGCCCTCTCTATGACATGCTAAGAAAGAATCTTGTTACATCAGCTTCTATTAACACAGATGC TGCTCAGACTCTCGCTCTCGCACAGGATCACACTATGGATTTTCCAAGTCAAGACCGACTGAAGCACGGT GCAACAGAATACTCCAATCCCAGAAAAAGAACTGAAGAAGAGGATACTCACACACTGCCTACCTCACGAC ATAAATGCAGAGACTCCAGAGCAGATGAAGACTTGATAGAACATTTATCTCAAGATGAGACATCTAGGCT TGACCTTGATTTTGAGGAGTGGGACGTTGCTGGCCTGCCTTGGTGGTTTCTAGGGAATTTGAGAAACAAC TGTATTCCTAAAAGTAATGGCTCAACTGATTTACAGACAAATCAGGATATAGGTACTGCCATTGTTTCAG ACACTACGGATGATTTGTGGTTTTTAAATGAGACCGTGTCAGAGCAATTAGGTGTTGGAATAAAAGTTGA AGCTGCTAATTCTGAGCAAACAAGTGAAGTAGGGAAAACAAGTAACAAGAAGACGGTGGAGGTGGGAAAG GATGATGATCTTGAGGACTCCAGGTCCTTGAGCGATGATACTGACGTGGAACTTACCTCTGAGGATGAGT GGCAGTGTACGGAATGCAAGAAGTTTAATTCTCCAAGCAAGAGGTACTGTTTTCGTTGCTGGGCCTTGAG AAAGGATTGGTATTCGGATTGTTCTAAATTAACTCATTCCCTATCTACATCTAATATTACTGCCATACCT GAAAAGAAGGACAATGAAGGAATTGATGTTCCCGATTGTAGGAGAACCATTTCAGCTCCTGTTGTTAGGC CTAAAGATGGATATTTAAAGGAGGAAAAGCCCAGGTTTGACCCTTGCAACTCAGTGGGATTTTTGGATTT GGCTCATAGTTCTGAAAGCCAGGAGATCATCTCAAGCGCGAGAGAACAAACAGATATTTTTTCTGAGCAG AAAGCTGAAACAGAAAGTATGGAAGATTTCCAGAATGTCTTGAAGCCGTGTAGCTTATGTGAAAAAAGGC CTCGGGATGGGAACATTATTCATGGGAAGACGAGCCATCTGACGACATGTTTCCACTGTGCCAGGAGACT GAAGAAGTCTGGGGCTTCGTGTCCTGCTTGTAAGAAAGAGATTCAGTTGGTTATTAAAGTTTTTATAGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR230191 representing NM_001302801
Red=Cloning site Green=Tags(s) MTSHSTSAQCSASDSACRISSEQISQQVRPKLQLLKILHAAGAQGEVFTMKEVMHYLGQYIMVKQLYDQQ EQHMVYCGGDLLGDLLGCQSFSVKDPSPLYDMLRKNLVTSASINTDAAQTLALAQDHTMDFPSQDRLKHG ATEYSNPRKRTEEEDTHTLPTSRHKCRDSRADEDLIEHLSQDETSRLDLDFEEWDVAGLPWWFLGNLRNN CIPKSNGSTDLQTNQDIGTAIVSDTTDDLWFLNETVSEQLGVGIKVEAANSEQTSEVGKTSNKKTVEVGK DDDLEDSRSLSDDTDVELTSEDEWQCTECKKFNSPSKRYCFRCWALRKDWYSDCSKLTHSLSTSNITAIP EKKDNEGIDVPDCRRTISAPVVRPKDGYLKEEKPRFDPCNSVGFLDLAHSSESQEIISSAREQTDIFSEQ KAETESMEDFQNVLKPCSLCEKRPRDGNIIHGKTSHLTTCFHCARRLKKSGASCPACKKEIQLVIKVFIA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001302801 |
ORF Size | 1470 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001302801.1, NP_001289730.1 |
RefSeq Size | 7223 bp |
RefSeq ORF | 1473 bp |
Locus ID | 17248 |
UniProt ID | O35618 |
MW | 55.5 kDa |
Gene Summary | This gene encodes a protein that has been shown to negatively regulate the activity of the tumor suppressor protein p53. Homozygous knockout mice exhibit embryonic lethality as a result of p53-dependent apoptosis and cell cycle arrest. Amplification of this gene or overexpression of the encoded protein has been linked to a range of human cancers. A pseudogene has been identified on the X chromosome. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC227980 | Mdm4 (untagged) - Mouse transformed mouse 3T3 cell double minute 4 (Mdm4), transcript variant 1 |
CNY 4,660.00 |