Esr1 (NM_001302533) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	E; ER; ER-; ER-alpha; ERa; ERalpha; ER[; Es; ESR; Estr; Estra; Nr; Nr3a1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228048 representing NM_001302533 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCATGACCCTTCACACCAAAGCCTCGGGAATGGCCTTGCTGCACCAGATCCAAGGGAACGAGCTGG AGCCCCTCAACCGCCCGCAGCTCAAGATGCCCATGGAGAGGGCCCTGGGCGAGGTATACGTGGACAACAG CAAGCCCACTGTGTTCAACTACCCCGAGGGCGCCGCCTACGAGTTCAACGCCGCCGCCGCCGCCGCCGCC GCCGCCTCGGCGCCGGTCTACGGCCAGTCGGGCATCGCCTACGGCCCCGGGTCGGAGGCGGCCGCCTTCA GTGCCAACAGCCTGGGGGCTTTCCCCCAGCTCAACAGCGTGTCGCCTAGCCCGCTGATGCTGCTGCACCC GCCGCCGCAGCTGTCTCCTTTCCTGCACCCGCACGGCCAGCAGGTGCCCTACTACCTGGAGAACGAGCCC AGCGCCTACGCCGTGCGCGACACCGGCCCTCCCGCCTTCTACAGGTCTAATTCTGACAATCGACGCCAGA ATGGCCGAGAGAGACTGTCCAGCAGTAACGAGAAAGGAAACATGATCATGGAGTCTGCCAAGGAGACTCG CTACTGTGCCGTGTGCAATGACTATGCCTCTGGCTACCATTATGGGGTCTGGTCCTGCGAAGGCTGCAAG GCTTTCTTTAAGAGAAGCATTCAAGGACACAATGACTACATGTGTCCAGCTACAAACCAATGCACCATTG ACAAGAACCGGAGGAAGAGTTGCCAGGCCTGTCGGCTGCGCAAGTGTTACGAAGTGGGCATGATGAAAGG CGGCATACGGAAAGACCGCCGAGGAGGGAGAATGTTGAAGCACAAGCGTCAGAGAGATGACTTGGAAGGC CGAAATGAAATGGGTGCTTCAGGAGACATGAGGGCTGCCAACCTTTGGCCAAGCCCTCTTGTGATTAAGC ACACTAAGAAGAATAGCCCTGCCTTGTCCTTGACAGCTGACCAGATGGTCAGTGCCTTGTTGGATGCTGA ACCGCCCATGATCTATTCTGAATATGATCCTTCTAGACCCTTCAGTGAAGCCTCAATGATGGGCTTATTG ACCAACCTAGCAGATAGGGAGCTGGTTCATATGATCAACTGGGCAAAGAGAGTGCCAGGCTTTGGGGACT TGAATCTCCATGATCAGGTCCACCTTCTCGAGTGTGCCTGGCTGGAGATTCTGATGATTGGTCTCGTCTG GCGCTCCATGGAACACCCGGGGAAGCTCCTGTTTGCTCCTAACTTGCTCCTGGACAGAGAACATTCTGTC AAGACTTTGAAAAGAAGCTCTGACAAGGACCTACAAGGACTTCAGACAACTGGGATTCCTTTATGTCAGA AAGAAGATGGACTACTTTTAAAAAATGCAACCAGGGTACAAGAAAAACCAACCTGTAGTCTGCACTCTCT AACCAAGGCCAAGAGAGCCTGCCATGTGATACTAGTCCCATCCCCAGAGTCCAGAAATGTAGGTGTCAAA GATTCCACTCTGCTCGTAGTGAACTCCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302533
Insert Size	1500 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302533.1, NP_001289462.1
RefSeq Size	2730 bp
RefSeq ORF	1500 bp
Locus ID	13982
Gene Summary	This gene encodes an estrogen receptor, a member of the nuclear hormone family of intracellular receptors. The encoded protein, activated by the sex hormone estrogen, is a transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Similar genes in human have been implicated in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (4) differs in the 5' UTR, contains an alternate exon in the 3' coding region and uses an upstream alternate 3' terminal exon, compared to variant 1. The encoded isoform (2) has a shorter and distinct C-terminus, compared to isoform 1.

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