Tfcp2 (NM_001289603) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	CP-2; CP2; D230015P20Rik; LBP-1c; LBP-1d; LBP1; LSF; Tcfcp2; UBP-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228050 representing NM_001289603 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCTGGGCTCTGAAGCTGCCGCTAGCCGACGAAGTGATCGAGTCGGGGCTTGTGCAGGACTTTGATG CTAGCCTGTCCGGCATTGGCCAGGAACTGGGTGCTGGTGCTTATAGTATGAGTGATGTCCTCGCATTGCC CATTTTTAAGCAAGAAGAGTCAAGTTTGCCTCCCGATAATGAGAACGAGATTCTGCCTTTTCAGTATGTG CTCTGTGCTGCCACGTCTCCAGCGGTGAAGCTCCACGACGAGACCCTGACGTACCTCAATCAAGGACAGT CTTATGAAATCCGAATGCTAGACAATAGAAAACTGGGAGAGCTTCCAGAACTCAACGGCAAGCTGGTGAA GAGCATATTCCGCGTAGTGTTCCACGACAGGCGACTGCAGTACACTGAGCACCAGCAGCTGGAGGGCTGG AGGTGGAACCGACCGGGAGACAGGATTCTCGACATAGATATCCCAATGTCTGTGGGTGTAATTGATCCCA GGGCCAATCCTACCCAGCTAAACACAGTGGAGTTCCTGTGGGACCCTTCAAAGAGGACATCTGTGTTTAT TCAGGTGCACTGCATCAGCACTGAGTTCACCATGAGGAAGCATGGCGGGGAGAAGGGGGTGCCGTTCCGA GTGCAGATCGATACCTTCAAGGAGAACGGGAACGGAGAGTACACAGAGCACTTGCACTCGGCCAGCTGCC AGATCAAAGTCTTCAAGCCTAAGGGTGCAGACAGAAAACAAAAAATAGATAGAGAGAAAATGGAGAAACG GACGCCCCATGAAAAAGAGAAATATCAACCTTCTTATGAGACAACCATACTCACCGAGTGTTCTCCGTGG CCTGAGATCACCTACGTCAACAATTCCCCATCCCCTGGCTTCAACAGCTCCCATAGCAGTTTCTCTCTTG GGGAAGGAAACGGCTCGCCCAACCACCAGCCAGAACCACCCCCTCCAGTCACGGATAACCTCTTGCCGAC AACCACGCCTCAGGAAGCCCAGCAATGGCTGCATCGAAACCGGTTCTCCACATTCACGAGGCTTTTCACC AACTTCTCAGATTTACTGAAACTAACTAGAGACGACGTGATCCAAATCTGCGGCCCTGCAGATGGAATCA GACTCTTTAATGCATTAAAAGGCCGGATGGTGCGGCCAAGGCTAACCATTTATGTCTGCCAGGAGTCGTT GCAGTTGAGGGAGCAGCAGCCGCAGCCGCAGCCGCAGCCGCAGAAGCAGGAGGATGGGGATTCAAACGGT ACTTTCTTCGTGTACCATGCTATCTATCTCGAGGAGCTGACAGCTGTCGAACTCACAGAAAAAATTGCTC AGCTTTTCAGCATTTCTCCTCACCAGATCAGCCAGATTTACAAGCAAGGGCCGACAGGGATCCATGTGGT CATCAGCGATGAGATGGTACAGAACTTCCAGGAAGAAGCCTGCTTTATTCTGGACACGATGGAAGCAGAA ACCAGTGACAGCTATCATGTCATTCTCAAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289603
Insert Size	1503 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289603.1, NP_001276532.1
RefSeq Size	3295 bp
RefSeq ORF	1503 bp
Locus ID	21422
UniProt ID	Q9ERA0
Gene Summary	Binds a variety of cellular promoters including fibrinogen, alpha-globin promoters. Activation of the alpha-globin promoter in erythroid cells is via synergistic interaction with UBP1 (By similarity). Functions as part of the SSP (stage selector protein) complex. Facilitates the interaction of the gamma-globin genes with enhancer elements contained in the locus control region in fetal erythroid cells. Interacts by binding to the stage selector element (SSE) in the proximal gamma-globin promoter (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice in the 3' coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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