Pde1b (NM_001285890) Mouse Untagged Clone

CAT#: MC228128

Pde1b (untagged) - Mouse phosphodiesterase 1B, Ca2+-calmodulin dependent (Pde1b), transcript variant 2



  "NM_001285890" in other vectors (1)

CNY 5,040.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
PDE1B Antibody - middle region
    • 50 ug

CNY 4,628.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms Pde1b1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228128 representing NM_001285890
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCAAACCCTGTTCCTGTCCAGAGGAGCCACCTCCAGGGCCCCATCCTCAGGCTGCGCTACATGGTGA
AGCAGTTGGAGAATGGGGAGGTTAACATCGAGGAGCTGAAGAAAAACCTGGAATACACAGCTTCCCTGCT
GGAAGCTGTCTACATTGATGAGACAAGGCAAATCCTAGACACTGAAGATGAGCTTCGGGAACTTCGGTCA
GATGCTGTGCCTTCAGAGGTGCGGGACTGGTTGGCCTCCACCTTCACCCAGCAGACCCGAGCCAAAGGTC
GCAGGGCAGAAGAGAAACCCAAGTTCCGAAGCATCGTGCATGCTGTGCAGGCTGGGATCTTCGTGGAGCG
GATGTTCCGGAGAACGTATACCTCTGTGGGCCCCACATACTCTACTGCAGTCCACAACTGTCTCAAGAAC
CTGGACCTCTGGTGCTTTGATGTCTTTTCCTTGAACCGGGCAGCCGATGACCACGCTCTGAGGACCATTG
TTTTTGAGTTGCTGACTCGGCATAGCCTCATCAGCCGCTTTAAGATTCCCACAGTGTTTCTGATGAGTTT
TCTGGAGGCCTTGGAGACAGGCTATGGGAAATATAAGAATCCTTACCACAACCAGATCCACGCAGCCGAC
GTGACCCAGACTGTCCATTGCTTCCTTCTCCGCACAGGCATGGTGCACTGCCTGTCAGAGATTGAGGTCT
TGGCCATCATCTTTGCTGCAGCCATCCATGACTATGAGCACACAGGCACAACCAACAGCTTCCACATTCA
GACCAAGTCAGAATGTGCCATCCTGTACAATGATCGATCGGTGCTGGAGAATCACCACATCAGCTCTGTC
TTTCGAATGATGCAGGATGATGAGATGAACATTTTTATCAATCTCACCAAGGATGAATTTGCAGAGCTGC
GGGCCCTGGTTATCGAGATGGTGTTGGCCACAGACATGTCCTGCCATTTCCAGCAAGTGAAGACTATGAA
GACAGCACTGCAGCAGTTGGAAAGGATTGACAAGTCCAAGGCCCTATCTCTTCTGCTTCATGCTGCTGAC
ATCAGCCACCCAACTAAGCAGTGGTCAGTCCACAGCCGCTGGACCAAGGCCCTAATGGAAGAGTTCTTCC
GCCAGGGTGACAAGGAGGCAGAGCTGGGCCTGCCCTTCTCTCCACTCTGTGATCGCACTTCCACATTGGT
GGCCCAGTCCCAGATAGGTTTCATTGACTTCATTGTGGAGCCAACCTTCTCTGTGCTGACTGATGTGGCA
GAAAAGAGTGTCCAGCCCTTGGCAGATGATGATTCCAAGCCTAAAAGTCAGCCCAGCTTCCAGTGGCGCC
AGCCTTCTTTAGATGTGGACGTAGGAGACCCCAACCCTGATGTGGTCAGTTTCCGTGCCACCTGGACCAA
GTACATTCAAGAGAACAAACAGAAGTGGAAGGAACGGGCAGCAAGTGGCATCACCAACCAGATGTCCATT
GATGAACTGTCCCCCTGTGAGGAAGAAGCCCCATCCTCCCCTGCAGAAGATGAGCACAACCAGAATGGGA
ATCTGGATTAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001285890
Insert Size 1551 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001285890.1, NP_001272819.1
RefSeq Size 3245 bp
RefSeq ORF 1551 bp
Locus ID 18574
Gene Summary Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. Has a preference for cGMP as a substrate (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and contains an alternate 5' terminal exon compared to variant 1. The encoded protein (isoform 2) has a distinct N-terminus and is shorter than isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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