Pkm (NM_001253883) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AA414905; AL024370; AL024424; Pk-2; Pk-3; Pk3; Pkm2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228197 representing NM_001253883 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAAGCCACACAGTGAAGCAGGGACTGCCTTCATTCAGACCCAGCAGCTCCATGCAGCCATGGCTG ACACCTTCCTGGAACACATGTGCCGCCTGGACATTGACTCTGCCCCCATCACGGCCCGCAACACTGGCAT CATTTGTACCATTGGGCCTGCTTCCCGATCTGTGGAGATGCTGAAGGAGATGATTAAGTCTGGAATGAAT GTGGCTCGGCTGAATTTCTCTCATGGAACCCATGAGTACCATGCAGAGACCATCAAGAATGTCCGTGAAG CCACAGAAAGCTTTGCATCTGATCCCATTCTCTACCGTCCTGTTGCGGTGGCTCTGGATACAAAGGGACC TGAGATCCGGACTGGACTCATCAAGGGCAGCGGCACCGCTGAGGTGGAGCTGAAGAAGGGAGCCACTCTG AAGATCACCCTGGACAACGCTTACATGGAGAAGTGTGACGAGAACATCCTGTGGCTGGACTACAAGAACA TCTGCAAGGTGGTGGAGGTGGGCAGCAAGATCTACGTGGACGATGGGCTCATCTCACTGCAGGTGAAGGA GAAAGGCGCTGACTTCCTGGTGACGGAGGTGGAGAATGGTGGCTCCTTGGGCAGCAAGAAGGGCGTGAAC CTGCCGGGCGCTGCTGTGGATCTCCCCGCTGTGTCGGAAAAGGACATCCAGGACCTGAAGTTTGGGGTGG AGCAGGATGTGGACATGGTGTTTGCATCTTTCATCCGCAAGGCAGCCGACGTGCATGAAGTCAGGAAGGT GCTGGGAGAGAAGGGCAAGAACATCAAGATCATCAGCAAAATCGAGAACCATGAAGGCGTCCGCAGGTTT GATGAGATCTTGGAGGCCAGTGATGGGATCATGGTGGCTCGTGGTGACCTGGGCATTGAGATTCCTGCAG AGAAGGTCTTCCTGGCTCAGAAGATGATGATCGGGCGATGCAACCGAGCTGGGAAGCCTGTCATCTGTGC CACACAGATGCTGGAGAGCATGATCAAGAAGCCACGCCCCACCCGTGCTGAAGGCAGTGATGTGGCCAAT GCAGTCCTGGATGGAGCAGACTGCATCATGCTGTCTGGAGAAACAGCCAAGGGGGACTACCCTCTGGAGG CTGTTCGCATGCAGCACCTGATAGCTCGGGAGGCTGAGGCAGCCATGTTCCACCGTCTGCTGTTTGAAGA GCTTGTGCGAGCCTCCAGTCACTCCACAGACCTCATGGAGGCCATGGCCATGGGCAGCGTGGAGGCCTCT TATAAGTGTTTAGCAGCAGCTTTGATAGTTCTCACGGAGTCTGGCAGGAGTGCTCACCAAGTGGCCAGGT ACCGCCCTCGGGCTCCTATCATTGCCGTGACTCGAAATCCCCAGACTGCTCGCCAGGCCCATCTGTACCG TGGCATCTTCCCTGTGCTGTGTAAGGATGCCGTGCTGAATGCCTGGGCTGAGGATGTCGACCTTCGTGTA AACTTGGCCATGGATGTTGGCAAGGCCCGAGGCTTCTTCAAGAAGGGAGATGTGGTCATTGTGCTGACCG GGTGGCGCCCTGGCTCTGGATTCACCAACACCATGCGTGTAGTGCCTGTACCTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001253883
Insert Size	1596 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001253883.1, NP_001240812.1
RefSeq Size	2432 bp
RefSeq ORF	1596 bp
Locus ID	18746
UniProt ID	P52480
Gene Summary	Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP (By similarity). Stimulates POU5F1-mediated transcriptional activation (By similarity). Promotes in a STAT1-dependent manner, the expression of the immune checkpoint protein CD274 in ARNTL/BMAL1-deficient macrophages (PubMed:29996098).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) encodes isoform M1. Isoforms M1 and M2 are identical in length but have different sequences.

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