Ash2l (NM_001286207) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228209 representing NM_001286207 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACCCAGGCGGGCTCTGTGGATGAGGAGAATGGGCGGCAGTTGGGAGAAGTGGAGTTGCAGTGTG GGATATGTACAAAATGGTTCACCGCTGACACCTTTGGAATAGACACGTCGTCATGTCTGCCTTTTATGAC CAACTACAGCTTCCATTGCAATGTGTGCCATCACAGTGGGAATACCTACTTTCTCCGGAAGCAAGCAAAT TTAAAAGAAATGTGCCTTAGTGCCTTGGCGAATTTGACGTGGCAGTCTCGAACACAGGATGAACATCCAA AAACCATGTTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAATGTATGACAACCAG ACAGAGACCTGGAAAAATGACTTGGCCCAATAACATTGTCAAAACAATGAGTAAGGAAAGAGATGTGTTC TTGGTAAAGGAACACCCTGACCCAGGAAGCAAAGACCCAGAAGAAGATTACCCCAAGTTTGGACTTTTGG ATCAGGATCTTAGTAATATTGGTCCTGCTTATGACAACCAGAAACAAAGCAGTGCTGTGTCTGCTAGTGG GAACCTAAATGGGGGGATCGCAGCCGGAAGCAGTGGGAAAGGAAGAGGAGCCAAGCGTAAGCAGCAAGAT GGAGGGACAACAGGGACCACCAAGAAGGCCAGAAGTGATCCTTTATTTTCTGCTCAGCGTCTCCCTCCTC ATGGCTATCCTTTGGAACATCCATTTAACAAAGATGGCTATCGGTATATTCTTGCTGAGCCCGATCCTCA TGCCCCCGACCCGGAGAAGCTTGAACTTGACTGCTGGGCGGGAAAGCCTATTCCTGGAGACCTTTACAGA GCCTGCTTATATGAACGAGTCTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCTGATGACC GGCTGACCGTGGTTGGAGAGAAGGGCTACTCCATGGTCCGGGCCTCTCATGGGGTACGCAAAGGGGCCTG GTACTTTGAAATCACTGTGGATGAGATGCCCCCAGACACTGCTGCCAGGCTGGGCTGGTCCCAGCCCTTA GGTAACCTCCAAGCTCCCTTAGGCTATGATAAGTTTAGCTATTCTTGGCGGAGCAAGAAAGGCACCAAGT TCCACCAGTCCATTGGCAAGCACTATTCGTCTGGCTACGGACAAGGGGACGTCCTAGGGTTCTATATCAA CCTTCCCGAAGACACAGAGACAGCCAAGTCACTGCCGGACACCTACAAAGATAAGGCTTTGATAAAGTTC AAGAGTTATTTGTATTTTGAAGAAAAAGACTTTGTGGACAAAGCAGAGAAGAGCCTAAAACAGACCCCCC ATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAGGGTGTGGCTTACAGAGATATTTTTGAAGGAGT TTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCATCCTTCAAATAC CCTCCAAAGGATCTCACGTACCACCCTATGAGTGACATGGGCTGGGGCGCTGTGGTAGAACACACACTGG CTGATGTTTTGTATCACGTGGAGACAGAAGTGGACGGAAGACGTAGTCCACCCTGGGAACCCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001286207
Insert Size	1605 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286207.1, NP_001273136.1
RefSeq Size	3186 bp
RefSeq ORF	1605 bp
Locus ID	23808
Gene Summary	Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR, has multiple differences in the coding region, and initiates translation at a downstream start codon, compared to variant 4. The resulting isoform (b) has, among multiple differences, a shorter N-terminus compared to isoform d. This variant (3), as well as variant 2, encodes isoform b.

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