Ash2l (NM_001286207) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230420 representing NM_001286207 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATACCCAGGCGGGCTCTGTGGATGAGGAGAATGGGCGGCAGTTGGGAGAAGTGGAGTTGCAGTGTG GGATATGTACAAAATGGTTCACCGCTGACACCTTTGGAATAGACACGTCGTCATGTCTGCCTTTTATGAC CAACTACAGCTTCCATTGCAATGTGTGCCATCACAGTGGGAATACCTACTTTCTCCGGAAGCAAGCAAAT TTAAAAGAAATGTGCCTTAGTGCCTTGGCGAATTTGACGTGGCAGTCTCGAACACAGGATGAACATCCAA AAACCATGTTCTCCAAAGATAAGGATATTATACCATTTATTGATAAATACTGGGAATGTATGACAACCAG ACAGAGACCTGGAAAAATGACTTGGCCCAATAACATTGTCAAAACAATGAGTAAGGAAAGAGATGTGTTC TTGGTAAAGGAACACCCTGACCCAGGAAGCAAAGACCCAGAAGAAGATTACCCCAAGTTTGGACTTTTGG ATCAGGATCTTAGTAATATTGGTCCTGCTTATGACAACCAGAAACAAAGCAGTGCTGTGTCTGCTAGTGG GAACCTAAATGGGGGGATCGCAGCCGGAAGCAGTGGGAAAGGAAGAGGAGCCAAGCGTAAGCAGCAAGAT GGAGGGACAACAGGGACCACCAAGAAGGCCAGAAGTGATCCTTTATTTTCTGCTCAGCGTCTCCCTCCTC ATGGCTATCCTTTGGAACATCCATTTAACAAAGATGGCTATCGGTATATTCTTGCTGAGCCCGATCCTCA TGCCCCCGACCCGGAGAAGCTTGAACTTGACTGCTGGGCGGGAAAGCCTATTCCTGGAGACCTTTACAGA GCCTGCTTATATGAACGAGTCTTGTTAGCCCTACATGATCGAGCTCCCCAGTTAAAGATCTCTGATGACC GGCTGACCGTGGTTGGAGAGAAGGGCTACTCCATGGTCCGGGCCTCTCATGGGGTACGCAAAGGGGCCTG GTACTTTGAAATCACTGTGGATGAGATGCCCCCAGACACTGCTGCCAGGCTGGGCTGGTCCCAGCCCTTA GGTAACCTCCAAGCTCCCTTAGGCTATGATAAGTTTAGCTATTCTTGGCGGAGCAAGAAAGGCACCAAGT TCCACCAGTCCATTGGCAAGCACTATTCGTCTGGCTACGGACAAGGGGACGTCCTAGGGTTCTATATCAA CCTTCCCGAAGACACAGAGACAGCCAAGTCACTGCCGGACACCTACAAAGATAAGGCTTTGATAAAGTTC AAGAGTTATTTGTATTTTGAAGAAAAAGACTTTGTGGACAAAGCAGAGAAGAGCCTAAAACAGACCCCCC ATAGTGAGATAATATTTTATAAAAATGGTGTCAATCAGGGTGTGGCTTACAGAGATATTTTTGAAGGAGT TTACTTCCCAGCCATCTCACTGTACAAGAGCTGCACGGTTTCCATTAACTTTGGACCATCCTTCAAATAC CCTCCAAAGGATCTCACGTACCACCCTATGAGTGACATGGGCTGGGGCGCTGTGGTAGAACACACACTGG CTGATGTTTTGTATCACGTGGAGACAGAAGTGGACGGAAGACGTAGTCCACCCTGGGAACCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230420 representing NM_001286207 Red=Cloning site Green=Tags(s) MDTQAGSVDEENGRQLGEVELQCGICTKWFTADTFGIDTSSCLPFMTNYSFHCNVCHHSGNTYFLRKQAN LKEMCLSALANLTWQSRTQDEHPKTMFSKDKDIIPFIDKYWECMTTRQRPGKMTWPNNIVKTMSKERDVF LVKEHPDPGSKDPEEDYPKFGLLDQDLSNIGPAYDNQKQSSAVSASGNLNGGIAAGSSGKGRGAKRKQQD GGTTGTTKKARSDPLFSAQRLPPHGYPLEHPFNKDGYRYILAEPDPHAPDPEKLELDCWAGKPIPGDLYR ACLYERVLLALHDRAPQLKISDDRLTVVGEKGYSMVRASHGVRKGAWYFEITVDEMPPDTAARLGWSQPL GNLQAPLGYDKFSYSWRSKKGTKFHQSIGKHYSSGYGQGDVLGFYINLPEDTETAKSLPDTYKDKALIKF KSYLYFEEKDFVDKAEKSLKQTPHSEIIFYKNGVNQGVAYRDIFEGVYFPAISLYKSCTVSINFGPSFKY PPKDLTYHPMSDMGWGAVVEHTLADVLYHVETEVDGRRSPPWEP TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286207
ORF Size	1602 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286207.1, NP_001273136.1
RefSeq Size	3186 bp
RefSeq ORF	1605 bp
Locus ID	23808
MW	60.2 kDa
Gene Summary	Component of the Set1/Ash2 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3, but not if the neighboring 'Lys-9' residue is already methylated. As part of the MLL1/MLL complex it is involved in methylation and dimethylation at 'Lys-4' of histone H3. May function as a transcriptional regulator. May play a role in hematopoiesis (By similarity).[UniProtKB/Swiss-Prot Function]

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