Skil (NM_001271772) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	S; Skir; sno; sno-; SnoN
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228258 representing NM_001271772 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACCTCCAGTCTAAGTTCTCCTTAGTTCAGGGTTCAAATAAAAAGCTGAACGGCATGGAGGATG ATGGCAGTCCTCCTGTGAAAAAAATGATGACCGACATTCATGCCAATGGGAAAACCCTGACCAAGGTTCT TCCCGGCCCTCTTCTCATTCCTTCTGATAGCTCCACAGAGCTCACTCAGACTCTTTTGGAAGGGGAGTCT ATTTCTTGTTTTCAAGTTGGAGGAGAAAAGAGACTCTGTTTGCCCCAAGTCTTAAACTCTGTCCTCCGAG AATTTTCACTCCAGCAGATAAACACAGTATGTGATGAGCTGTACATCTATTGTTCAAGGTGTACTTCAGA CCAGCTTCATATCTTAAAGGTCTTAGGGATACTCCCATTTAATGCTCCATCCTGTGGGCTGATCACGTTG ACCGATGCACAAAGACTCTGTAATGCTTTACTGCGGCCACGAACTTTTCCTCAAAATGGTAGCATACTTC CTGCTAAAAGCTCTCTGGCCCAGTTGAAGGAAACTGGCAGTGCCTTTGAAGTGGAACATGAATGCTTGGG CAAATGTCAGGGTCTCTTTGCGCCTCAGTTTTATGTTCAGCCCGACGCTCCCTGTATCCAGTGCCTGGAG TGCTGTGGAATGTTTGCTCCCCAGACGTTCGTCATGCATTCGCACAGATCCCCTGACAAGAGAACTTGCC ATTGGGGCTTTGAGTCAGCCAAGTGGCACTGTTACCTTCATGTGAACCAAAAATACCTAGGGACACCCGA AGAAAAGAAACTGAAGATAATTTTAGAAGAAATGAAGGAGAAGTTTAGCATGAGAAATGGAAAGAGAATC CAATCAAAGACAGATACACCATCGGGAATGGAATTGCCATCATGGTATCCTGTTATAAAACAGGAAGGTG ACCATGTTCCCCAGACACATTCATTTTTACACCCCAGCTACTACCTGTACATGTGTGACAAAGTGGTTGC ACCGAATGTGTCGCTCACATCTGCTGCGTCCCAGTCTAAAGAGGCCACCAAGGCAGAGACAAATTCATCA ATCTCAAATAATTCTACAAGTAGAAAGAAATCTGAGTCTGCTGTGTGCAGCTTAGTGAGAGGCACAAGCA AGCGTGATTCAGAAGATTCCTCTCCACTTCTGGTCAGAGATGGTGAAGACGACAAGGGGAAGATCATGGA AGACGTGATGAGAACCTATGTAAGACAGCAGGAGAAGCTGAACTCCATTCTGCAGAGGAAGCAGCAGCTG CAGATGGAGGTGGAGATGCTGAGCAGTTCAAAGGCGATGAAGGAGCTCACTGAAGAGCAGCAGAATCTAC AGAAAGAGCTTGAGTCGTTACAGAGTGAGCATGCTCAGAGGATGGAGGAATTCTATATTGAACAGAGAGA CTTAGAGAAAAAGCTGGAGCAGGTGATGCAGCAGAAATGCACCTGTGACTCAACCTTGGAGAAGGACAGG GAGGCCGAGTATGCGGCTCAGCTGGCAGAACTGAGGCAAAGACTAGACCATGCTGAGGCCGACAGGCAGG AACTCCAAGATGAACTCAGACAGGAGCGGGAGGCGAGACAGAAGCTGGAGATGATGATAAAGGAGCTGAA GCTGCAGATTGGCAAGTCGTCCAAGCCCTCCAAGGACTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271772
Insert Size	1650 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271772.1, NP_001258701.1
RefSeq Size	6609 bp
RefSeq ORF	1650 bp
Locus ID	20482
Gene Summary	This gene encodes a member of a small family of proteins that play a key role in the response of cells to extracellular growth signals. The encoded protein regulates members of the transforming growth factor beta signaling pathway. It is highly expressed in certain cancer cells, where it may have both tumor-suppressing and tumor-promoting roles. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (3) differs in the 5' UTR, lacks an in-frame portion of the 5' coding region, and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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