Relb (NM_001290457) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	shep
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228286 representing NM_001290457 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGAGTCGCCGCGCTGCCAGAGAGTCCGCGCCCGAGCTAGGGGCCTTGGGTTCCAGTGACCTCTCTT CCCTGTCACTAACGGTCTCCAGGACCACAGAAATCATCGACGAATACATTAAGGAGAACGGCTTTGGCCT GGACGGGACACAGCTGAGTGAGATGCCGCGCCTGGTGCCCCGCGGGCCCGCCTCACTGAGCAGCGTCACG CTGGGCCCTGCTGCACCACCGCCTCCGGCCACGCCGTCCTGGAGCTGCACACTGGGCAGGCTGGTGTCAC CCGGCCCGTGCCCACGGCCGTACCTGGTCATCACAGAGCAGCCAAAGCAGCGTGGCATGCGCTTCCGCTA CGAGTGCGAGGGCCGCTCGGCCGGCAGCATCCTCGGGGAGAGCAGCACCGAAGCCAGCAAGACCCTGCCC GCCATCGAGCTTCGAGACTGTGGCGGGCTGCGGGAGGTGGAGGTGACGGCGTGCCTGGTGTGGAAGGACT GGCCACACCGGGTACACCCACATAGCCTCGTGGGGAAAGACTGCACGGACGGCGTCTGCAGGGTGCGGCT GCGGCCTCACGTCAGCCCCCGGCACAGCTTTAACAACCTGGGCATCCAGTGTGTTAGGAAGAAGGAAATT GAAGCTGCCATTGAGCGGAAGATCCAGCTGGGAATTGACCCCTACAATGCTGGCTCCCTGAAGAACCATC AGGAGGTCGACATGAATGTCGTCAGGATCTGCTTCCAGGCCTCCTATCGGGACCAGCAGGGACATCTGCA CCGCATGGACCCCATCCTCTCTGAGCCTGTCTACGACAAGAAGTCCACCAACACATCGGAGCTGCGGATT TGCCGAATCAACAAGGAGAGCGGGCCGTGCACAGGTGGTGAGGAGCTGTACTTGCTCTGTGACAAGGTGC AAAAAGAGGACATATCCGTGGTGTTCAGCACAGCTTCCTGGGAAGGCCGTGCCGACTTCTCTCAAGCTGA TGTGCACCGGCAGATCGCCATTGTGTTCAAAACGCCACCCTACGAGGACCTGGAGATCTCAGAGCCCGTG ACTGTCAATGTGTTCTTGCAGCGGCTCACGGATGGGGTGTGCAGCGAGCCGCTGCCCTTCACGTACCTGC CTCGGGATCATGACAGCTACGGTGTGGACAAGAAGCGAAAGCGGGGACTGCCTGATGTCCTTGGAGAGTT GAGCAGCTCTGATCCACATGGAATCGAGAGCAAACGAAGGAAAAAGAAACCAGTGTTCTTGGACCACTTC CTGCCTGGCCACAGCTCAGGCCTGTTCCTCCCACCATCGGCTCTGCAGCCGGCAGACTCTGATTTCTTCC CTGCTTCCATATCCCTTCCTGGGCTGGAGCCTCCTGGTGGACCCGATCTCCTGGACGATGGCTTTGCCTA TGATCCTTCTGCCCCCACGCTCTTCACTATGTTGGACCTGCTGCCCCCAGCACCACCACTTGCCAGTGCT GTGGTGGGTAGCGGGGGTGCAGGGGCCACCGTTGTGGAGTCTTCTGGCCCAGAGCCCCTATCACTGGACT CTTTTGCAGCGCCGGGCCCCGGGGATGTTGGTACTGCTAGCCTTGTGGGCAGCAACATGTTTCCCAACCA GTACCGAGAGGCAGCTTTCGGGGGTGGCCTCCTATCTCCAGGGCCTGAAGCCACGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290457
Insert Size	1668 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290457.1, NP_001277386.1
RefSeq Size	2194 bp
RefSeq ORF	1668 bp
Locus ID	19698
UniProt ID	Q04863
Gene Summary	NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49 (By similarity). As a member of the NUPR1/RELB/IER3 survival pathway, may allow the development of pancreatic intraepithelial neoplasias. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer in a CRY1/CRY2 independent manner. Increased repression of the heterodimer is seen in the presence of NFKB2/p52. Is required for both T and B lymphocyte maturation and function (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.

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