Nrxn3 (NM_001252074) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228330 representing NM_001252074 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCACCTGAGAATCCACCCAAGACGGAGCCCTCCTCGCCGGCCGGCCTGGACGCTTGGGATCTGGTCCC TCTTCTGGGGATGTATCGTCAGCTCTGTGTGGAGTTCTTCTAATGTAGCCTCCTCCTCCTCTTCCTCTCC GGGATCTCACTCTCAGCAGGAACACCATTTCCACGGCAGCAAGCACCACTCTGTGCCTATTTCTATCTAT CGCTCCCCTGTTTCCCTTCGAGGAGGGCACGCTGGTGCAACATACATCTTTGGGAAAAGCGGTGGCCTCA TCCTCTATACCTGGCCAGCAAATGACAGACCCAGCACACGCTCTGACCGTCTCGCCGTGGGCTTCAGCAC TACTGTGAAGGATGGTATCTTAGTACGCATTGACAGCGCCCCTGGACTTGGCGACTTCCTCCAGCTTCAC ATAGAACAAGGGAAAATTGGAGTTGTCTTCAATATTGGCACAGTTGACATCTCCATCAAAGAAGAGAGAA CTCCTGTCAATGATGGCAAATACCACGTTGTGCGCTTCACCAGGAATGGGGGAAATGCTACACTTCAGGT GGACAACTGGCCAGTGAATGAGCACTATCCTACAGGCAACACTGATAATGAACGCCTCCAAATGGTAAAA CAGAAAATCCCCTTCAAATATAACCGGCCCGTAGAGGAGTGGCTGCAGGAAAAAGGCCGGCAGCTAACCA TCTTCAACACCCAGGCGCAAATAGCCATCGGAGGAAAGGACAAAGGACGTCTCTTCCAAGGTCAACTCTC TGGGCTCTATTATGATGGTTTGAAAGTACTGAACATGGCAGCTGAGAACAACCCTAATATTAAAATCAAT GGAAGTGTCCGGCTAGTGGGAGAAGTCCCATCAGTCTCAGGAACAACACAGACAACGTCCATGCCACCTG AAATGTCTACCACCGTCATGGAAACCACCACCACCATGGCTACGACCACCACCCGAAAGAACCGCTCTAC AGCCAGCATTCAGCCCACGTCAGATGATCTTGTTTCATCTGCTGAATGTTCAAGTGATGATGAAGACTTT GTCGAATGTGAACCAAGTACAGGTAGGTCAGATAAGAGTCTTTCCACTTCAATCTTCGAAGGTGGCTACA AAGCACATGCGCCCAAGTGGGAATCCAAGGACTTTAGACCTAACAAAGTCTCGGAAACTAGTAGAACTAC AACCACCTCTTTGTCCCCTGAGCTGATCCGCTTCACAGCGTCCTCCTCGTCTGGGATGGTGCCCAAATTG CCAGCTGGCAAAATGAATAACCGTGATCTCAAACCCCAGCCTGATATAGTCTTGCTTCCGTTGCCCACTG CCTATGAGCTAGACAGCACCAAACTGAAGAGCCCACTAATTACTTCCCCCATGTTCCGTAATGTGCCCAC AGCAAACCCCACGGAGCCAGGAATCAGACGGGTTCCGGGGGCCTCAGAGGTGATCCGGGAGTCCAGCAGT ACAACAGGGATGGTCGTCGGCATTGTGGCTGCTGCCGCCCTCTGCATCTTGATCCTCCTGTACGCCATGT ACAAGTACAGGAACAGGGACGAGGGGTCCTATCAAGTGGACGAGACGAGGAACTACATCAGCAACTCGGC CCAGAGCAACGGCACGCTCATGAAGGAGAAGCAAGCCAGCTCCAAGAGCGGCCATAAGAAACAGAAAAAC AAGGACAAGGAGTATTATGTGTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252074
Insert Size	1704 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252074.2, NP_001239003.1
RefSeq Size	5157 bp
RefSeq ORF	1704 bp
Locus ID	18191
UniProt ID	Q8C985
Gene Summary	This gene encodes a member of a family of proteins that function in the nervous system as receptors and cell adhesion molecules. Extensive alternative splicing and the use of alternative promoters results in multiple transcript variants for this gene, but the full-length nature of many of these variants has not been determined. Transcripts that initiate from an upstream promoter encode alpha isoforms, which contain epidermal growth factor-like (EGF-like) sequences and laminin G domains. Transcripts initiating from the downstream promoter encode beta isoforms, which lack EGF-like sequences. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (3) differs in the 5' UTR and contains multiple differences in the coding region, including the lack of multiple 5' exons, compared to variant 1. It initiates translation at an alternate start codon. The encoded isoform (3) is shorter and has a distinct N-terminus, compared to isoform 1. This variant encodes a beta isoform of neurexin 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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