Orc2 (NM_001271526) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	AU041563; Orc2l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228353 representing NM_001271526 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCACTCTGCAGTTAAAGGAAACCAAGGTGCCAAGCGTGCAGTTTGTGGGAGATGATGATGTTCTTA GTCACATTCTGGACAGAGAAGGAGGAACTAAATTAAAGAAGGAGAAAGCTCAGCTTTTGGTCAATCCCCA GAAAGTAATAAAGAAGGCAGACTGTGAGTTGGAGAAAAGTGACCTGGAAGTTTTAGAGGATCAGAACTAC GTGAAAGTCTTGGGAAGAAATATTCAAGAATCCTTGGGAAACGGCTCTGCTAAAGATGGTAGGAATAAAG TTTATTCTTTTCAACAAAGAAAGCACCCTGAAGAGATGACTAAATTGGCTTTAGAACTAGCAAAAACATC AGGAAAAAAGGATCCACTTGACTCAAATGATCCTGAAATCACAAAAAACATTGCTCAAAAAAGTAAAGGG CATTCTACTTCAGAGAAAGCTCCACTGGTGAACAATAACAAAACTGAATTTCTGTCAACACAACCTCATA ATCTAAGGAAAAGAATAATAGCGTCAAGGTCTCATTATGACAGTGAGAGTGAGTATTCTGCTTCCAGCTC AGAGGATGATGAAGAGGCTACAAAAGACGAAGAAGAAGACACTAATGTGGCCAGGCTCTCTCAGAAGAGT CAAGGTCAGAATAGACTTCTTCCAGCTCCTGTTTCCAAAGAGACACTGCCTAAGAAAAAGAAAAGAGACA AAGCGAGTGACTTGGTAGAAGAATATTTTGAAGCCCATAGTAGTTCAAAAGTCTTAACATCTGATCGAAC GCTGCAGAGGCTGAGGAGAGCCAGAGTGGATCAGAAAACTCTGCATAACTTACTGAGAAAATTTGTGCCT TCCTTTTCTGCTGAAATTGAACGATTGAATCAACAGCATGAGAAACTGTTTCATAAATGGATGCTTCAGT TACACCTTGGGTTCAACATTGTGCTTTATGGCTTGGGGTCCAAGAGAGATTTACTAGAAAAGTTTCGAAC CACCATGCTTCAAGATTCCATTCATGTTGTCATCAACGGCTACTTTCCTGGCGTCAGTGTGAAATCAATC CTGAACTCTATAACTGAAGATGTCCTCAGTCATGTGGGTACTTTCCAAAGTGTTCTGGATCAGCGGGACT GGATAATAAACAGATTTAAAGAAGATTCTTCTTTAGAACTCTTCCTTCTTATCCACAATTTGGATAGCCA AATGTTAAGAGGAGACAATAGCCAGCAAATTCTTGGACAGTTGTCATCATTACATAACGTGTATCTCATA GCATCTATTGACCATCTCAATGCTCCTCTCATGTGGGATCATGCAAAGCAGAGTCTTTATAACTGGCTTT GGTATGAAACTACTACATACAGTCCTTATACTGAAGAAACCTCCTATGAGAACTCCCTTCTGGTTAAGCA GTCTGGATCTCTGCCACTTAGTTCTCTGATTCATGTCTTACGAAGCCTTACCCCCAATGCAAGGGGGATT TTCAGGCTACTTATGAAGTTCCAGCTGGATAACCAGGACAGCCCTTCCTACATTGGACTTTCTTTTCAAG ATTTTTACCAGCAGTGTAGGGAAGCATTCCTTGTTAACAGTGATCTCACACTCCGAGCCCAGTTAACTGA ATTTAGGGACCACAAACTTATAAGAACAAAGAAGGGAACTGATGGTGTAGAATATTTATTAATTCCTGTT GACAGCGGAATATTGGCTGATTTCTTGGAGAAGGAAGAGGAGGAGGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271526
Insert Size	1731 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001271526.1, NP_001258455.1
RefSeq Size	3555 bp
RefSeq ORF	1731 bp
Locus ID	18393
UniProt ID	Q60862
Gene Summary	Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication (By similarity). Binds histone H3 and H4 trimethylation marks H3K9me3, H3K20me3 and H4K27me3. Stabilizes LRWD1, by protecting it from ubiquitin-mediated proteasomal degradation. Also stabilizes ORC3 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (c) uses an alternate splice site in the 5' UTR compared to variant a. Both variants a and c encode the same isoform (A). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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