Picalm (NM_001252522) Mouse Untagged Clone

CAT#: MC228459

Picalm (untagged) - Mouse phosphatidylinositol binding clathrin assembly protein (Picalm), transcript variant 4



  "NM_001252522" in other vectors (1)

CNY 5,990.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms CALM; CLTH; fit-1; fit1; mKIAA4114; PAP180
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228459 representing NM_001252522
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCTGGCCAGAGCCTGACGGACCGAATCACCGCGGCCCAGCACAGTGTCACTGGCTCCGCGGTATCTA
AGACAGTATGCAAGGCCACGACCCACGAGATCATGGGCCCCAAGAAAAAGCACCTGGACTACTTAATTCA
GTGTACAAATGAGATGAATGTGAACATCCCACAGTTGGCGGACAGTTTGTTTGAAAGAACTACTAACAGT
AGTTGGGTGGTGGTCTTCAAATCACTCATTACAACTCATCATTTGATGGTGTATGGAAATGAGCGTTTCA
TTCAGTATTTGGCTTCAAGAAACACATTGTTTAACTTAAGCAATTTTTTGGATAAAAGTGGATTGCAAGG
ATATGATATGTCTACATTTATTAGACGGTATAGTAGATACTTAAATGAAAAAGCAGTTTCATACAGACAA
GTTGCATTCGATTTCACAAAAGTGAAGAGAGGAGCTGATGGAGTTATGAGAACAATGAACACAGAAAAAC
TCTTAAAAACTGTACCAATTATCCAAAATCAAATGGATGCACTTCTTGATTTTAATGTTAATAGTAATGA
ACTTACAAATGGGGTAATAAATGCTGCCTTCATGCTCCTCTTCAAAGATGCCATTAGACTATTTGCAGCA
TACAATGAAGGAATTATTAATTTATTGGAAAAATATTTTGATATGAAAAAGAACCAGTGCAAAGAAGGTC
TTGACATCTATAAGAAGTTTCTGACTAGGATGACAAGAATCTCAGAGTTTCTGAAAGTTGCAGAGCAAGT
TGGAATTGACAGAGGAGATATTCCAGATCTTTCACAGGCCCCCAGCAGTCTTCTTGATGCTTTAGAACAA
CATTTAGCTTCCTTGGAAGGAAAGAAAATCAAAGATTCCACAGCTGCAAGCAGGGCTACAACACTTTCCA
ATGCAGTCTCTTCTTTGGCAAGCACTGGCCTATCTCTGACCAAAGTGGATGAAAGGGAAAAACAGGCAGC
ATTAGAGGAAGAACAGGCTCGTTTAAAAGCATTAAAGGAACAGCGTCTAAAAGAACTTGCAAAGAAACCG
CATACCTCTCTAACAACTGCAGCCTCTCCGGTGTCCACCTCAGCAGGGGGAATAATGACTGCACCAGCCA
TTGACATATTTTCTACCCCTAGTTCTTCTAACAGCACATCTAAGCTGCCAAATGACCTGCTTGACTTGCA
GCAGCCAACCTTTCACCCGTCTGTCCACGCCATGTCGGCTGCTCCTCAGGGAGCGAGTACATGGGGAGGA
TTCAGTCCTTCTCCAGTTGCACAGCCACATTCTTCAGCTGGCCTTAATGTTGACTTTGAATCTGTGTTTG
GAAATAAATCTACGAATGTTGCTGTAGATTCTGGTGGCTTTGATGAACTAGGTGGACTTCTCAAACCAAC
AGTGGCCTCCCAGAACCAGAGTCTTCCTGTTGCCAAACTTCCGCCTAACAAATTAGTGTCTGACGACTTG
GATTCATCTTTAGCCAACCTTGTGGGCAATCTTGGCATTGGAAATGGAACCACTAAGAATGATGTAAGTT
GGAGTCAACCAGGTGAAAAGAAGTTAACTGGAGGATCTAACTGGCAACCAAAGGTTGCACCAACAACTGC
CTGGAGCGCTGCAACAATGAATGGCATGCATTTTCCACAATACGCGCCCCCTGTAATGGCCTATCCTGCT
ACTACACCAACGGGCATGATAGGATATGGAATTCCTCCTCAGATGGGAAGTGTACCTGTAATGACACAAC
CAACCTTAATATACAGCCAGCCTGTCATGAGACCACCAAACCCCTTTGGCCCTGTATCAGGAGCACAGAT
ACAGTTTATGTAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001252522
Insert Size 1833 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001252522.1, NP_001239451.1
RefSeq Size 4024 bp
RefSeq ORF 1833 bp
Locus ID 233489
UniProt ID Q7M6Y3
Gene Summary Cytoplasmic adapter protein that plays a critical role in clathrin-mediated endocytosis which is important in processes such as internalization of cell receptors, synaptic transmission or removal of apoptotic cells. Recruits AP-2 and attaches clathrin triskelions to the cytoplasmic side of plasma membrane leading to clathrin-coated vesicles (CCVs) assembly. Furthermore, regulates clathrin-coated vesicle size and maturation by directly sensing and driving membrane curvature. In addition to binding to clathrin, mediates the endocytosis of small R-SNARES (Soluble NSF Attachment Protein REceptors) between plasma membranes and endosomes including VAMP2, VAMP3, VAMP4, VAMP7 or VAMP8. In turn, PICALM-dependent SNARE endocytosis is required for the formation and maturation of autophagic precursors. Modulates thereby autophagy and the turnover of autophagy substrates such as MAPT/TAU or amyloid precursor protein cleaved C-terminal fragment (APP-CTF).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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