Grip1 (NM_001277295) Mouse Untagged Clone

CAT#: MC228533

Grip1 (untagged) - Mouse glutamate receptor interacting protein 1 (Grip1), transcript variant 7



  "NM_001277295" in other vectors (1)

CNY 6,180.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 4931400F03Rik; eb; GRIP
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228533 representing NM_001277295
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACAGCAAAACGAGCTGAAAGGAAGGAGATGAAGAGACCCAATAGTTTTCACCTTCCTTTTCGTCCAT
CTTTAAGAAAAGGACAGAAGAAAAACGCAGCTCATGTGTCTTTAGCCTCCAGCACTGTGGGGTTGGCTGG
CCAGGTCGTTCACACTGAAACCACAGAGGTTGTGCTGACGGCTGACCCTGTCACGGGCTTCGGAATCCAA
CTGCAGGGCAGCGTGTTTGCCACAGAGACGCTCTCCTCTCCGCCTCTGATTTCCTATATTGAAGCTGACA
GCCCAGCAGAGAGATGTGGTGTGCTACAGATTGGAGACAGAGTCATGGCCATTAATGGAATCCCAACAGA
AGACAGCACCTTCGAGGAAGCCAATCAACTCCTGAGAGACTCTTCCATCACGAGCAAAGTCACACTAGAA
ATCGAGTTTGATGTTGCAGAGTCTGTCATCCCAAGTAGTGGAACATTTCATGTAAAACTGCCCAAGAAGC
ACAGCGTGGAACTTGGAATAACCATCAGTTCGCCATCCAGTAGAAAACCGGGGGACCCCCTTGTCATTTC
AGATATCAAGAAAGGCAGTGTGGCACACAGAACCGGAACTCTGGAACTGGGAGACAAACTGCTTGCGATA
GATAACATCCGGCTGGATAACTGTTCCATGGAAGATGCGGTCCAGATCCTCCAGCAGTGTGAAGACCTGG
TGAAGCTCAAAATCCGCAAAGATGAAGATAACTCAGACGAGCAAGAGAGTTCCGGAGCGATTATTTACAC
GGTGGAGCTGAAGCGCTATGGGGGGCCCCTTGGCATCACAATTTCTGGAACTGAAGAGCCGTTTGATCCT
ATTATCATCTCGAGCCTCACTAAAGGGGGATTAGCTGAAAGGACTGGAGCGATCCACATCGGAGATAGAA
TCCTAGCCATCAATAGCAGCAGCTTGAAGGGGAAGCCTCTGAGTGAAGCCATCCACTTGCTCCAGATGGC
AGGAGAGACTGTCACCCTGAAAATTAAGAAACAGACAGATGGCTCAACTTTTCAGACTTCAGGATACAAT
TACAACACCTATGATTGGAGGAGTCCAAAGCAAAGAACCAGCCTGTCCCCAGTCCCCAAGCCTCGAAGCC
AGACGTACCCAGATGTGGGCCTGAGTAATGAAGACTGGGATCGATCCACAGCCAGTGGCTTTGTAGGGGC
TTCTGACAGTGCAGATGCTGAACAAGAGGAAAACTTCTGGTCTCAAGCATTGGAGGACCTGGAGACCTGC
GGCCAGTCGGGGATCCTGAGAGAGCTTGAGGCAACAATCATGTCGGGGAGTACTATGAGTTTGAATCATG
AGGCTCCAATGGCTCGCAGTCAGCTGGGGCGACAGGCCAGCTTCCAGGAACGGAGCAGTTCACGGCCACA
CTATAGCCAAACAACTCGCAGCAACACCCTGCCCTCAGACGTGGGCAGAAAGTCTGTAACCCTGCGGAAA
ATGAAGCAAGAAATAAAGGAGATCATGTCCCCAACTCCGGTGGAGCTACACAAGGTGACCTTATACAAGG
ACTCTGGCATGGAGGACTTCGGGTTCAGTGTGGCAGATGGCCTGCTGGAGAAAGGCGTGTATGTCAAAAA
TATCCGCCCAGCTGGGCCAGGTGATGTTGGGGGCTTGAAGCCCTACGACAGGCTCTTACAGGTAAATCAC
GTGCGGACGAGAGACTTTGACTGCTGCCTGGTGGTGCCTCTCATAGCTGAATCTGGCAACAAGCTGGACC
TGGTTATTAGCAGAAATCCACTGGCCTCCCAGAAGTCGATAGAACAGCCGGCTCTGCCCAGCGACTGGAG
CGAACAGAACAGCGCTTTCTTCCAGCAACCCAGCCACGGTGGTAATCTAGAGACACGAGAACCCACTAAC
ACACTATAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001277295
Insert Size 1899 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001277295.1, NP_001264224.1
RefSeq Size 3758 bp
RefSeq ORF 1899 bp
Locus ID 74053
UniProt ID Q925T6
Gene Summary This gene encodes a protein containing multiple PDZ (post synaptic density protein, Drosophila disc large tumor suppressor, and zonula occludens-1 protein) domains. The encoded protein acts as a mediator between cytoskeletal and membrane proteins, particularly in neuronal cells, and facilitates complex formation at the cell membrane. Mutation of this gene can cause embryonic lethality resulting from defects of the dermo-epidermal junction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2013]
Transcript Variant: This variant (7) contains multiple differences compared to variant 1, including the lack of multiple exons. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (7, also known as tau) is shorter and has a distinct N-terminus, compared to isoform 1. This isoform is localized to the nucleus (PMID: 15507123). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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