Pomgnt1 (NM_001290658) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	0610016I07Rik; 4930467B06Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228544 representing NM_001290658 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGACCAAGGAAGTCAAGGACTCAAATGGGAAAATACAAGACCACGGGACAGGGGCTGTGCTTT TCCTGCTGGTGACCGTCATTGTCAACATCAAGTTGATCCTGGACACTCGGAGAGCAATCAGCGAGGCCAA TGAAGACCCAGAACCAGAGCAAGACTATGATGAGGCCCTAGGACGTCTGGAGTCCCCTCGGCGCAGAGGC AGCAGTCCCCGACGGGTGCTGGATGTGGAAGTATACTCCAGTAGAAGCAAAGTGTATGTGGCTGTGGATG GCACCACGGTGCTCGAGGATGAAGCCCGGGAGCAGGGCCGAGGCATCCATGTCATAGTCCTCAACCAGGC CACGGGCCACGTGATGGCAAAGCGCGTGTTTGACACATACTCTCCTCATGAGGATGAGGCCATGGTGCTG TTCCTCAACATGGTGGCCCCCGGCCGCGTGCTGATCTGCACGGTGAAGGATGAGGGTTCCTTCCACCTCA AGGACACAGCAAAGGCTCTGCTGAGGAGCCTGGGAAGTCAGGCAGGCCCTGCCCTTGGCTGGAGGGACAC CTGGGCCTTCGTGGGACGAAAAGGAGGTCCTGTACTTGGCGAGAAACATTCTAAATCTCCTGCCCTCTCC TCCTGGGGTGACCCAGTCCTGCTGAAGACAGATGTGCCACTGAGCTCAGCTGAAGAGGCTGAGTGTCACT GGGCAGATACGGAGTTGAACCGCCGCCGCAGGCGCTTCTGCAGCAAAGTGGAGGGCTACGGGAGCGTGTG CAGCTGCAAGGACCCGACGCCTATTGAGTTCAGCCCTGACCCACTTCCAGACAATAAGGTCCTCAATGTG CCTGTGGCTGTCATTGCAGGGAACCGGCCCAATTACCTGTACAGGATGCTGCGCTCTCTGCTCTCAGCCC AAGGGGTATCTCCACAGATGATAACGGTCTTCATTGATGGCTACTATGAGGAGCCAATGGATGTGGTGGC GCTGTTTGGTCTGAGAGGCATCCAGCATACTCCTATCAGCATCAAGAACGCCCGTGTGTCTCAGCACTAC AAGGCCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGTTTGCTGTGGTTCTGGAAGAGGACC TGGACATTGCCGTGGATTTTTTCAGTTTCCTGAGCCAGTCCATCCACCTGCTGGAGGAAGATGACAGCCT GTACTGCATCTCTGCCTGGAATGACCAGGGGTATGAACACACAGCTGAGGATCCAGCACTACTGTACCGT GTGGAGACCATGCCTGGGCTAGGATGGGTGCTCAGGAAATCTTTATACAAGGAGGAGCTTGAGCCCAAGT GGCCCACACCAGAAAAGCTGTGGGATTGGGACATGTGGATGCGGATGCCGGAACAGCGCCGGGGCCGAGA GTGTATCATCCCGGATGTTTCCCGATCCTACCACTTTGGCATCGTCGGCCTCAACATGAACGGCTACTTT CATGAGGCCTACTTCAAGAAGCACAAGTTCAACACAGTTCCAGGTGTCCAGCTCAGGAATGTAGACAGTC TGAAGAAAGAAGCTTATGAAGTGGAAATTCACAGACTGCTCAGTGAGGCCGAGGTTTTGGACCACAGCAA GGACCCTTGTGAAGACTCCTTCCTGCCAGACACGGAGGGCCACACCTATGTGGCGTTTATCCGAATGGAG ACAGATGATGACTTCGCCACCTGGACTCAGCTTGCCAAGTGCCTCCATATTTGGGACCTGGATGTTCGTG GCAACCACCGGGGCCTGTGGAGATTATTTCGGAAGAAGAACCACTTTTTGGTGGTGGGGGTCCCAGCCTC CCCTTACTCAGTGAAGAAGCCACCATCAGTCACCCCTATTTTCCTGGAGCCACCCCCAAAGGAAGAAGGA GCCCCAGGGGCTGCTGAACAAACGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001290658
Insert Size	1917 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290658.1, NP_001277587.1
RefSeq Size	2650 bp
RefSeq ORF	1917 bp
Locus ID	68273
UniProt ID	Q91X88
Gene Summary	Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) contains an alternate 5' terminal exon and initiates translation at an alternate start codon, compared to variant 1. It encodes isoform 3, which has a shorter and distinct N-terminus, compared to isoform 1.

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