Pomgnt1 (NM_001290658) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	0610016I07Rik; 4930467B06Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230755 representing NM_001290658 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGGGACCAAGGAAGTCAAGGACTCAAATGGGAAAATACAAGACCACGGGACAGGGGCTGTGCTTT TCCTGCTGGTGACCGTCATTGTCAACATCAAGTTGATCCTGGACACTCGGAGAGCAATCAGCGAGGCCAA TGAAGACCCAGAACCAGAGCAAGACTATGATGAGGCCCTAGGACGTCTGGAGTCCCCTCGGCGCAGAGGC AGCAGTCCCCGACGGGTGCTGGATGTGGAAGTATACTCCAGTAGAAGCAAAGTGTATGTGGCTGTGGATG GCACCACGGTGCTCGAGGATGAAGCCCGGGAGCAGGGCCGAGGCATCCATGTCATAGTCCTCAACCAGGC CACGGGCCACGTGATGGCAAAGCGCGTGTTTGACACATACTCTCCTCATGAGGATGAGGCCATGGTGCTG TTCCTCAACATGGTGGCCCCCGGCCGCGTGCTGATCTGCACGGTGAAGGATGAGGGTTCCTTCCACCTCA AGGACACAGCAAAGGCTCTGCTGAGGAGCCTGGGAAGTCAGGCAGGCCCTGCCCTTGGCTGGAGGGACAC CTGGGCCTTCGTGGGACGAAAAGGAGGTCCTGTACTTGGCGAGAAACATTCTAAATCTCCTGCCCTCTCC TCCTGGGGTGACCCAGTCCTGCTGAAGACAGATGTGCCACTGAGCTCAGCTGAAGAGGCTGAGTGTCACT GGGCAGATACGGAGTTGAACCGCCGCCGCAGGCGCTTCTGCAGCAAAGTGGAGGGCTACGGGAGCGTGTG CAGCTGCAAGGACCCGACGCCTATTGAGTTCAGCCCTGACCCACTTCCAGACAATAAGGTCCTCAATGTG CCTGTGGCTGTCATTGCAGGGAACCGGCCCAATTACCTGTACAGGATGCTGCGCTCTCTGCTCTCAGCCC AAGGGGTATCTCCACAGATGATAACGGTCTTCATTGATGGCTACTATGAGGAGCCAATGGATGTGGTGGC GCTGTTTGGTCTGAGAGGCATCCAGCATACTCCTATCAGCATCAAGAACGCCCGTGTGTCTCAGCACTAC AAGGCCAGCCTCACTGCCACTTTCAACCTGTTTCCGGAGGCCAAGTTTGCTGTGGTTCTGGAAGAGGACC TGGACATTGCCGTGGATTTTTTCAGTTTCCTGAGCCAGTCCATCCACCTGCTGGAGGAAGATGACAGCCT GTACTGCATCTCTGCCTGGAATGACCAGGGGTATGAACACACAGCTGAGGATCCAGCACTACTGTACCGT GTGGAGACCATGCCTGGGCTAGGATGGGTGCTCAGGAAATCTTTATACAAGGAGGAGCTTGAGCCCAAGT GGCCCACACCAGAAAAGCTGTGGGATTGGGACATGTGGATGCGGATGCCGGAACAGCGCCGGGGCCGAGA GTGTATCATCCCGGATGTTTCCCGATCCTACCACTTTGGCATCGTCGGCCTCAACATGAACGGCTACTTT CATGAGGCCTACTTCAAGAAGCACAAGTTCAACACAGTTCCAGGTGTCCAGCTCAGGAATGTAGACAGTC TGAAGAAAGAAGCTTATGAAGTGGAAATTCACAGACTGCTCAGTGAGGCCGAGGTTTTGGACCACAGCAA GGACCCTTGTGAAGACTCCTTCCTGCCAGACACGGAGGGCCACACCTATGTGGCGTTTATCCGAATGGAG ACAGATGATGACTTCGCCACCTGGACTCAGCTTGCCAAGTGCCTCCATATTTGGGACCTGGATGTTCGTG GCAACCACCGGGGCCTGTGGAGATTATTTCGGAAGAAGAACCACTTTTTGGTGGTGGGGGTCCCAGCCTC CCCTTACTCAGTGAAGAAGCCACCATCAGTCACCCCTATTTTCCTGGAGCCACCCCCAAAGGAAGAAGGA GCCCCAGGGGCTGCTGAACAAACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230755 representing NM_001290658 Red=Cloning site Green=Tags(s) MEGTKEVKDSNGKIQDHGTGAVLFLLVTVIVNIKLILDTRRAISEANEDPEPEQDYDEALGRLESPRRRG SSPRRVLDVEVYSSRSKVYVAVDGTTVLEDEAREQGRGIHVIVLNQATGHVMAKRVFDTYSPHEDEAMVL FLNMVAPGRVLICTVKDEGSFHLKDTAKALLRSLGSQAGPALGWRDTWAFVGRKGGPVLGEKHSKSPALS SWGDPVLLKTDVPLSSAEEAECHWADTELNRRRRRFCSKVEGYGSVCSCKDPTPIEFSPDPLPDNKVLNV PVAVIAGNRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMDVVALFGLRGIQHTPISIKNARVSQHY KASLTATFNLFPEAKFAVVLEEDLDIAVDFFSFLSQSIHLLEEDDSLYCISAWNDQGYEHTAEDPALLYR VETMPGLGWVLRKSLYKEELEPKWPTPEKLWDWDMWMRMPEQRRGRECIIPDVSRSYHFGIVGLNMNGYF HEAYFKKHKFNTVPGVQLRNVDSLKKEAYEVEIHRLLSEAEVLDHSKDPCEDSFLPDTEGHTYVAFIRME TDDDFATWTQLAKCLHIWDLDVRGNHRGLWRLFRKKNHFLVVGVPASPYSVKKPPSVTPIFLEPPPKEEG APGAAEQT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290658
ORF Size	1914 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290658.1, NP_001277587.1
RefSeq Size	2650 bp
RefSeq ORF	1917 bp
Locus ID	68273
UniProt ID	Q91X88
MW	72.5 kDa
Gene Summary	Participates in O-mannosyl glycosylation by catalyzing the addition of N-acetylglucosamine to O-linked mannose on glycoproteins. Catalyzes the synthesis of the GlcNAc(beta1-2)Man(alpha1-)O-Ser/Thr moiety on alpha-dystroglycan and other O-mannosylated proteins, providing the necessary basis for the addition of further carbohydrate moieties. Is specific for alpha linked terminal mannose.[UniProtKB/Swiss-Prot Function]

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