Acox1 (NM_001271898) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	A; Acox; AOX; D130055E20Rik; Paox
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228602 representing NM_001271898 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATCCCGATCTGCGCAAGGAGCGGGCCGCCGCCACCTTCAATCCAGAGTTAATCACGCACATCTTGG ATGGTAGTCCGGAGAACACCCGGCGCCGTCGAGAAATCGAGAACTTGATTCTGAACGATCCAGACTTCCA ACATGAGGACTATAACTTCCTCACTCGAAGCCAGCGTTACGAGGTGGCTGTTAAGAAGAGTGCCACCATG GTGAAGAAGATGAGGGAATTTGGCATCGCAGACCCTGAAGAAATCATGTGGTTTAAAAAACTACATATGG TCAATTTTGTGGAACCTGTTGGCCTCAATTACTCCATGTTTATCCCTACCTTGCTGAATCAGGGCACCAC TGCTCAGCAGGAGAAATGGATGCACCCGTCCCAAGAACTCCAGATAATTGGCACCTACGCCCAGACGGAG ATGGGCCACGGAACTCATCTTCGAGGCTTGGAAACCACTGCCACATATGACCCCAAGACCCAAGAGTTCA TTCTCAACAGCCCAACTGTGACTTCCATCAAGTGGTGGCCTGGGGGGCTTGGGAAGACTTCCAATCATGC GATAGTCCTGGCTCAGCTCATCACTCGAGGGGAGTGCTACGGGTTACATGCCTTTGTTGTCCCTATCCGT GAGATTGGGACCCACAAGCCTCTGCCAGGCATCACTGTTGGGGATATCGGCCCCAAGTTTGGTTATGAAG AGATGGATAATGGCTACCTGAAGATGGACAATTACCGTATTCCTAGAGAGAACATGTTGATGAAATATGC CCAGGTGAAGCCTGACGGCACGTATGTAAAACCTCTGAGTAACAAGCTGACATATGGGACCATGGTTTTC GTAAGGTCCTTCCTCGTGGGAAGTGCAGCTCAGAGTCTGTCCAAGGCATGCACCATTGCCATTCGATACA GTGCTGTGAGGCGCCAGTCTGAAATCAAGAGAAGCGAGCCAGAGCCCCAGATTTTGGATTTTCAGACGCA GCAGTATAAACTCTTCCCGCTCCTGGCCACCGCCTATGCCTTCCACTTTCTCGGAAGATACATAAAGGAG ACCTACATGCGGATTAATGAGAGCATTGGCCAAGGCGACCTGAGTGAGCTGCCTGAGCTTCATGCCCTCA CAGCTGGGCTGAAGGCTTTTACTACCTGGACAGCCAATGCTGGTATCGAAGAATGTCGGATGGCTTGCGG TGGGCACGGCTATTCTCACAGCAGTGGGATTCCAAATATTTACGTCACGTTTACCCCGGCCTGCACCTTC GAGGGGGAGAACACTGTTATGATGCTGCAGACGGCCAGGTTCTTGATGAAAATCTATGACCAGGTTCAGT CGGGGAAGCTGGTGGGTGGTATGGTGTCGTACTTGAATGACCTGCCGAGCCAGCGTATCCAGCCGCAGCA GGTGGCAGTCTGGCCAACTCTGGTGGACATTAACAGCCTGGACAGCCTGACAGAAGCCTACAAGCTACGT GCAGCCAGATTGGTAGAAATTGCTGCAAAAAACCTTCAGGCCCAAGTGAGTCACAGGAAGAGCAAGGAAG TGGCGTGGAACTTGACTTCTGTCGACCTTGTTCGCGCAAGTGAGGCGCACTGCCACTACGTGACCGTTAA GGTCTTTGCAGATAAACTCCCCAAGATTCAAGACAGAGCCGTGCAAGCCGTGCTGAGGAACCTGTGTCTC TTATATTCTCTCTATGGGATCAGCCAGAAAGGAGGGGATTTTCTTGAGGGGAACATCATCACAGGGGCTC AGATGTCACAGGTAAACAGTCGGATCCTGGAGCTGCTCACAGTGACTCGCCCCAACGCTGTGGCTTTGGT GGATGCCTTTGACTTTAAGGATGTGACCCTTGGCTCTGTTCTCGGCCGCTATGATGGCAATGTGTATGAA AACTTGTTTGAGTGGGCCAAGAAGTCCCCACTGAACAAGACAGAGGTCCACGAATCTTACTACAAGCACT TGAAGCCCCTGCAGTCGAAGCTTTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001271898
Insert Size	1986 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271898.1, NP_001258827.1
RefSeq Size	3992 bp
RefSeq ORF	1986 bp
Locus ID	11430
UniProt ID	Q9R0H0
Gene Summary	This gene encodes a member of the acyl-coenzyme A oxidase family. The encoded protein is localized to peroxisomes and is the first enzyme of the fatty acid beta-oxidation pathway, which catalyzes the desaturation of acyl-coenzyme A to 2-trans-enoyl-coenzyme A. Disruption of this gene results in microvesicular steatohepatitis, spontaneous peroxisome proliferation, and the eventual development of hepatocellular carcinomas. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2012] Transcript Variant: This variant (2) has multiple differences in the coding region but maintains the reading frame, compared to variant 1. This variant encodes isoform 2, which is the same length as isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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