Palld (NM_001293774) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2410003B16Rik; 6030492A02
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228608 representing NM_001293774 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGCTCTGGCCTCCCGCGCTACCGCCATGCAGTCCTCCGGCTCCTTCAACTACGCGCGCCCTAAGC AGTTCATCGCGGCGCAGAACCTGGGTCCCGCATCGGGGCTGCCCACGCCAACCTCCAGCCCCAGCTCCTC CAGCTTGCCGTCACCATTGTCCCCCACGCCCAGGCCCTTCGGCCGCGCGCCCGGGCCGCCCTTCGTGGAA CCCGAGGCCATGTGGGGCCCGTCCTCGCCCTCGCCGCCACCGCCGCCACCTCCGGTCTTCAGCCCCTCTG CAGCCTACCCGGTGCCCGACGTGTTCCCACTGCCACCGCCGCCGCCACCGCTACCTAGCTCCACCTCACA CTGCGCCTCGCCCGCCCGCTTCGGCCCCAGCCAGACGCCCGCAGCCTTCCTCAGCGCCCTGCTGCCCTCT CAGCCACCGCCTGTCGCTGTCAACGCCCTGGGGCTGCCCAAGGGAGTCACCCCCGCGGGGTTCCCAAAGA AGTCCAGTAGAACTGCTAGAATTGCCTCTGATGAGGAGATTCAAGGCACAAAGGATGCTGTCATCCAAGA CCTGGAACGGAAGCTTCGCTTCAAGGAGGACCTTCTGAACAATGGCCAACCGAGGCTAACCTATGAGGAA AGAATGGCTCGCCGCCTGCTTGGAGCCGACAGCGCAAACGTCTTCAACATCCAGGAGCCAGAGGAAACGG CAGCCAATCAGGAGTACAAAGTCTCTAGCTGCGAGCAGAGGCTGATTAGCGAGATTGAGTACCGGCTGGA GCGCTCCCCTGTGGATGAGTCGGGAGACGAGGTGCAGGATCCAGATGTGCCTGTGGAGAACGCAACAGCT CCCTTCTTTGAGATGAAGCTGAAACACTACAAGATCTTTGAGGGGATGCCGGTGACTTTCACGTGTCGAG TGGCTGGGAATCCAAAGCCAAAGATCTATTGGTTTAAAGATGGGAAGCAGATTTCTCCGAAGAGCGATCA CTACACCATTCAGAGAGACCTTGATGGGACCTGCTCTCTCCACACCACGGCCTCTACCCTAGACGACGAT GGGAACTACACCATCATGGCTGCCAACCCTCAGGGTCGCGTCAGTTGTACAGGAAGGCTAATGGTACAGG CTGTCAACCAAAGAGGCCGCAGTCCCCGCTCTCCCTCAGGCCATCCTCATGCCAGAAGGCCTCGCTCTCG ATCACGGGACAGTGGAGATGAAAACGAGCCCATTCAGGAGCGATTCTTCAGACCTCACTTCCTGCAGGCT CCTGGAGACCTGACCGTTCAGGAAGGCAAGCTCTGCAGGATGGACTGCAAGGTCAGTGGGTTACCAACCC CAGATCTCAGCTGGCAACTAGATGGAAAGCCCATACGCCCCGACAGTGCTCACAAGATGCTGGTCCGTGA GAATGGGGTCCACTCCCTCATTATAGAGCCAGTCACGTCCCGGGACGCCGGCATCTACACATGTATTGCC ACCAACAGAGCAGGACAGAACTCGTTTAACCTGGAGCTTGTGGTTGCTGCTAAGGAAGCACACAAGGCCC CTGTGTTTATGGAGAAGCTACAGAACACGGGGGTTGCTGATGGATACCCAGTGCGGCTGGAATGCCGTGT CTCGGGAGTGCCGCCACCTCAGATATTTTGGAAGAAAGAAAATGAATCGCTCACTCACAGCACTGAGCGA GTAAGCATGCACCAGGATAATCATGGCTACATCTGCCTGCTCATCCAGGGAGCCACAAAGGAAGACGCTG GGTGGTACACTGTGTCCGCCAAGAACGAAGCAGGCATTGTGTCCTGCACTGCCCGGCTGGATGTCTACAC CCAGTGGCACCAGCAGCCACAGACCACCAAGCCAAAAAAAGTACGGCCCTCGGCCAGTCGCTACGCAGCA CTTTCGGACCAGGGACTAGACATCAAAGCCGCTTTCCAACCTGAAGCCAGCCCATCTCACCTGACTCTGA ACAGCGGCTTGGTAGAAAGTGAAGACCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001293774
Insert Size	1992 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001293774.1, NP_001280703.1
RefSeq Size	4224 bp
RefSeq ORF	1992 bp
Locus ID	72333
UniProt ID	Q9ET54
Gene Summary	Cytoskeletal protein required for organization of normal actin cytoskeleton. Roles in establishing cell morphology, motility, cell adhesion and cell-extracellular matrix interactions in a variety of cell types. May function as a scaffolding molecule with the potential to influence both actin polymerization and the assembly of existing actin filaments into higher-order arrays. Binds to proteins that bind to either monomeric or filamentous actin. Localizes at sites where active actin remodeling takes place, such as lamellipodia and membrane ruffles. Different isoforms may have functional differences. Involved in the control of morphological and cytoskeletal changes associated with dendritic cell maturation. Involved in targeting ACTN to specific May be required for the initiation of neural tube closure.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence and lacks an alternate in-frame exon compared to variant 1. The resulting isoform (3) is shorter at the N-terminus and lacks an alternate internal segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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