Jph2 (NM_001205076) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1110002E14Rik; JP-2; Jp2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228704 representing NM_001205076 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCGGGGGCCGCTTTGACTTTGATGATGGCGGGGCGTATTGTGGGGGCTGGGAAGGGGGAAAGGCAC ACGGGCACGGACTGTGCACCGGCCCCAAGGGCCAGGGTGAATACTCGGGCTCCTGGAATTTTGGCTTTGA AGTGGCAGGCGTCTATACCTGGCCCAGTGGGAATACCTTTGAGGGATATTGGAGCCAGGGCAAACGACAT GGGCTTGGCATAGAGACCAAGGGGCGCTGGCTCTACAAGGGGGAGTGGACGCATGGCTTTAAGGGGCGCT ACGGAATCCGGCAGAGCACAAACAGTGGTGCCAAGTACGAGGGCACTTGGAATAACGGCCTACAGGACGG CTATGGCACGGAGACCTACGCAGACGGAGGAACCTATCAAGGCCAATTCACCAACGGCATGCGCCATGGC TACGGTGTGCGCCAAAGCGTGCCCTACGGGATGGCAGTGGTGGTGCGTTCTCCGCTGCGCACTTCTCTGT CCTCGCTGCGCAGCGAGCACAGCAATGGAACGGTGGCTCCGGACTCACCGGCGGCAGATGGGCCCATGCT GCCTTCGCCCCCAGTGCCGCGCGGTGGTTTCGCGCTCACTCTGCTGGCCACAGCAGAGGCCGCGCGACCC CAAGGGCTGTTCACGCGTGGCACACTGCTGGGTCGGCTGCGACGCTCAGAATCACGCACATCACTGGGCA GCCAGCGGAGCCGCTTGAGCTTTCTCAAGAGCGAGCTGAGTTCCGGAGCCAGCGATGCCGCATCCACTGG CAGCCTGGCCGAGGGCGCTGAGGGCCCCGACGACGCGGCTGCGCCCTTCGATGCCGACATCGACGCCACC ACCACGGAAACCTACATGGGCGAGTGGAAGAACGACAAGCGCTCGGGCTTCGGCGTGAGCGAGCGTTCCA GCGGCCTGCGCTACGAGGGCGAGTGGCTGGACAACCTGCGCCACGGCTACGGCCGCACCACGCTGCCCGA CGGCCACCGCGAGGAGGGCAAGTACCGCCACAATGTGCTGGTCAAGGGCACCAAGCGCCGCGTGCTGCCG CTCAAGAGCAGCAAGGTCCGCCAGAAGGTGGAGCACGGGGTGGAGGGCGCCCAGCGCGCAGCAGCCATCG CGCGCCAGAAGGCCGAGATTGCCGCCTCCAGGACAAGCCATGCCAAAGCCAAGGCAGAGGCAGCAGAACA GGCTGCCCTGGCTGCCAACCAGGAGTCCAACATCGCCCGTACATTGGCCAAGGAGCTGGCTCCAGACTTC TACCAGCCAGGTCCGGAGTATCAGAAGCGTCGGCTGCTCCAGGAGATCCTGGAGAACTCTGAGAGCTTGC TGGAGCCCCCAGAGCGGGGTCTGGGCACCGGCCTCCCGGAGCGGCCCCGGGAAAGCCCGCAGCTGCATGA GCGCGAGACCCCGCAGCCCGAGGGCGGACCCCCGTCTCCGGCCGGGACGCCCCCGCAACCCAAGAGGCCC CGGCCCGGAGCGTCAAAGGACGGCCTGCTGAGTCCAGGCTCCTGGAACGGGGAGCCCGGCGGAGAGGGCA GCCGGCCCGCCACGCCGTCGGATGGCGCCGGTCGTCGCAGCCCCGCGCGCCCCGCCTCGGAGCACATGGC CATCGAGGCGCTGCAGCCGCCGCCCGCGCCCTCGCAGGAGCCCGAGGTAGCAATGTACCGCGGCTACCAT AGCTACGCCGTGCGCACCGGGCCACCCGAGCCTCCGCCCTTGGAGGATGAGCAGGAGCCCGAGCCGGAGC CCGAGCCCGAGGTCCGGCGATCCGACTCGGCGCCCCCGTCCCCCGTCTCCGCCACCGTCCCGGAGGAGGA GCCCCCTGCGCCGCGAAGCCCGGTGCCTGCCAAGCAAGCCACTCTGGAGCCCAAGCCCATCGTCCCCAAA GCAGAGCCCAAGGCCAAGGCGCGCAAGACAGAGGCCCGAGGACTGAGCAAGGCCGGTGCCAAGAAGAAGG GCCGTAAGGAAGTGGCGCAGGCGAAGGAGGCCGAGGTGGAGGTGGAGGAGGTACCCAACACCGTCCTCAT CTGTATGGTGATCTTGCTGAACATCGGCCTGGCTATCCTATTTGTTCACCTCCTGACTTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001205076
Insert Size	2091 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001205076.1, NP_001192005.1
RefSeq Size	4155 bp
RefSeq ORF	2091 bp
Locus ID	59091
UniProt ID	Q9ET78
Gene Summary	Junctophilin-2: Membrane-binding protein that provides a structural bridge between the plasma membrane and the sarcoplasmic reticulum and is required for normal excitation-contraction coupling in cardiomyocytes (PubMed:10949023, PubMed:19095005, PubMed:21339484). Provides a structural foundation for functional cross-talk between the cell surface and intracellular Ca(2+) release channels by maintaining the 12-15 nm gap between the sarcolemma and the sarcoplasmic reticulum membranes in the cardiac dyads (PubMed:10949023, PubMed:19095005, PubMed:21339484). Necessary for proper intracellular Ca(2+) signaling in cardiac myocytes via its involvement in ryanodine receptor-mediated calcium ion release (PubMed:10949023, PubMed:19095005, PubMed:21339484). Contributes to the construction of skeletal muscle triad junctions (PubMed:10949023).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

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