Acsl1 (NM_001302163) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ac; Acas; Acas1; Acs; Fac; Facl2; FACS; LACS 1; LACS1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228715 representing NM_001302163 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGTCCATGAATTGTTCCGGTATTTTCGAATGCCAGAGCTGATTGACATTCGGCAGTACGTGCGCA CCCTTCCAACCAACACCCTCATGGGGTTTGGGGCTTTTGCAGCGCTCACCACCTTCTGGTATGCCACCAG GCCTAAGGCCCTGAAGCCACCATGTGACCTCTCCATGCAGTCAGTGGAAATAGCGGGTACCACTGATGGT ATTCGAAGATCAGCAGTCCTTGAAGATGACAAGCTCTTGGTGTACTACTACGACGATGTCAGAACCATGT ACGATGGCTTCCAGAGGGGGATTCAGGTGTCAAATAATGGTCCTTGTTTAGGTTCTCGGAAGCCAAACCA GCCCTATGAGTGGATTTCCTACAAAGAGGTGGCAGAACTGGCTGAGTGCATAGGCTCCGGGCTGATCCAG AAGGGGTTCAAGCCTTGCTCCGAGCAGTTCATCGGCCTCTTCTCTCAAAACAGACCCGAGTGGGTGATCG TCGAGCAAGGATGCTTCTCTTACTCAATGGTGGTCGTCCCGCTCTATGACACCCTTGGAGCTGACGCCAT CACCTACATAGTGAACAAAGCTGAACTCTCTGTGATTTTTGCTGACAAGCCAGAAAAAGCCAAACTCTTA TTAGAAGGTGTAGAAAACAAGTTAACACCATGCCTTAAAATCATAGTCATCATGGACTCCTACGGCAGTG ATCTGGTGGAACGAGGCAAGAAGTGTGGGGTGGAAATCATCAGCCTCAAAGCTCTGGAGGACCTTGGAAG AGTGAACAGAGTGAAGCCCAAGCCTCCAGAACCCGAAGATCTTGCGATAATTTGTTTCACAAGTGGAACT ACAGGCAACCCCAAAGGAGCAATGATCACTCACCAAAACATTATAAACGACTGCTCAGGTTTTATAAAAG CAACAGAGAGTGCATTCATCGCTTCCACAGATGATGTGCTGATATCTTTCTTGCCTCTCGCCCATATGTT TGAGACCGTTGTAGAGTGTGTAATGCTGTGTCATGGAGCTAAGATAGGATTTTTCCAAGGAGATATCAGG CTGCTTATGGACGACCTCAAGGTGCTTCAGCCCACCATCTTCCCTGTGGTTCCCAGGCTGCTGAACCGGA TGTTCGACAGAATTTTTGGACAAGCAAACACTTCCTTGAAGCGATGGCTGTTGGACTTTGCCTCCAAAAG GAAAGAGGCGGAGCTTCGCAGTGGCATCGTCAGAAACAACAGCCTGTGGGATAAACTCATCTTCCACAAG ATACAGTCGAGCCTGGGTGGGAAAGTCCGGCTGATGATCACAGGAGCAGCCCCGGTGTCTGCCACAGTGC TGACGTTTCTGAGGACAGCGCTCGGCTGCCAGTTCTATGAAGGCTACGGACAGACCGAGTGCACTGCTGG TTGCTGCCTGAGCTTGCCCGGAGACTGGACGGCAGGCCATGTTGGAGCCCCCATGCCTTGCAATTATGTA AAGCTTGTGGATGTGGAAGAAATGAATTACCTGGCATCCAAGGGCGAGGGTGAGGTGTGTGTGAAAGGGG CAAATGTGTTCAAAGGCTACTTGAAAGACCCAGCAAGAACAGCTGAAGCCCTGGATAAAGATGGCTGGTT ACACACGGGGGACATTGGAAAATGGCTGCCAAATGGCACCTTGAAGATTATCGACAGGAAAAAGCACATA TTTAAACTAGCCCAAGGAGAGTACATAGCACCAGAAAAGATTGAAAATATCTACCTGCGGAGTGAAGCCG TGGCCCAGGTGTTTGTCCACGGAGAAAGCTTGCAGGCCTTTCTCATAGCAGTTGTGGTACCCGACGTTGA GAGCCTACCGTCCTGGGCACAGAAGAGAGGCTTACAAGGGTCCTTCGAAGAACTGTGCAGGAACAAGGAT ATCAATAAAGCTATCCTGGACGACTTGTTGAAACTTGGGAAGGAAGCCGGTCTGAAGCCATTTGAACAGG TCAAAGGCATTGCTGTGCACCCGGAATTATTTTCTATTGACAACGGCCTTCTGACTCCAACACTGAAGGC GAAGAGGCCAGAGCTACGGAACTATTTCAGGTCGCAGATAGATGAACTGTACGCCACCATCAAGATCTAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001302163
Insert Size	2100 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001302163.1, NP_001289092.1
RefSeq Size	3927 bp
RefSeq ORF	2100 bp
Locus ID	14081
UniProt ID	P41216
Gene Summary	The protein encoded by this gene belongs to a family of acyl coenzyme A synthetase proteins, which convert long chain fatty acids to acyl CoA products via an ATP-dependent pathway. This enzyme is enriched in heart, liver and adipose tissue, where it functions in lipid synthesis and mitochondrial and peroxisomal beta-oxidation. In addition, it is expressed in monocytes and macrophages where it appears to have a functionally distinct role in mediating inflammatory and innate immune responses. A pseudogene of this gene is found on chromosome 5. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] Transcript Variant: This variant (1) represents the longer transcript.

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