Sh2d3c (NM_001252547) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Chat; Nsp3; Shep1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228721 representing NM_001252547 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGCAGTGGGCCGAAGGTGCTCTGCGCTAGAGCCCCGAAGGACTGCTGCAGAGCTGGAGGCCGCTG GGGACTATGTGAAGTTCTCCAAGGAGAAGTACATTCTTGACTCCTCGCCAGAGAAACTGCACAAGGAGCT GGAAGAGGAACTGAAGCTCAGCAGCACGGACCTCCGTAGCCATGCCTGGTACCACGGACGCATCCCTCGT GAGGTCTCTGAGACTCTGGTGCAGCGGAACGGCGACTTCCTCATCCGAGACTCACTCACCAGCCTAGGGG ACTATGTGCTCACGTGCCGCTGGCACAATCAGGCCTTGCACTTCAAGATCAACAAGGTGGTGGTGAAGGC CGGAGAGAGCTACACCCACATCCGGTACCTCTTCGAGCAGGAGAGCTTCGACCACGTGCCTGCCCTCGTG CGCTACCACGTGGGCAGCCGTAAGGCCGTGTCCGAACAGAGCGGGGCTATCATCTACTGCCCTGTCAACC GCACCTTCCCACTGCGCTACCTCGAGGCCAGCTATGGCCTGAGCCAGGGCAGCAGCAAGACTGCCAGCCC AGCCAGCCCCTCAGGCTCCAAGGGCAGCCACATGAAGAGGCGAAGCATTACCATGACTGATGGACTCACC ACTGACAAGGTCACCCGCAGCGATGGCTGCCCCAACAGCACCTCATTACCCCATCCTCGAGACTCCATCC GAAACTGTGCCCTCAGCATGGACCAGATCCCAGATCTCCACTCACCCCTGTCTCCTATCTCCGAGAGCCC CAGCTCTCCTGCCTATAGCACTGTAACCCGAGTCCATGCTCCTTCTGCCACCCCCTCTACTTCAGCACAG CCTGCCTCCCCAGTTGCCCGCCGCTCCAGCGAACCTCAGCTATGTCCTGGAAATACTCCAAAGCCTCCTG GGGAGTCAGACAGGGCCCCTCACGCAAGCCCATCCCACACCCTCTGCAAGGCCTCGCCATCACCATCACT CAGCAGCTACAGTGACCCGGACTCTGGTCACTACTGTCAGCTCCAGCCTCCGGTCCGTGGCAGCCGAGAG CAGGCCGCAGGAGAGACTCCCCGGAAGGCTAGGGGCTCCGGAGAGAGGCAAAAGGAACTGTTAGAAAATG GAGTCTCTGACGGGGAGTGGGGCAAGACCTTCACAGTCCCTGTAGTAGAAGCCACCTCCTCTTTCAACCT GGCTACCTTCCAGTCCCAGCTGATCCCCAAGGAGAACCGGCCTCTGGAGGTGGCCCTTCTGCGCAAGGTC AAGGAGCTGCTGTCTGAGGTGGATGCCCGGACACTGGCCCGGCATGTCACCAAGGTTGACTGCCTGGTTG CTAGGATACTGGGCGTTACCAAGGAGATGCAGACCCTAATGGGAGTCCGCTGGGGCATGGAGCTGCTCAC CCTCCCCCATGGCCGGCAGCTACGACTAGACCTGCTGGAAAGGTTCCACACCATGTCCATCATGCTGGCA GTGGATATCCTGGGCTGCACAGGCTCGGCGGAGGAGCGGGCAGCTCTGCTGCACAAGACCATACAGCTGG CGGCCGAGCTGCGGGGGACGATGGGCAACATGTTCAGCTTCGCCGCTGTCATGGGCGCCCTGGAGATGGC CCAGATTTCAAGGCTGGAGCAGACATGGATGACCCTGCGACAGCGACACACGGAGGGTGCCATTCTGTAC GAGAAGAAGCTCAAGCCTTTCCTCAAGAGCCTGAACGAGGGCAAAGAAGGCCCACCGCTGAGCAACACCA CGTTTCCACACGTACTTCCTTTCATTACTCTGCTGGAATGTGACTCTGCCCCCGCCGAGGGTCCTGAACC TTGGGGCAGCACAGAACACGGTGTGGAGGTTGTGCTAGCCCACCTGGAGGCCGCCCGCACTGTGGCCCAC CACGGGGGCCTCTACCACACCAACGCTGAGGTCAAGCTGCAAGGGTTCCAAGCCCGACCGGAGCTCCTGG AAGTATTCAGCACAGAGTTCCAGATGCGGCTCCTCTGGGGCAGCCAGGGTGCCAACAGCAGCCAGGCCTG GCGCTATGAGAAGTTTGACAAAGTCCTCACTGCCTTGTCCCACAAGCTGGAACCTGCCATCCGCTCTAGC GAGCTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252547
Insert Size	2109 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252547.1, NP_001239476.1
RefSeq Size	2619 bp
RefSeq ORF	2109 bp
Locus ID	27387
UniProt ID	Q9QZS8
Gene Summary	Eph receptor-binding protein which may be a positive regulator of TCR signaling. Binding to BCAR1 is required to induce membrane ruffling and promote EGF-dependent cell migration.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is shorter and has a distinct N-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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