Xpnpep2 (NM_001289729) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	9030008G12Rik; mAPP
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228825 representing NM_001289729 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAAGCCTACTGGCAGTGCTACCCCTGGCTTGTTCTCCTCTGTGCATGTGCCTGGAGCTACCCAG AGCCCAAGTACCTTGGAAGGGAAGATGTGAGAAACTGTTCAACCAGCCCTGAGCGCCTGCCAGTTACTGC CGTCAATACAACAATGCGGCTTGCAGCCCTCCGCCAACAGATGGAGACCTGGAATCTCTCTGCCTACATC ATCCCAGACACGGATGCACACATGAGTGAGTACATTGGCAAACCTGATAAGAGGCGAGAGTGGATTTCAG GCTTCACAGGGTCTGCAGGTACTGCTGTGGTGACCATGGGGAAAGCAGCTGTCTGGACCGATAGTCGTTA CTGGACTCAGGCCGAAAGGCAGATGGACTGCAACTGGGAGCTGCACAAGGAAGTTAGCATTTCTTCCATT GTCGCCTGGATCTTAGCTGAAGTCCCTGATGGACAGAACGTGGGCTTCGACCCCTTCCTCTTTTCTGTTG ATTCCTGGAAAAATTATGATCAAGGATTCCAAGACTCCAGCAGACACCTGTTATCGGTTACAACCAACCT TGTTGACGTGGCATGGGGGTCAGAGAGGCCCCCAGTGCCAAGCCAACCCATTTATGCCCTGCCGAAAGAA TTTACAGGGAGCACTTGGCAGGAAAAAGTATCCGCTGTCCGAAGCTATATGGAGCACCATGCCAAGACTC CAACTGGGGTCCTTCTATCTGCACTTGATGAGACAGCCTGTGAGTGGGGATTTTTGAACATGGGTCAGTC TGGTAGTCCCAAGCTTCCTGGACCTTGCAACATACAGCTTTTCCTAAAACAACAACATATTCACCAGCTC CTACGAGCTCCCAAGATCATAGTCTGTGGAAACTCAGAGGGGAAGAGAGCAGCTGTTTGGGGCTATAGAA CAAGGAAGCCAGGGAGCTCCAAGCAAGTGGGGCTCTTCAACCTTCGTAGCAGTGACATCCCCTATAACCC CTTCTTCTACTCCTATGCACTGCTCACGAACTCCTCCATCAGGTTGTTTGTCAACAAGAGTCGCTTTAGC CTTGAGACCTTGCAGTACCTGAATACAAACTGCACATTACCCATGTGTGTGCAACTTGAGGACTACAGTC AAGTTCGTGACAGTGTGAAGGCCTATGCCTCAGGCGATGTGAAAATCTTAATTGGGGTCAGCTATACCAC CTATGGGGTCTATGAAGTGATACCCAAGGAGAAACTCGTGACAGACACCTACTCCCCAGTGATGTTAATC AAGGCTGTGAAGAACAGCAAGGAGCAGGCCCTTTTGAAGAGCAGCCACGTGCGGGACGCTGTGGCTGTGA TCCAGTACTTGGTCTGGTTGGAGAAGAACGTGCCCAAAGGCACGGTGGATGAGTTTTCTGGGGCAGAATA CATTGATGAGTTACGACGGAATGAAAACTTCTCCTCTGGACCCAGTTTTGAAACCATCTCTGCTAGTGGC CTGAATGCCGCCCTGGCCCATTACAGCCCAACAAAAGAGCTGCACCGTAAGCTGTCCTCAGATGAGATGT ACCTGGTGGATTCTGGGGGGCAATACTGGGATGGGACCACAGATATCACCAGAACAGTACATTGGGGCAC TCCTACTGCCTTCCAAAAGGAGGCCTATACTCGAGTGCTAATGGGAAACATCGATCTGTCCAGACTTGTC TTTCCTGCTGCTACATCAGGGAGAGTGATAGAAGCCTTTGCCCGAAGAGCCTTATGGGAAGTTGGGCTCA ATTATGGTCATGGGACAGGCCATGGCATTGGCAACTTCCTCTGTGTGCATGAGTGGCCAGTGGGATTCCA GTATAACAACATTGCCATGGCCAAGGGCATGTTCACTTCCATTGAACCTGGATACTACCATGATGGGGAG TTTGGAATCCGTCTTGAAGATGTCGCCCTTGTGGTAGAAGCAAAAACCAAGTACCCAGGGGACTACCTGA CTTTTGAATTGGTGTCCTTTGTGCCCTATGACCGAAACCTCATCGATGTCAGGCTGCTGTCCCCAGAGCA GCTCCAGTACCTGAACCGCTACTATCAGACCATTCGTGAGAATGTAGGCCCAGAGCTGCAGCGTCGCCAG CTGCTGGAGGAGTTTGCATGGTTGGAGCAGCACACGGAGCCCCTGTCAGCCAGGGCCCCTCATATCATCT CCTGGACCTCTCTGTGGGTGGCCTCTGCCCTTGCCATCCTCAGCTGGAGTAGCTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289729
Insert Size	2226 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001289729.1, NP_001276658.1
RefSeq Size	3699 bp
RefSeq ORF	2226 bp
Locus ID	170745
UniProt ID	B1AVD1
Gene Summary	Membrane-bound metalloprotease which catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro. May play a role in the metabolism of the vasodilator bradykinin.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) represents the longer transcript and encodes the longer isoform (2).

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