Mme (NM_001289462) Mouse Untagged Clone

CAT#: MC228849

Mme (untagged) - Mouse membrane metallo endopeptidase (Mme), transcript variant 1



  "NM_001289462" in other vectors (1)

CNY 7,220.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (3)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Untagged Clone
Tag Tag Free
Synonyms 6030454K05Rik; C85356; CALLA; CD10; NEP; SFE
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MC228849 representing NM_001289462
Red=Cloning site Blue=ORF Orange=Stop codon

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGAAGATCAGAAAGTCAGATGGATATAACTGATATCAATGCTCCAAAGCCAAAGAAGAAACAGCGAT
GGACTCCCCTGGAGATCAGCCTCTCTGTGCTTGTCTTGCTCCTGACTATCATAGCGGTGACAATGATCGC
TCTCTATGCAACCTATGATGATGGCATTTGCAAATCATCAGACTGCATAAAATCAGCTGCTCGACTGATT
CAGAATATGGATGCCTCTGTTGAGCCATGTACAGACTTCTTCAAGTATGCTTGTGGAGGCTGGTTGAAAC
GCAACGTCATTCCCGAGACCAGTTCCCGATACAGTAATTTTGACATTTTAAGAGATGAGCTAGAAGTCAT
TTTGAAAGATGTCCTTCAAGAACCCAAAACTGAAGACATAGTAGCAGTGCAGAAAGCAAAAACCTTGTAC
AGATCGTGTATAAATGAGTCTGCTATTGATAGCAGAGGCGGACAACCTCTACTCAAACTGTTACCAGATA
TATATGGGTGGCCAGTAGCATCAGATAACTGGGATCAAACATATGGCACTTCTTGGACAGCTGAGAAATC
CATTGCACAACTGAATTCTAAATATGGGAAAAAGGTCCTCATTAATTTTTTTGTTGGCACTGATGATAAG
AATTCTACCCAGCATATAATTCATTTTGACCAGCCTCGACTTGGCCTCCCTTCCAGAGACTACTATGAGT
GCACTGGAATATATAAAGAGGCTTGCACAGCATATGTGGATTTTATGATTTCTGTGGCCAGACTGATTCG
TCAGGAACAAAGTTTGCCTATTGATGAAAACCAGCTCTCTTTGGAAATGAATAAAGTTATGGAATTGGAA
AAAGAAATTGCCAATGCTACAACTAAACCAGAAGACCGAAATGACCCAATGCTGCTTTATAACAAAATGA
CATTGGCCAAGCTGCAGAACAACTTTTCCCTGGAGGTCAATGGGAAGTCATTCAGCTGGTCAAATTTCAC
AAATGAAATCATGTCAACTGTGAATATTAATATTCAAAATGAGGAAGAAGTTGTTGTCTATGCTCCAGAA
TATTTAACCAAACTTAAGCCTATTCTTACCAAATATTCTCCCAGAGATCTTCAAAATTTAATGTCCTGGA
GGTTCATAATGGATCTTGTAAGCAGCCTCAGCCGAAACTACAAGGAGTCCAGAAATGCTTTCCGCAAGGC
CCTTTATGGGACTACATCAGAAACTGCAACATGGAGACGGTGTGCTAACTACGTCAATGGGAATATGGAG
AATGCTGTGGGGAGGCTTTATGTGGAAGCGGCTTTTGCTGGAGAGAGCAAGCACGTGGTTGAAGACTTGA
TTGCACAAATCCGGGAAGTTTTTATTCAGACTTTGGATGACCTTACTTGGATGGATGCTGAGACAAAAAA
GAAAGCTGAAGAAAAGGCCTTGGCAATTAAAGAAAGGATTGGCTATCCTGATGACATCATTTCCAATGAG
AACAAACTGAATAATGAGTATCTTGAGTTGAACTACAGGGAAGATGAATACTTTGAGAACATAATTCAAA
ATTTGAAATTCAGCCAAAGCAAGCAGCTAAAGAAGCTCCGAGAAAAAGTGGACAAAGATGAGTGGATAAG
TGGAGCAGCTGTAGTCAATGCATTTTATTCCTCAGGCCGAAATCAGATAGTCTTCCCCGCTGGCATTTTG
CAGCCCCCATTCTTCAGTGCTCAGCAATCCAACTCATTGAACTATGGGGGCATCGGCATGGTCATTGGAC
ATGAAATCACACATGGATTTGATGACAATGGAAGAAATTTCAATAAAGATGGAGACCTCGTTGACTGGTG
GACGCAGCAGTCGGCAAATAATTTTAAAGACCAATCCCAGTGTATGGTATACCAGTATGGAAACTTTTCC
TGGGACCTAGCAGGTGGACAGCATCTCAATGGAATTAATACACTAGGAGAAAACATTGCTGATAATGGAG
GTATTGGCCAAGCATACAGAGCCTATCAGAATTATGTTAAAAAGAATGGTGAAGAAAAATTACTCCCTGG
ACTTGACCTCAATCACAAACAACTATTTTTCCTGAACTTTGCCCAGGTGTGGTGTGGGACCTACCGGCCA
GAGTATGCAGTCAATTCTATTAAAACAGATGTACACAGTCCTGGAAATTTCAGGATCATTGGGACTTTGC
AGAACTCTGCCGAGTTTGCAGATGCCTTTCATTGCCGCAAGAATTCATACATGAATCCAGAAAGGAAATG
TCGGGTTTGGTGA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites SgfI-MluI     
ACCN NM_001289462
Insert Size 2253 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001289462.1, NP_001276391.1
RefSeq Size 6088 bp
RefSeq ORF 2253 bp
Locus ID 17380
UniProt ID Q61391
Gene Summary Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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