Tle1 (NM_001285529) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	C230057C06Rik; Estm14; Grg-1; Grg1; Tle4l
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC228900 representing NM_001285529 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGCAGAGCCGGCACCCAACGCCGCACCAAGCTGCAGGCCAGCCCTTTAAGTTCACTATCCCGG AGTCTCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGTCTTAAATTGGAGTG TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTACGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAACACCATTTGTGCCCAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTGGAACGTGCCAAACAGGTGACCATGGC AGAGTTGAATGCCATCATCGGGCAGCAGTTGCAAGCTCAGCATCTCTCCCATGGCCATGGACCCCCAGTA CCTCTCACGCCTCACCCTTCAGGACTTCAGCCTCCTGGCATCCCGCCCCTTGGGGGCAGTGCCAGCCTTC TCGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCATGATGC AGAGCGCCACAGAGACAGAGAGCCTGGCACGAGTAATTCCCTCTTGGTCCCAGACAGCCTAAGAGGCACA GATAAGCGCAGAAATGGTCCAGAGTTTTCCAGTGACATCAAAAAAAGGAAGGTGGATGATAAGGATAACT ATGACAGTGATGGGGACAAGAGTGATGACAACTTAGTTGTGGATGTGTCTAACGAGGACCCTTCTTCTCC ACATGCAAGCCCCACACACTCACCCCGGGAAAACGGAATTGACAAAAACCGTCTGCTGAAGAAAGATGCT TCAGGTAGCCCGGCATCCACAGCCTCCTCTGGAAGTTCCTCTTCCCTGAAATCCAAAGAAGTGAGCCTGC ATGAAAAAGCCAACACTCCTGTTCTGAAATCCAGCACACCGACGCCTCGGAGCGACATGCCAACCCCAGG CACCAGCGCTACTCCAGGCCTCCGTCCAGGTCTTGGCAAGCCTCCAGCCATGGAACCCCTTGTCAACCAA GCAGCAGCTGGCCTGAGGACGCCCCTGGCGGTGCCTGGCCCATACCCTGCCCCCTTTGGCATGGTGCCCC ATGCGGGCATGAACGGAGAGCTGACCAGCCCTGGTGCTGCCTATGCAGGTCTACACAGCATGTCTCCACA GATGAGCGCTGCAGCTGCTGCAGCTGCTGCTGCTGTGGTGGCCTATGGGCGCTCCCCAATGGTTGGTTTT GATCCTCCTCCTCACATGAGAGTACCTTCTATCCCCCCCAACCTGGCAGGAATACCTGGAGGGAAACCAG CATACTCCTTCCACGTTACTGCTGATGGCCAAATGCAGCCTGTTCCTTTTCCCCCTGATGCCCTCATTGG ACCTGGGATTCCCCGACATGCTCGGCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGTGCAGTGACC ATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGCGTTAAGGTGTGGGACATCAGCCACC CCGGCAACAAGAGCCCCGTCTCTCAGCTGGATTGTCTGAATAGAGATAACTACATCCGTTCCTGTAAATT GCTACCTGATGGCTGTACTCTCATAGTGGGAGGGGAAGCCAGTACTCTATCCATCTGGGACTTGGCAGCT CCAACTCCCCGCATAAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGTTATGCTCTGGCCATCAGCCCCG ACTCCAAGGTCTGCTTCTCATGCTGCAGTGACGGCAACATCGCAGTGTGGGACCTGCACAACCAGACCCT GGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTC TGGACAGGCGGTTTAGACAACACTGTGAGGTCCTGGGACCTGAGAGAAGGGAGGCAGCTGCAGCAGCATG ACTTCACTTCACAGATCTTCTCTTTGGGATATTGCCCAACTGGGGAGTGGCTTGCTGTGGGCATGGAAAG CAGCAACGTGGAAGTTCTGCATGTGAACAAGCCTGACAAGTACCAGCTGCACCTCCACGAGAGCTGCGTG CTGTCCCTAAAGTTTGCTTATTGTGGCAAATGGTTTGTGAGTACTGGAAAAGACAACCTCCTAAATGCCT GGCGGACACCATATGGAGCCAGCATCTTCCAGTCCAAAGAATCTTCATCAGTGCTTAGCTGTGACATCTC TGTGGATGATAAATACATAGTCACTGGCTCAGGGGACAAAAAGGCTACCGTTTATGAGGTCATCTACTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001285529
Insert Size	2310 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001285529.1, NP_001272458.1
RefSeq Size	11547 bp
RefSeq ORF	2310 bp
Locus ID	21885
Gene Summary	Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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