Tle1 (NM_001285529) Mouse Tagged ORF Clone
CAT#: MR231111
- TrueORF®
Tle1 (myc-DDK-tagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1), transcript variant 2
"NM_001285529" in other vectors (1)
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CNY 7,700.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | C230057C06Rik; Estm14; Grg-1; Grg1; Tle4l |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR231111 representing NM_001285529
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCCGCAGAGCCGGCACCCAACGCCGCACCAAGCTGCAGGCCAGCCCTTTAAGTTCACTATCCCGG AGTCTCTGGACCGGATTAAAGAGGAATTCCAGTTCCTGCAGGCGCAGTATCACAGTCTTAAATTGGAGTG TGAGAAACTGGCAAGTGAAAAGACAGAAATGCAGAGGCACTACGTGATGTATTATGAAATGTCATATGGA TTAAACATTGAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAACACCATTTGTGCCCAAGTCATCC CATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTGGAACGTGCCAAACAGGTGACCATGGC AGAGTTGAATGCCATCATCGGGCAGCAGTTGCAAGCTCAGCATCTCTCCCATGGCCATGGACCCCCAGTA CCTCTCACGCCTCACCCTTCAGGACTTCAGCCTCCTGGCATCCCGCCCCTTGGGGGCAGTGCCAGCCTTC TCGCGCTGTCTAGTGCTCTGAGTGGGCAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCATGATGC AGAGCGCCACAGAGACAGAGAGCCTGGCACGAGTAATTCCCTCTTGGTCCCAGACAGCCTAAGAGGCACA GATAAGCGCAGAAATGGTCCAGAGTTTTCCAGTGACATCAAAAAAAGGAAGGTGGATGATAAGGATAACT ATGACAGTGATGGGGACAAGAGTGATGACAACTTAGTTGTGGATGTGTCTAACGAGGACCCTTCTTCTCC ACATGCAAGCCCCACACACTCACCCCGGGAAAACGGAATTGACAAAAACCGTCTGCTGAAGAAAGATGCT TCAGGTAGCCCGGCATCCACAGCCTCCTCTGGAAGTTCCTCTTCCCTGAAATCCAAAGAAGTGAGCCTGC ATGAAAAAGCCAACACTCCTGTTCTGAAATCCAGCACACCGACGCCTCGGAGCGACATGCCAACCCCAGG CACCAGCGCTACTCCAGGCCTCCGTCCAGGTCTTGGCAAGCCTCCAGCCATGGAACCCCTTGTCAACCAA GCAGCAGCTGGCCTGAGGACGCCCCTGGCGGTGCCTGGCCCATACCCTGCCCCCTTTGGCATGGTGCCCC ATGCGGGCATGAACGGAGAGCTGACCAGCCCTGGTGCTGCCTATGCAGGTCTACACAGCATGTCTCCACA GATGAGCGCTGCAGCTGCTGCAGCTGCTGCTGCTGTGGTGGCCTATGGGCGCTCCCCAATGGTTGGTTTT GATCCTCCTCCTCACATGAGAGTACCTTCTATCCCCCCCAACCTGGCAGGAATACCTGGAGGGAAACCAG CATACTCCTTCCACGTTACTGCTGATGGCCAAATGCAGCCTGTTCCTTTTCCCCCTGATGCCCTCATTGG ACCTGGGATTCCCCGACATGCTCGGCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGTGCAGTGACC ATCAGCAACCCCACAAGGCACGTGTACACAGGTGGCAAGGGCTGCGTTAAGGTGTGGGACATCAGCCACC CCGGCAACAAGAGCCCCGTCTCTCAGCTGGATTGTCTGAATAGAGATAACTACATCCGTTCCTGTAAATT GCTACCTGATGGCTGTACTCTCATAGTGGGAGGGGAAGCCAGTACTCTATCCATCTGGGACTTGGCAGCT CCAACTCCCCGCATAAAGGCAGAGCTGACATCCTCAGCCCCTGCCTGTTATGCTCTGGCCATCAGCCCCG ACTCCAAGGTCTGCTTCTCATGCTGCAGTGACGGCAACATCGCAGTGTGGGACCTGCACAACCAGACCCT GGTGAGGCAATTCCAGGGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTC TGGACAGGCGGTTTAGACAACACTGTGAGGTCCTGGGACCTGAGAGAAGGGAGGCAGCTGCAGCAGCATG ACTTCACTTCACAGATCTTCTCTTTGGGATATTGCCCAACTGGGGAGTGGCTTGCTGTGGGCATGGAAAG CAGCAACGTGGAAGTTCTGCATGTGAACAAGCCTGACAAGTACCAGCTGCACCTCCACGAGAGCTGCGTG CTGTCCCTAAAGTTTGCTTATTGTGGCAAATGGTTTGTGAGTACTGGAAAAGACAACCTCCTAAATGCCT GGCGGACACCATATGGAGCCAGCATCTTCCAGTCCAAAGAATCTTCATCAGTGCTTAGCTGTGACATCTC TGTGGATGATAAATACATAGTCACTGGCTCAGGGGACAAAAAGGCTACCGTTTATGAGGTCATCTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR231111 representing NM_001285529
Red=Cloning site Green=Tags(s) MFPQSRHPTPHQAAGQPFKFTIPESLDRIKEEFQFLQAQYHSLKLECEKLASEKTEMQRHYVMYYEMSYG LNIEMHKQTEIAKRLNTICAQVIPFLSQEHQQQVAQAVERAKQVTMAELNAIIGQQLQAQHLSHGHGPPV PLTPHPSGLQPPGIPPLGGSASLLALSSALSGQSHLAIKDDKKHHDAERHRDREPGTSNSLLVPDSLRGT DKRRNGPEFSSDIKKRKVDDKDNYDSDGDKSDDNLVVDVSNEDPSSPHASPTHSPRENGIDKNRLLKKDA SGSPASTASSGSSSSLKSKEVSLHEKANTPVLKSSTPTPRSDMPTPGTSATPGLRPGLGKPPAMEPLVNQ AAAGLRTPLAVPGPYPAPFGMVPHAGMNGELTSPGAAYAGLHSMSPQMSAAAAAAAAAVVAYGRSPMVGF DPPPHMRVPSIPPNLAGIPGGKPAYSFHVTADGQMQPVPFPPDALIGPGIPRHARQINTLNHGEVVCAVT ISNPTRHVYTGGKGCVKVWDISHPGNKSPVSQLDCLNRDNYIRSCKLLPDGCTLIVGGEASTLSIWDLAA PTPRIKAELTSSAPACYALAISPDSKVCFSCCSDGNIAVWDLHNQTLVRQFQGHTDGASCIDISNDGTKL WTGGLDNTVRSWDLREGRQLQQHDFTSQIFSLGYCPTGEWLAVGMESSNVEVLHVNKPDKYQLHLHESCV LSLKFAYCGKWFVSTGKDNLLNAWRTPYGASIFQSKESSSVLSCDISVDDKYIVTGSGDKKATVYEVIY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001285529 |
ORF Size | 2307 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001285529.1, NP_001272458.1 |
RefSeq Size | 11547 bp |
RefSeq ORF | 2310 bp |
Locus ID | 21885 |
MW | 83.4 kDa |
Gene Summary | Transcriptional corepressor that binds to a number of transcription factors. Inhibits NF-kappa-B-regulated gene expression. Inhibits the transcriptional activation mediated by FOXA2, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Unusual function as coactivator for ESRRG (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC228900 | Tle1 (untagged) - Mouse transducin-like enhancer of split 1, homolog of Drosophila E(spl) (Tle1), transcript variant 2 |
CNY 7,320.00 |