Mib2 (NM_001256107) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2210008I11Rik; skd; Zzank1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229214 representing NM_001256107 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGGACCCCCATGCAGGTGTGCAGGTGGGCATGCGTGTGGTACGCGGAATGGACTGGAAATGGG GCCAGCAGGATGGAGGTGAGGGCGGTGTGGGCACCGTGGTGGAGCTTGGCCGCCATGGCAGCCCCTCGAC CCCCGACCGTACAGTTGTCGTTCAGTGGGACCAGGGCACACGTACCAACTATCGAGCTGGCTACCAAGGT GCCCATGACCTGTTGCTCTATGACAACGCCCAAATCGGTATCCGCCACCCCAATATCATCTGTGACTGCT GCAAGAAACATGGGCTTCGGGGCATGCGTTGGAAGTGCCGCGTCTGCTTTGACTATGACCTCTGCACACA GTGCTACATGCACAACAAGCATGACCTGACCCATGCCTTCGAGCGCTATGAGACATCTCACTCACGCCCG GTTACGCTGAGCCCCCGACAGGGCCTCCCTCGGATCCCACTGAGGGGCATCTTTCAAGGAGCTAAGGTGG TACGAGGCCCTGACTGGGAATGGGGCTCACAAGATGGAGGGGAAGGCAAGACAGGTCGTGTGGTGGACAT CCGTGGCTGGGATGTGGAGACAGGCCGAAGTGTGGCCAGTGTGACGTGGGCAGATGGAACCACGAACGTG TACCGAGTGGGCCACAAGGGCAAGGTGGATCTCAGATGTGTTGGTGAGGCAGCTGGCGGCTTTTACTACA AGGAGCACCTCCCAAAGCTTGGCAAGCCAGCAGAGCTGCAGCGCAGGGTGAGTGCTGATGGCCAGCCCTT CCAGCGTGGGGACAAGGTCAAGTGTCTGCTGGACACAGATGTCCTAAGGGATATGCAAGAAGGCCATGGT GGCTGGAACCCTAGGATGGCAGAGATGGGCACCGTGCACCGCATCACAGACCGTGGGGACGTGCGTGTGC AGTTCAACCACGAGACCCGCTGGACCTTCCACCCTGGGGCTCTCACCAAGCACAACAGCTTCTGGGTGGG TGATGTGGTCCGGGTCATCGGTGACCTTGACACTGTGAAGCGACTTCAGGCCGGACATGGTGAATGGACC GACGACATGGCTCCCGCCCTGGGCCGAGTGGGGAAAGTGGTGAAGGTGTTTGGAGATGGAAACTTGCGTG TGGCGGTTGGCGGTCAGCGGTGGACCTTCAGCCCCTCTTGCCTAGTGGCCTACCGGCCTGAGGAAGACGC CAACCTGGATGTGGCTGAGCGTGCCAGAGAGAACAAAAGCTCACTGAGTGTGGCCCTGGACAAGCTTCGG ACCCAGAAGAGTGACCCAGAGCACCCGGGGAGGCTGGTAGTAGAGGCCGCACTAGGAAATGTAGCCCGGG CTCTAGATCTTCTGCGGAGGCATCCAGAGCAGGTGGACACCAAGAACCAGGGTAGAACTGCCCTGCAGGT GGCTGCTTACCTGGGCCAGGTAGAGCTGGTGCGGCTGCTGCTGCAGGCAAGAGCAAGTATGGACCTGCCG GATGATGAGGGCAACACTGTGCTGCACTACACAGCCATGGGGAACCAGCCTGAGGCCACAAGGGTGCTCC TGAGTGCAGGGTGTGCAGTGGATGCTCGGAATGGCACACGCAGCACAGCCCTACATGTGGCTGTACAGAG GGGCTTCCTAGAGGTGGTAAAGATCCTGTGTGAACGTGGTTGTGATGTCAACTTGCCGGATGCGCATGCA GACACACCCCTGCATTCTGCCATCTCTGCTGGTGCTGGTGCCAGCAGCATTGTTGAAGTCCTCACCGAGG TCCCTGGCATCGATGTCACTGCTACCAATAGCCAGGGCTTCACACTGCTACACCATGCATCCCTCAAGGG CCATGTGCTAGCAGTCAGGAAGATTCTGGCAAGGGCCCGGCAGCTGGTGGATGCCAAGAAGGAGGATGGC TTTACCGCACTGCATCTGGCAGCCCTCAACAACCACCGGGAGGTGGCCCAGGTCCTAATCCGAGAGGGTC GCTGTGATGTGAATGTGCGGAACCGGAAGCTTCAGTCTCCACTTCATCTGGCTGTACAGCAGGCCCACCT GGGGCTGGTACCGCTGCTAGTGGACGCAGGCTGCAGTGTCAACACGGAGGACGAGGAGGGTGACACGGCC CTGCATGTGGCACTACAGCGTCACCAGCTGTTGCCCCTTGTGGCTGACAGGGCTGGGGGAGACCCAGGGC CCTTGCAGCTGCTGTCAAGGCTACAGGCCTCAGGCCTCCCAGGCTGCACAGAGTTGACTGTAGGCGCTGC AGTGGCCTGCTTCCTGGCGTTGGAGGGTGCTGATGTGAGCTACGCAAACCACCGCGGCCGGAGCCCACTG GACCTGGCCACAGAGGGCCGAGTGCTCAAGGCCTTGCAGGGCTGTGCCCAGCGCTTCAGGGAGCGACAGG CAGGTGGCGGTGGGGGTGTGCCCCCGGGCCCCCGGCATGTGCTGAGTACTCCTAACACCGTGACGAACCT GCACGTGTCTGGCACGGCAGGGCCAGAAGCTGCCGAGTGCCTGGTGTGCTCGGAGCTGGCATTGCTAATT CTGTTCTCACCGTGTCAGCACCGCACAGTGTGTGAGGAGTGCGCTCGAAGGATGAAGAAGTGTATCAGGT GCCAGGTGGTCATCAGCAAGAAGCTCCGCCCAGACGGCTCAGAGGTGGTAAATGCCATCCAGGTGCCCGG CCCGCCTCGGCAACTGGTGGAGGAGCTACAGAGCCGCTACAGGCAGATGGAGGAGCGCATCACCTGCCCC ATCTGCATCGACAGCCACATCCGCCTGGTGTTCCAGTGCGGCCATGGAGCGTGCGCGCCCTGTGGCGCCG CGCTTAACGCCTGCCCCATCTGCCGCCAGCCCATCCGCGACCGCATTCAGATCTTCGTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001256107
Insert Size	2862 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001256107.1, NP_001243036.1
RefSeq Size	4019 bp
RefSeq ORF	2862 bp
Locus ID	76580
Gene Summary	E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1), as well as variant 4, encodes isoform 1.

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