Mib2 (NM_001256107) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2210008I11Rik; skd; Zzank1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231425 representing NM_001256107 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACCTGGACCCCCATGCAGGTGTGCAGGTGGGCATGCGTGTGGTACGCGGAATGGACTGGAAATGGG GCCAGCAGGATGGAGGTGAGGGCGGTGTGGGCACCGTGGTGGAGCTTGGCCGCCATGGCAGCCCCTCGAC CCCCGACCGTACAGTTGTCGTTCAGTGGGACCAGGGCACACGTACCAACTATCGAGCTGGCTACCAAGGT GCCCATGACCTGTTGCTCTATGACAACGCCCAAATCGGTATCCGCCACCCCAATATCATCTGTGACTGCT GCAAGAAACATGGGCTTCGGGGCATGCGTTGGAAGTGCCGCGTCTGCTTTGACTATGACCTCTGCACACA GTGCTACATGCACAACAAGCATGACCTGACCCATGCCTTCGAGCGCTATGAGACATCTCACTCACGCCCG GTTACGCTGAGCCCCCGACAGGGCCTCCCTCGGATCCCACTGAGGGGCATCTTTCAAGGAGCTAAGGTGG TACGAGGCCCTGACTGGGAATGGGGCTCACAAGATGGAGGGGAAGGCAAGACAGGTCGTGTGGTGGACAT CCGTGGCTGGGATGTGGAGACAGGCCGAAGTGTGGCCAGTGTGACGTGGGCAGATGGAACCACGAACGTG TACCGAGTGGGCCACAAGGGCAAGGTGGATCTCAGATGTGTTGGTGAGGCAGCTGGCGGCTTTTACTACA AGGAGCACCTCCCAAAGCTTGGCAAGCCAGCAGAGCTGCAGCGCAGGGTGAGTGCTGATGGCCAGCCCTT CCAGCGTGGGGACAAGGTCAAGTGTCTGCTGGACACAGATGTCCTAAGGGATATGCAAGAAGGCCATGGT GGCTGGAACCCTAGGATGGCAGAGATGGGCACCGTGCACCGCATCACAGACCGTGGGGACGTGCGTGTGC AGTTCAACCACGAGACCCGCTGGACCTTCCACCCTGGGGCTCTCACCAAGCACAACAGCTTCTGGGTGGG TGATGTGGTCCGGGTCATCGGTGACCTTGACACTGTGAAGCGACTTCAGGCCGGACATGGTGAATGGACC GACGACATGGCTCCCGCCCTGGGCCGAGTGGGGAAAGTGGTGAAGGTGTTTGGAGATGGAAACTTGCGTG TGGCGGTTGGCGGTCAGCGGTGGACCTTCAGCCCCTCTTGCCTAGTGGCCTACCGGCCTGAGGAAGACGC CAACCTGGATGTGGCTGAGCGTGCCAGAGAGAACAAAAGCTCACTGAGTGTGGCCCTGGACAAGCTTCGG ACCCAGAAGAGTGACCCAGAGCACCCGGGGAGGCTGGTAGTAGAGGCCGCACTAGGAAATGTAGCCCGGG CTCTAGATCTTCTGCGGAGGCATCCAGAGCAGGTGGACACCAAGAACCAGGGTAGAACTGCCCTGCAGGT GGCTGCTTACCTGGGCCAGGTAGAGCTGGTGCGGCTGCTGCTGCAGGCAAGAGCAAGTATGGACCTGCCG GATGATGAGGGCAACACTGTGCTGCACTACACAGCCATGGGGAACCAGCCTGAGGCCACAAGGGTGCTCC TGAGTGCAGGGTGTGCAGTGGATGCTCGGAATGGCACACGCAGCACAGCCCTACATGTGGCTGTACAGAG GGGCTTCCTAGAGGTGGTAAAGATCCTGTGTGAACGTGGTTGTGATGTCAACTTGCCGGATGCGCATGCA GACACACCCCTGCATTCTGCCATCTCTGCTGGTGCTGGTGCCAGCAGCATTGTTGAAGTCCTCACCGAGG TCCCTGGCATCGATGTCACTGCTACCAATAGCCAGGGCTTCACACTGCTACACCATGCATCCCTCAAGGG CCATGTGCTAGCAGTCAGGAAGATTCTGGCAAGGGCCCGGCAGCTGGTGGATGCCAAGAAGGAGGATGGC TTTACCGCACTGCATCTGGCAGCCCTCAACAACCACCGGGAGGTGGCCCAGGTCCTAATCCGAGAGGGTC GCTGTGATGTGAATGTGCGGAACCGGAAGCTTCAGTCTCCACTTCATCTGGCTGTACAGCAGGCCCACCT GGGGCTGGTACCGCTGCTAGTGGACGCAGGCTGCAGTGTCAACACGGAGGACGAGGAGGGTGACACGGCC CTGCATGTGGCACTACAGCGTCACCAGCTGTTGCCCCTTGTGGCTGACAGGGCTGGGGGAGACCCAGGGC CCTTGCAGCTGCTGTCAAGGCTACAGGCCTCAGGCCTCCCAGGCTGCACAGAGTTGACTGTAGGCGCTGC AGTGGCCTGCTTCCTGGCGTTGGAGGGTGCTGATGTGAGCTACGCAAACCACCGCGGCCGGAGCCCACTG GACCTGGCCACAGAGGGCCGAGTGCTCAAGGCCTTGCAGGGCTGTGCCCAGCGCTTCAGGGAGCGACAGG CAGGTGGCGGTGGGGGTGTGCCCCCGGGCCCCCGGCATGTGCTGAGTACTCCTAACACCGTGACGAACCT GCACGTGTCTGGCACGGCAGGGCCAGAAGCTGCCGAGTGCCTGGTGTGCTCGGAGCTGGCATTGCTAATT CTGTTCTCACCGTGTCAGCACCGCACAGTGTGTGAGGAGTGCGCTCGAAGGATGAAGAAGTGTATCAGGT GCCAGGTGGTCATCAGCAAGAAGCTCCGCCCAGACGGCTCAGAGGTGGTAAATGCCATCCAGGTGCCCGG CCCGCCTCGGCAACTGGTGGAGGAGCTACAGAGCCGCTACAGGCAGATGGAGGAGCGCATCACCTGCCCC ATCTGCATCGACAGCCACATCCGCCTGGTGTTCCAGTGCGGCCATGGAGCGTGCGCGCCCTGTGGCGCCG CGCTTAACGCCTGCCCCATCTGCCGCCAGCCCATCCGCGACCGCATTCAGATCTTCGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231425 representing NM_001256107 Red=Cloning site Green=Tags(s) MDLDPHAGVQVGMRVVRGMDWKWGQQDGGEGGVGTVVELGRHGSPSTPDRTVVVQWDQGTRTNYRAGYQG AHDLLLYDNAQIGIRHPNIICDCCKKHGLRGMRWKCRVCFDYDLCTQCYMHNKHDLTHAFERYETSHSRP VTLSPRQGLPRIPLRGIFQGAKVVRGPDWEWGSQDGGEGKTGRVVDIRGWDVETGRSVASVTWADGTTNV YRVGHKGKVDLRCVGEAAGGFYYKEHLPKLGKPAELQRRVSADGQPFQRGDKVKCLLDTDVLRDMQEGHG GWNPRMAEMGTVHRITDRGDVRVQFNHETRWTFHPGALTKHNSFWVGDVVRVIGDLDTVKRLQAGHGEWT DDMAPALGRVGKVVKVFGDGNLRVAVGGQRWTFSPSCLVAYRPEEDANLDVAERARENKSSLSVALDKLR TQKSDPEHPGRLVVEAALGNVARALDLLRRHPEQVDTKNQGRTALQVAAYLGQVELVRLLLQARASMDLP DDEGNTVLHYTAMGNQPEATRVLLSAGCAVDARNGTRSTALHVAVQRGFLEVVKILCERGCDVNLPDAHA DTPLHSAISAGAGASSIVEVLTEVPGIDVTATNSQGFTLLHHASLKGHVLAVRKILARARQLVDAKKEDG FTALHLAALNNHREVAQVLIREGRCDVNVRNRKLQSPLHLAVQQAHLGLVPLLVDAGCSVNTEDEEGDTA LHVALQRHQLLPLVADRAGGDPGPLQLLSRLQASGLPGCTELTVGAAVACFLALEGADVSYANHRGRSPL DLATEGRVLKALQGCAQRFRERQAGGGGGVPPGPRHVLSTPNTVTNLHVSGTAGPEAAECLVCSELALLI LFSPCQHRTVCEECARRMKKCIRCQVVISKKLRPDGSEVVNAIQVPGPPRQLVEELQSRYRQMEERITCP ICIDSHIRLVFQCGHGACAPCGAALNACPICRQPIRDRIQIFV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001256107
ORF Size	2859 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001256107.1, NP_001243036.1
RefSeq Size	4019 bp
RefSeq ORF	2862 bp
Locus ID	76580
MW	104.5 kDa
Gene Summary	E3 ubiquitin-protein ligase that mediates ubiquitination of Delta receptors, which act as ligands of Notch proteins. Positively regulates the Delta-mediated Notch signaling by ubiquitinating the intracellular domain of Delta, leading to endocytosis of Delta receptors.[UniProtKB/Swiss-Prot Function]

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