Ddx46 (NM_001282055) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	2200005K02Rik; 8430438J23Rik; AI325430; AI957095; mKIAA0801
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229291 representing NM_001282055 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCCGGGAGTCTCGCCACTACCGAAAACGCTCAGCATCTCGAGGTCGTTCTGGAAGTCGATCGAGGA GTCGCTCACCCTCTGACAAAAGAAGCAAACGTGGGGATGACAGACGCTCTCGGAGCAGAGACAGAGACCG GAGGAGAGAGAGGTCTCGGAGTAGGGACAAGAGAAGATCTCGGTCAAGGGACAGGAAGCGCCTGAGGCGC TCCAGAAGTAGAGAGCGAGACAGAAGCCGAGAGCGGAGAAGATCTCGGAGCAGAGATAGAAGGCGCTCAA GGAGTCGAAGCCGGGGCAGGCGGTCCCGGTCCTCCAGTCCTGGCAGCAAAACCAAGAAGACTGAGAACAG GTCTAGGTCCAAAGAGAAAGCAGAAGGTGGGGACAGCTCCAAGGAGAAGAAAAAGGACAAGGATGACAAG GAGGACGAGAAAGAGAAGGATGCTGGGAACTTTGACCAGAATAAACTGGAAGAAGAAATGAGAAAACGGA AAGAAAGAGTTGAAAAATGGCGCGAAGAGCAGCGTAAGAAGGCTATGGAGAACATAGGAGAGCTGAAGAA GGAGATTGAGGAGATGAAGCAGGGGAAGAAGTGGAGCTTGGAGGACGACGACGATGATGAAGATGATCCT GCCGAGGCTGAGAAGGAAGGGACTGAAATGGAGGACGAGGAGCTGGACCCATTAGACGCCTACATGGAAG AAGTGAAGGAAGAGGTAAAAAAGTTTAACATGAGGAGCGTGAAAGGCGGCGCAGGAAATGAGAAGAAGTC TGGGCCAACAGTCACAAAAGTTGTCACTGTGGTGACCACCAAGAAAGCAGTAGTAGACGCGGATAAGAAG AAGGGGGAACTCATGGAGAATGACCAGGACGCCATGGAGTATTCCTCAGAGGAGGAGGAAGTTGACCTTC AAACGGCCCTCACAGGCTACCAGACAAAACAGAGAAAACTTCTAGAGCCAGTTGATCATGGAAAAATTGA ATATGAGCCATTCAGAAAAAACTTTTATGTTGAAGTTCCAGAATTAGCAAAAATGTCTCAAGAAGAGGTG AATGTGTTTCGATTGGAAATGGAGGGCATTACAGTTAAAGGAAAAGGTTGCCCGAAACCAATTAAATCAT GGGTCCAGTGTGGAATTTCCATGAAGATACTAAATTCCCTCAAAAAGCATGGCTATGAAAAGCCAACACC AATCCAAACTCAAGCGATTCCTGCTATAATGTCTGGACGAGATTTGATTGGCATTGCCAAGACAGGAAGT GGAAAAACCATTGCTTTTCTTTTGCCCATGTTTAGACACATCATGGATCAGAGGTCATTAGAAGAAGGAG AGGGACCAATAGCTGTCATCATGACTCCAACTCGAGAGCTGGCTTTACAAATCACTAAAGAATGTAAGAA GTTTTCGAAGACCTTGGGACTGAGAGTGGTCTGTGTGTATGGAGGGACAGGAATCAGCGAGCAGATTGCT GAGCTGAAAAGAGGTGCTGAAATTATTGTTTGCACACCTGGTCGAATGATTGACATGTTAGCGGCTAACA GTGGTCGGGTCACAAATCTTCGAAGAGTGACATATGTTGTTTTAGATGAAGCAGACAGAATGTTTGACAT GGGTTTTGAACCACAGGTCATGCGCATTGTGGATAACGTTCGTCCTGACCGACAGACAGTTATGTTTTCA GCCACTTTCCCTAGAGCTATGGAGGCTTTGGCCCGCAGGATCCTGAGCAAACCTATTGAAGTGCAGGTTG GAGGCAGGAGTGTGGTTTGCTCCGATGTGGAACAGCAGGTGATTGTGATTGAAGAAGAAAAGAAATTCTT GAAATTGCTGGAGCTTTTAGGCCATTATCAGGAGTCAGGATCTGTCATTATATTTGTGGATAAGCAGGAA CACGCAGATGGTCTTCTGAAGGACTTGATGAGAGCATCGTACCCTTGCATGTCTCTTCACGGAGGTATTG ATCAATATGACAGGGACAGTATCATAAATGACTTTAAGAATGGAACCTGCAAGCTCCTCGTTGCCACATC TGTTGCAGCTCGTGGTCTCGATGTGAAACATCTAATTCTTGTAGTGAATTACAGCTGCCCCAATCATTAT GAAGATTATGTGCACAGAGCTGGGCGGACTGGAAGAGCGGGCAACAAGGGTTATGCCTACACCTTCATCA CAGAGGATCAAGCTCGATATGCTGGTGACATCATTAAAGCTCTTGAATTGTCAGGGACCGCAGTGCCTCC TGACCTAGAAAAACTCTGGAGTGACTTCAAAGATCAACAGAAAGCTGAAGGAAAAATAATTAAAAAGAGT AGTGGCTTCTCTGGCAAGGGATTCAAGTTTGATGAAACAGAACAGGCTTTAGCCAATGAGAGGAAAAAGC TGCAGAAAGCTGCTCTTGGCCTGCAAGATTCTGATGACGAGGATGCTGCAGTTGATATTGATGAACAGAT TGAAAGCATGTTTAATTCAAAGAAGAGAGTAAAGGACATGGCTGCACCCGGCACATCCAGCGTGCCCGCG CCCACTGCAGGCAATGCTGAGAAGCTAGAAATCGCCAAGAGATTGGCTCTCAGAATCAACGCTCAGAAGA ACCTGGGCATCGAGTCTCAGGTAGATGTGATGCAGCAGGCCACCAACGCCATCCTCAGAGGTGGCACTAT CCTGGCTCCCACTGTGTCTGCGAAAACCATTGCAGAGCAGCTTGCTGAGAAGATCAATGCCAAGCTCAAC TACGTGCCTTTGGAGAAGCAGGAGGAAGAGAGACAGGAGGGTGGGCAGAGCGAGTCTTTCAAGAGATACG AGGAAGAATTAGAAATTAATGACTTCCCACAGACTGCGAGGTGGAAAGTCACCTCAAAGGAAGCCCTGCA GAGGATCAGCGAGTACTCTGAGGCCGCCATCACCATCAGGGGCACCTACTTCCCTCCCGGCAAGGAGCCC AAGGAAGGAGAGCGCAAGATCTACCTGGCCATCGAAAGTGCCAACGAGCTGGCTGTGCAGAAAGCAAAGG CAGAGATCACCAGGCTCATAAAAGAGGAACTGATCCGACTGCAAAATTCATACCAACCAACCAACAAAGG ACGATACAAAGTATTATAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001282055
Insert Size	3099 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001282055.1, NP_001268984.1
RefSeq Size	5715 bp
RefSeq ORF	3099 bp
Locus ID	212880
UniProt ID	Q569Z5
Gene Summary	Plays an essential role in splicing, either prior to, or during A complex formation.[UniProtKB/Swiss-Prot Function]

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