Tsc1 (NM_001289576) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229419 representing NM_001289576 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGTTAGCCAACATTGGGGAGCTGCTCTCCATGCTGGACTCCTCCACACTGGGTGTGCGGGATG ACGTGACAGCCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTCAACACGTTGGTTGA TTATTACCTGGAAACCAATTCTCAGCCGGTATTGCACATCCTGACCACCTTGCAGGAGCCACACGATAAG CACCTCTTGGACAAAATTAATGAGTATGTAGGCAAAGCTGCTACCCGTTTATCCATCCTCTCGCTGCTGG GGCATGTTGTGAGACTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTATT GAAATGTCTCAAGATGGACACTGATGTTGTGGTCCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTC CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTCTCGTCAT GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTGTACCTGGTCCATCTTCATGCCAGTGTTTATGCCCT CTTTCACCGCCTTTATGGGATGTACCCATGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGTATGAAG GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT AGAGTGTGCCAAAATCTCTCTGGACCCTACAGAAGCCTCGTATGAAGATGGCTATTCTGTGTCACACCAG CTCTCTGCTTGCTTCCCTTACCGTTCAGCTGATGTCACCACCAGCCCTTATGTGGACACACAGAATAGCT ATGGGGGTTCTACTTCCACCCCTTCCTCCAGCTCTCGGCTGATGTTGTTCAGTCCACCTGGGCAGCTACC TCAGAGTTTGAGTTCCCCATCAACACGGCTGTTACCTGAGCCGCTGCAAGCTAGTCTCTGGAGCCCATCT GCGGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCAGCTGATTTGTCACATCCGTATA GTAAAGCCTTTGGTACCACTGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCTCCAGC CCCACCTTGTCCCCAAGATGACTGTGTGCATGGTTCAGCAGCCCAGGCCTCAGCCACAGCCCCCAGGAAG GAAGAAAGAGCAGATTCCTCAAGGCCTTACCTTCACAGACAGTCAAACGACCGAGGATTAGAGGATCCAC CTGGAAGCAAAGGTTCCGTTACTCTGAGGAATCTACCTGATTTCCTAGGTGATCTGGCTTCTGAGGAAGA CAGTATCGAGAAAGATAAGGAAGAAGCTGCAATATCTAAAGAGCTTTCTGAGATCACTACTGCAGAGGCG GATCCTGTAGTTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTGGCTCTCAGCGGA AGACTCATTCGGCAGCCTCTGGGACTCAGGGCTCCAGCGTGAACCCTGAGCCTTTGCACTCCTCCCTGGA CAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGCAGTGCTGATGTC AGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCAGCCCTTGCAAAA TCCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCTCTTTGAGGTGGC CTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAGAAGACTGAGGAGCTGTTGAAGAAAGTGAAAGGAAAC CCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGATAGAGCAGGGAGCAG GTGCGCACAGCAAGGAGCTGAGCAGGTTGTCCCTGCCCAGCAAGTCTGTTGACTGGACCCACTTTGGAGA TGAGCTCCGGACCCTCCGAGACCAGTTGCTTTTACTGCACAATCAGCTGCTGTACGAGCGCTTCAAGCGG CAGCAGCATGCACTCAGGAACAGAAGGCTCCTGCGCAAGGTGATCCGAGCAGCGGCTCTGGAGGAACACA ATGCAGCAATGAAAGATCAGTTGAAGTTACAAGAGAAGGACATACAGATGTGGAAGGTGAGTCTGCAGAA AGAACAAGCCCGATACAGTCAGCTTCAGGAACAACGTGACACCATGGTGACCCAACTGCATAGCCAGATC AGACAGCTACAGCATGACCGAGAAGAATTCTACAACCAGAGTCAGGAGTTACAGACAAAGCTGGAGGACT GCAGAAACATGATTGCGGAGCTTCGGGTGGAGCTGAAGAAGGCTAACAACAAGGTGTGCCACACTGAGCT GCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAACAGTGAGTCAGTGCAGCAGCAGATGGAGTTCTTGAAT AGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGGAGCAGCTGCAGAGCAAGCATCCTGACA CCACCAAGGAAGTAGAAATGATGAAAACTGCATATCGGAAAGAGCTAGAGAAAAACAGAAGCCACCTTCT CCAGCAGAACCAAAGGTTGGACGCCTCACAGAGGCGAGTTTTGGAACTGGAGTCTCTTCTGGCCAAGAAA GACCACCTTCTCCTAGAACAGAAGAAATATCTTGAGGATGTCAAGAGCCAGGCGAGTGGACAGCTGCTGG CTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGTGTTGGAACTGGAGATCCTAGACTTGTA TGGCAGGTTGGAAAAAGATGGCCGCCTACGGAAACTAGAAGAGGACAGAGCAGAGGCAGCAGAGGCAGCA GAAGAGAGGCTTGACTGTTGTAGTGATGGATGCACAGATTCCTTGGTAGGACATAATGAAGAGGCTTCTG GTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCGGGCCAGCTGTGGAGGTAGAGTCACTGG AGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAACCCCCGAGCCAGAGGTTCAGCAGCCGG TGGGAACCTGCCCTGGGCGAGCCCTCCAGCAGCATCCCCACCACTGTTGGCTCACTTCCCAGTTCCAAAA GCTTCCTGGGCATGAAGGCCCGGGAGCTGTTCCGTAATAAGAGCGAGAGCCAGTGTGATGAGGACAGCGT GACCATGAGTAGCAGCAGCCTTTCTGAGACCCTGAAGACAGAACTGGGCAAGGACTCGGGCACAGAAAAC AAGACTTCCCTGAGTCTAGATGCCCCACACCCATCTTCCCCAAACTCAGACAATGTGGGGCAGCTCCACA TCATGGACTACAATGAGACTCATCCTGAACACAGCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001289576
Insert Size	3468 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289576.1, NP_001276505.1
RefSeq Size	7714 bp
RefSeq ORF	3468 bp
Locus ID	64930
UniProt ID	Q9EP53
Gene Summary	In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) uses two alternate in-frame splice sites, one each in the 5' and 3' coding regions, compared to variant 1. It encodes isoform 3, which is shorter than isoform 1.

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