Tsc1 (NM_001289576) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231630 representing NM_001289576 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGTTAGCCAACATTGGGGAGCTGCTCTCCATGCTGGACTCCTCCACACTGGGTGTGCGGGATG ACGTGACAGCCATCTTCAAGGAGTCCCTCAATTCTGAACGTGGGCCTATGCTTGTCAACACGTTGGTTGA TTATTACCTGGAAACCAATTCTCAGCCGGTATTGCACATCCTGACCACCTTGCAGGAGCCACACGATAAG CACCTCTTGGACAAAATTAATGAGTATGTAGGCAAAGCTGCTACCCGTTTATCCATCCTCTCGCTGCTGG GGCATGTTGTGAGACTGCAGCCATCTTGGAAGCATAAGCTCTCTCAAGCACCTCTTCTGCCTTCTTTATT GAAATGTCTCAAGATGGACACTGATGTTGTGGTCCTCACAACTGGTGTCTTGGTGTTGATCACCATGCTC CCGATGATCCCGCAGTCAGGGAAGCAGCACCTTCTCGACTTCTTTGACATCTTTGGCCGTCTCTCGTCAT GGTGCCTGAAGAAACCAGGCCATGTGACAGAAGTGTACCTGGTCCATCTTCATGCCAGTGTTTATGCCCT CTTTCACCGCCTTTATGGGATGTACCCATGTAACTTCGTCTCCTTCCTGCGCTCTCACTACAGTATGAAG GAAAACGTGGAGACTTTTGAAGAAGTGGTCAAGCCAATGATGGAGCATGTGCGAATTCACCCGGAATTAG TGACTGGATCCAAGGACCATGAACTGGACCCTCGAAGGTGGAAGACATTAGAAACTCATGATGTTGTAAT AGAGTGTGCCAAAATCTCTCTGGACCCTACAGAAGCCTCGTATGAAGATGGCTATTCTGTGTCACACCAG CTCTCTGCTTGCTTCCCTTACCGTTCAGCTGATGTCACCACCAGCCCTTATGTGGACACACAGAATAGCT ATGGGGGTTCTACTTCCACCCCTTCCTCCAGCTCTCGGCTGATGTTGTTCAGTCCACCTGGGCAGCTACC TCAGAGTTTGAGTTCCCCATCAACACGGCTGTTACCTGAGCCGCTGCAAGCTAGTCTCTGGAGCCCATCT GCGGTCTGTGGTATGACCACTCCTCCTACGTCTCCTGGAAATGTCCCAGCTGATTTGTCACATCCGTATA GTAAAGCCTTTGGTACCACTGGTGGAAAAGGAACTCCTTCAGGAACCCCAGCGACCTCTCCTCCTCCAGC CCCACCTTGTCCCCAAGATGACTGTGTGCATGGTTCAGCAGCCCAGGCCTCAGCCACAGCCCCCAGGAAG GAAGAAAGAGCAGATTCCTCAAGGCCTTACCTTCACAGACAGTCAAACGACCGAGGATTAGAGGATCCAC CTGGAAGCAAAGGTTCCGTTACTCTGAGGAATCTACCTGATTTCCTAGGTGATCTGGCTTCTGAGGAAGA CAGTATCGAGAAAGATAAGGAAGAAGCTGCAATATCTAAAGAGCTTTCTGAGATCACTACTGCAGAGGCG GATCCTGTAGTTCCTCGAGGGGGCTTTGACTCTCCCTTCTACCGAGACAGTCTCTCTGGCTCTCAGCGGA AGACTCATTCGGCAGCCTCTGGGACTCAGGGCTCCAGCGTGAACCCTGAGCCTTTGCACTCCTCCCTGGA CAAACATGGGCCTGACACACCAAAGCAAGCCTTTACTCCCATAGACCCACCCTCTGGCAGTGCTGATGTC AGTCCCGCTGGGGACAGGGATCGCCAGACTTCTCTGGAGACCAGTATCCTCACTCCCAGCCCTTGCAAAA TCCCACCTCAGAGGGGAGTGAGCTTTGGAAGTGGGCAGCTTCCCCCATATGATCATCTCTTTGAGGTGGC CTTGCCAAAGACTGCCTGTCACTTTGTCAGCAAGAAGACTGAGGAGCTGTTGAAGAAAGTGAAAGGAAAC CCTGAGGAAGACTGTGTGCCCTCTACCTCCCCAATGGAAGTACTGGACAGACTGATAGAGCAGGGAGCAG GTGCGCACAGCAAGGAGCTGAGCAGGTTGTCCCTGCCCAGCAAGTCTGTTGACTGGACCCACTTTGGAGA TGAGCTCCGGACCCTCCGAGACCAGTTGCTTTTACTGCACAATCAGCTGCTGTACGAGCGCTTCAAGCGG CAGCAGCATGCACTCAGGAACAGAAGGCTCCTGCGCAAGGTGATCCGAGCAGCGGCTCTGGAGGAACACA ATGCAGCAATGAAAGATCAGTTGAAGTTACAAGAGAAGGACATACAGATGTGGAAGGTGAGTCTGCAGAA AGAACAAGCCCGATACAGTCAGCTTCAGGAACAACGTGACACCATGGTGACCCAACTGCATAGCCAGATC AGACAGCTACAGCATGACCGAGAAGAATTCTACAACCAGAGTCAGGAGTTACAGACAAAGCTGGAGGACT GCAGAAACATGATTGCGGAGCTTCGGGTGGAGCTGAAGAAGGCTAACAACAAGGTGTGCCACACTGAGCT GCTGCTCAGCCAGGTCTCTCAGAAGCTCTCCAACAGTGAGTCAGTGCAGCAGCAGATGGAGTTCTTGAAT AGGCAGCTCCTGGTGCTCGGGGAGGTCAATGAGCTGTACCTGGAGCAGCTGCAGAGCAAGCATCCTGACA CCACCAAGGAAGTAGAAATGATGAAAACTGCATATCGGAAAGAGCTAGAGAAAAACAGAAGCCACCTTCT CCAGCAGAACCAAAGGTTGGACGCCTCACAGAGGCGAGTTTTGGAACTGGAGTCTCTTCTGGCCAAGAAA GACCACCTTCTCCTAGAACAGAAGAAATATCTTGAGGATGTCAAGAGCCAGGCGAGTGGACAGCTGCTGG CTGCAGAGAGCAGGTATGAGGCTCAGAGAAAGATCACCCGGGTGTTGGAACTGGAGATCCTAGACTTGTA TGGCAGGTTGGAAAAAGATGGCCGCCTACGGAAACTAGAAGAGGACAGAGCAGAGGCAGCAGAGGCAGCA GAAGAGAGGCTTGACTGTTGTAGTGATGGATGCACAGATTCCTTGGTAGGACATAATGAAGAGGCTTCTG GTCACAATGGTGAGACCAGGACCTCCAGACCTGGTGGCACCCGGGCCAGCTGTGGAGGTAGAGTCACTGG AGGCAGCAGCAGCAGCAGCAGTGAGCTTTCCACTCCAGAGAAACCCCCGAGCCAGAGGTTCAGCAGCCGG TGGGAACCTGCCCTGGGCGAGCCCTCCAGCAGCATCCCCACCACTGTTGGCTCACTTCCCAGTTCCAAAA GCTTCCTGGGCATGAAGGCCCGGGAGCTGTTCCGTAATAAGAGCGAGAGCCAGTGTGATGAGGACAGCGT GACCATGAGTAGCAGCAGCCTTTCTGAGACCCTGAAGACAGAACTGGGCAAGGACTCGGGCACAGAAAAC AAGACTTCCCTGAGTCTAGATGCCCCACACCCATCTTCCCCAAACTCAGACAATGTGGGGCAGCTCCACA TCATGGACTACAATGAGACTCATCCTGAACACAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231630 representing NM_001289576 Red=Cloning site Green=Tags(s) MAQLANIGELLSMLDSSTLGVRDDVTAIFKESLNSERGPMLVNTLVDYYLETNSQPVLHILTTLQEPHDK HLLDKINEYVGKAATRLSILSLLGHVVRLQPSWKHKLSQAPLLPSLLKCLKMDTDVVVLTTGVLVLITML PMIPQSGKQHLLDFFDIFGRLSSWCLKKPGHVTEVYLVHLHASVYALFHRLYGMYPCNFVSFLRSHYSMK ENVETFEEVVKPMMEHVRIHPELVTGSKDHELDPRRWKTLETHDVVIECAKISLDPTEASYEDGYSVSHQ LSACFPYRSADVTTSPYVDTQNSYGGSTSTPSSSSRLMLFSPPGQLPQSLSSPSTRLLPEPLQASLWSPS AVCGMTTPPTSPGNVPADLSHPYSKAFGTTGGKGTPSGTPATSPPPAPPCPQDDCVHGSAAQASATAPRK EERADSSRPYLHRQSNDRGLEDPPGSKGSVTLRNLPDFLGDLASEEDSIEKDKEEAAISKELSEITTAEA DPVVPRGGFDSPFYRDSLSGSQRKTHSAASGTQGSSVNPEPLHSSLDKHGPDTPKQAFTPIDPPSGSADV SPAGDRDRQTSLETSILTPSPCKIPPQRGVSFGSGQLPPYDHLFEVALPKTACHFVSKKTEELLKKVKGN PEEDCVPSTSPMEVLDRLIEQGAGAHSKELSRLSLPSKSVDWTHFGDELRTLRDQLLLLHNQLLYERFKR QQHALRNRRLLRKVIRAAALEEHNAAMKDQLKLQEKDIQMWKVSLQKEQARYSQLQEQRDTMVTQLHSQI RQLQHDREEFYNQSQELQTKLEDCRNMIAELRVELKKANNKVCHTELLLSQVSQKLSNSESVQQQMEFLN RQLLVLGEVNELYLEQLQSKHPDTTKEVEMMKTAYRKELEKNRSHLLQQNQRLDASQRRVLELESLLAKK DHLLLEQKKYLEDVKSQASGQLLAAESRYEAQRKITRVLELEILDLYGRLEKDGRLRKLEEDRAEAAEAA EERLDCCSDGCTDSLVGHNEEASGHNGETRTSRPGGTRASCGGRVTGGSSSSSSELSTPEKPPSQRFSSR WEPALGEPSSSIPTTVGSLPSSKSFLGMKARELFRNKSESQCDEDSVTMSSSSLSETLKTELGKDSGTEN KTSLSLDAPHPSSPNSDNVGQLHIMDYNETHPEHS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289576
ORF Size	3465 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289576.1, NP_001276505.1
RefSeq Size	7714 bp
RefSeq ORF	3468 bp
Locus ID	64930
UniProt ID	Q9EP53
MW	128.7 kDa
Gene Summary	In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function]

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