Tjp2 (NM_001198985) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	ZO-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229457 representing NM_001198985 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGCGGGGAGACCGCGGGTTCCCACCGCGGCGGGAGCTGTCGGGCTGGCAGCGCGGTCCAGGCA TGGAGGAGGTGATATGGGAGCAGTACACCGTGACCCTGCAGAAGGATTCCAAAAGAGGATTTGGAATTGC CGTGTCGGGAGGCAGAGACAACCCCCACTTTGAAAATGGGGAGACGTCAATCGTCATCTCAGATGTGCTC CCAGGTGGACCCGCTGATGGCTTGCTTCAAGAAAATGACAGGGTGGTCATGGTCAATGGCACCCCCATGG AAGATGTGCTCCATTCGTTTGCCGTTCAGCAGCTTAGGAAAAGTGGGAAGATAGCAGCCATCGTGGTCAA GAGACCCCGGAAGGTTCAGGTGGCCCCACTGCAAGGCAGCCCCCCTCTCAGTCATGACGACCGAGGTTTT GAAGTGATTGAAGAATTTGATGGCAGAAGTTTCCGCAGCGGCTACAGTGAGAGGAGTCGGCACAGCAGCC ACGACATGCTCAGCCACAGCTGGGAGGGCAATAGGGAGAGGGGGCGACCCCATCAGCGCACACAGAGCCG GGAGCGGGAGCGCAGCCGTGGCAGGAGCCTGGAACGGGGCCTGGACCAGGAAGACTATGGGCGCAGCCGT GAGCGCAGCCGTGGCCGGAGCCTGGAGCGCGGGCTGGACCGCGACTTTGTGTCCAGGGACCACAGCCGTG GCCGTAGCATCGACAGGGACTACGACCGAGACTATGAGCGCTCCTATCACGAAGCTTATGAACCCGACTA CGGAGGCGGCTACAGCCCATCATATGACCGTAGAGCCCATCCAGAGACCCGCTATGAACGGAGCCGCAGC CGAGAACACCTACGTTCCCGAAGCCCCAGCCCCGAGTCTAGGTCACGGCATGAACACAAGGGCCAACATG ATCCCGACAGGCCCATCGGGGTCCTTCTAACCAAAAGCAAAGCAAATGAAGAGTATGGGCTCCGGCTGGG GAGTCAGATCTTCATCAAAGAAATGACCAGAACAGGACTGGCCACCAAAGATGGCAACCTTCATGAGGGG GACATAATTCTCAAGATCAACGGCACTGTTACTGAGAACATGTCTCTAACGGATGCTCGGAAGTTAATAG AAAAGTCTCGAGGAAAACTGCAGCTTGTGGTGTTGAGAGACAGCAAGCAGACCCTCATCAACATCCCAGC CCTAAACGACAGCGACTCTGAAGTGGAGGATATCTCGGAAATAGAGTCCAACCGGTCTTTCTCTCCAGAG GAGAGACGCCAGCAGTATTCTGATCAGGATTACCATTCCTCCACTGAGAAGCTGAAGGAGAGGCCGAGCT CAAGAGAGGAGACCTCAGGCAGACTATCCAGGATGGGTGCCACTCCCACGCCGTTCAAGTCCACGGGGGA CATCACAGCTGCGGGGGTCACAGAGGCCAGCAGGGAGCCCAGGTACCAGGAAGAAGGCCCAGTTCCTCAA CCCAGAACAGCTCCAAGAGTTTTTCTTCGTCCTAGTCCCGAAGATGAAGCAATATATGGCCCTAACACCA AAATGGTGAGGTTCAAGAAGGGAGACAGCGTGGGCCTCCGGTTGGCTGGTGGAAACGACGTTGGGATATT TGTGGCTGGCATTCAGGAGGGCACCTCCGCAGAGCAGGAGGGCCTGCAAGAAGGAGACCAGATTCTGAAG GTGAACACACAGGATTTCAGAGGGCTAGTCCGGGAAGATGCGGTCCTCTACCTGCTAGAAATCCCCAAAG GTGAAACTGTGACCATTTTGGCTCAGAGCCGAGCAGACGTGTACAGAGATATCCTGGCTTGTGGCAGGGG AGATTCGTTTTTCATAAGAAGCCACTTTGAATGTGAGAAGGAAACTCCCCAGAGCCTGGCCTTCACCAGG GGAGAGGTCTTCCGAGTGGTAGACACGCTGTACGATGGCAAGCTGGGCCACTGGCTGGCCGTGAGGATCG GGAATGAGCTGGAGAAAGGCTTGATCCCTAACAAAAGCAGAGCCGAGCAAATGGCCAGTGTCCAGAATGC CCAGCGAGAGAACGCCGGGGACAGAGCAGACTTCTGGCGGATGCGTGGCCAGAGATCCAGCGGCGGCGTC AAGAAGAACCTCCGCAAGAGCCGGGAAGACCTGGCGGCTGCTGTATCGGTTAGCACCAAGTTCCCTGCCT ACGAGAAGGTTCTGCTTCGGGAAGCTGGCTTCAAGAGACCCGTGGTTCTGTTTGGCCCCATAGCAGATAT AGCAATGGAAAGGTTGGCAACTGAGTTACCAGACCTGTTTCAAACTGCAAAAACAGAACCCAAAGATGCC GGATCTGAGAAATCCAGTGGAGTGGTTCGGTTGAATACCGTGAGGCAAATTATTGAGCAGGACAAGCACG CCCTGCTCGACGTTACCCCCAAAGCTGTGGACCTGCTCAACTACACTCAGTGGTTCCCTATCGTGATTTT CTTCAACCCGGATTCCAGACAAGGTGTTAAAACCATAAGGCAGAGGTTGAGTCCAACATCCAATAAAAGT TCTCGCAAGTTATTTGATCAAGCCAACAAGCTCAAAAAGACCTGTTCTCATCTTTTTACAGCCACAATCA ACGTGAATTCAGCCAACGATGGCTGGTTTGGCAGCTTGAAGGACAGCATTCAGCAGCAGCAAAACGAAGC AGTCTGGGTCTCTGAAGGAAAGATGGAAGGGATGGATGATGATGCTGAAGACCGCATGTCCTACTTAACC GCCATGGGCGCGGACTATCTGAGTTGTGACAGCCGTCTCATCAGTGACTTTGAAGACACCGACGGCGAGG GAGGCGCCTACACTGACAATGAGCTAGAGGAGCCGGCGGAGGAGCCGCTGGTGTCTTCCATCACCCGCTC CTCGGAGCCGGTGCAGCACGAGGAGAACATAAGGAAATCCAGCCCAGAGCCACGAGCTCAGATGAGGAGG GCTGCTAGCAGAGACCAACTTAGGGATGCTAGCCCGCCCCCAGCCTTCAAGCCAGAGCCGCCCAAGGCAA GAAGCCAGAACAGAGAAGACTCCTTCGACTACTCCAAGTCAAACCTCCCCGCCACAGCCGGCAGTGAAAT CCCGGGGGGATCCACCAAAGGCTATCCTCCCCCTATTGCAGCGAAGCCTGCCTTTGGGCGGCCCATCCTG AAGCCTTCCACTCCAGTCCCTATGCCTGAGAGCGAGGAGGTTGGAGAGAGCACCGAGGAGCAGGAAGATG CTCCCAGATCAGTCCTGGGCAGAGTGAAAATATTCGAGAAGATGGACCACAAGGCAAAATTACAGAGGAT GCAGGAGCTCCAGGAGGCCCAGAATGCGAGGATCGAAATAGCTCAGAAGCACCCTGACATCTATGCGGTT CCAATCAAGGCCCCCAAGCCAGATGCTGGCCTCCCCCCGCACATGAGTTCTAGACCCCCAGAGCCCCAGA AAGCTCCTTCCAGACTTTACCAGGACACCAGAGGAAGCTACGGCAGTGACCCCGAGGAGGAGGAGTACCG CCAGCAGTTGGCAGCACACTCGAAGCGTGGTTACTACAGCCAGCCCTCCCGGTACCGAGACACGGAGTTA TAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001198985
Insert Size	3573 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001198985.1, NP_001185914.1
RefSeq Size	4637 bp
RefSeq ORF	3573 bp
Locus ID	21873
Gene Summary	Plays a role in tight junctions and adherens junctions.[UniProtKB/Swiss-Prot Function]

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