Tjp2 (NM_001198985) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	ZO-2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231668 representing NM_001198985 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGCGGGGAGACCGCGGGTTCCCACCGCGGCGGGAGCTGTCGGGCTGGCAGCGCGGTCCAGGCA TGGAGGAGGTGATATGGGAGCAGTACACCGTGACCCTGCAGAAGGATTCCAAAAGAGGATTTGGAATTGC CGTGTCGGGAGGCAGAGACAACCCCCACTTTGAAAATGGGGAGACGTCAATCGTCATCTCAGATGTGCTC CCAGGTGGACCCGCTGATGGCTTGCTTCAAGAAAATGACAGGGTGGTCATGGTCAATGGCACCCCCATGG AAGATGTGCTCCATTCGTTTGCCGTTCAGCAGCTTAGGAAAAGTGGGAAGATAGCAGCCATCGTGGTCAA GAGACCCCGGAAGGTTCAGGTGGCCCCACTGCAAGGCAGCCCCCCTCTCAGTCATGACGACCGAGGTTTT GAAGTGATTGAAGAATTTGATGGCAGAAGTTTCCGCAGCGGCTACAGTGAGAGGAGTCGGCACAGCAGCC ACGACATGCTCAGCCACAGCTGGGAGGGCAATAGGGAGAGGGGGCGACCCCATCAGCGCACACAGAGCCG GGAGCGGGAGCGCAGCCGTGGCAGGAGCCTGGAACGGGGCCTGGACCAGGAAGACTATGGGCGCAGCCGT GAGCGCAGCCGTGGCCGGAGCCTGGAGCGCGGGCTGGACCGCGACTTTGTGTCCAGGGACCACAGCCGTG GCCGTAGCATCGACAGGGACTACGACCGAGACTATGAGCGCTCCTATCACGAAGCTTATGAACCCGACTA CGGAGGCGGCTACAGCCCATCATATGACCGTAGAGCCCATCCAGAGACCCGCTATGAACGGAGCCGCAGC CGAGAACACCTACGTTCCCGAAGCCCCAGCCCCGAGTCTAGGTCACGGCATGAACACAAGGGCCAACATG ATCCCGACAGGCCCATCGGGGTCCTTCTAACCAAAAGCAAAGCAAATGAAGAGTATGGGCTCCGGCTGGG GAGTCAGATCTTCATCAAAGAAATGACCAGAACAGGACTGGCCACCAAAGATGGCAACCTTCATGAGGGG GACATAATTCTCAAGATCAACGGCACTGTTACTGAGAACATGTCTCTAACGGATGCTCGGAAGTTAATAG AAAAGTCTCGAGGAAAACTGCAGCTTGTGGTGTTGAGAGACAGCAAGCAGACCCTCATCAACATCCCAGC CCTAAACGACAGCGACTCTGAAGTGGAGGATATCTCGGAAATAGAGTCCAACCGGTCTTTCTCTCCAGAG GAGAGACGCCAGCAGTATTCTGATCAGGATTACCATTCCTCCACTGAGAAGCTGAAGGAGAGGCCGAGCT CAAGAGAGGAGACCTCAGGCAGACTATCCAGGATGGGTGCCACTCCCACGCCGTTCAAGTCCACGGGGGA CATCACAGCTGCGGGGGTCACAGAGGCCAGCAGGGAGCCCAGGTACCAGGAAGAAGGCCCAGTTCCTCAA CCCAGAACAGCTCCAAGAGTTTTTCTTCGTCCTAGTCCCGAAGATGAAGCAATATATGGCCCTAACACCA AAATGGTGAGGTTCAAGAAGGGAGACAGCGTGGGCCTCCGGTTGGCTGGTGGAAACGACGTTGGGATATT TGTGGCTGGCATTCAGGAGGGCACCTCCGCAGAGCAGGAGGGCCTGCAAGAAGGAGACCAGATTCTGAAG GTGAACACACAGGATTTCAGAGGGCTAGTCCGGGAAGATGCGGTCCTCTACCTGCTAGAAATCCCCAAAG GTGAAACTGTGACCATTTTGGCTCAGAGCCGAGCAGACGTGTACAGAGATATCCTGGCTTGTGGCAGGGG AGATTCGTTTTTCATAAGAAGCCACTTTGAATGTGAGAAGGAAACTCCCCAGAGCCTGGCCTTCACCAGG GGAGAGGTCTTCCGAGTGGTAGACACGCTGTACGATGGCAAGCTGGGCCACTGGCTGGCCGTGAGGATCG GGAATGAGCTGGAGAAAGGCTTGATCCCTAACAAAAGCAGAGCCGAGCAAATGGCCAGTGTCCAGAATGC CCAGCGAGAGAACGCCGGGGACAGAGCAGACTTCTGGCGGATGCGTGGCCAGAGATCCAGCGGCGGCGTC AAGAAGAACCTCCGCAAGAGCCGGGAAGACCTGGCGGCTGCTGTATCGGTTAGCACCAAGTTCCCTGCCT ACGAGAAGGTTCTGCTTCGGGAAGCTGGCTTCAAGAGACCCGTGGTTCTGTTTGGCCCCATAGCAGATAT AGCAATGGAAAGGTTGGCAACTGAGTTACCAGACCTGTTTCAAACTGCAAAAACAGAACCCAAAGATGCC GGATCTGAGAAATCCAGTGGAGTGGTTCGGTTGAATACCGTGAGGCAAATTATTGAGCAGGACAAGCACG CCCTGCTCGACGTTACCCCCAAAGCTGTGGACCTGCTCAACTACACTCAGTGGTTCCCTATCGTGATTTT CTTCAACCCGGATTCCAGACAAGGTGTTAAAACCATAAGGCAGAGGTTGAGTCCAACATCCAATAAAAGT TCTCGCAAGTTATTTGATCAAGCCAACAAGCTCAAAAAGACCTGTTCTCATCTTTTTACAGCCACAATCA ACGTGAATTCAGCCAACGATGGCTGGTTTGGCAGCTTGAAGGACAGCATTCAGCAGCAGCAAAACGAAGC AGTCTGGGTCTCTGAAGGAAAGATGGAAGGGATGGATGATGATGCTGAAGACCGCATGTCCTACTTAACC GCCATGGGCGCGGACTATCTGAGTTGTGACAGCCGTCTCATCAGTGACTTTGAAGACACCGACGGCGAGG GAGGCGCCTACACTGACAATGAGCTAGAGGAGCCGGCGGAGGAGCCGCTGGTGTCTTCCATCACCCGCTC CTCGGAGCCGGTGCAGCACGAGGAGAACATAAGGAAATCCAGCCCAGAGCCACGAGCTCAGATGAGGAGG GCTGCTAGCAGAGACCAACTTAGGGATGCTAGCCCGCCCCCAGCCTTCAAGCCAGAGCCGCCCAAGGCAA GAAGCCAGAACAGAGAAGACTCCTTCGACTACTCCAAGTCAAACCTCCCCGCCACAGCCGGCAGTGAAAT CCCGGGGGGATCCACCAAAGGCTATCCTCCCCCTATTGCAGCGAAGCCTGCCTTTGGGCGGCCCATCCTG AAGCCTTCCACTCCAGTCCCTATGCCTGAGAGCGAGGAGGTTGGAGAGAGCACCGAGGAGCAGGAAGATG CTCCCAGATCAGTCCTGGGCAGAGTGAAAATATTCGAGAAGATGGACCACAAGGCAAAATTACAGAGGAT GCAGGAGCTCCAGGAGGCCCAGAATGCGAGGATCGAAATAGCTCAGAAGCACCCTGACATCTATGCGGTT CCAATCAAGGCCCCCAAGCCAGATGCTGGCCTCCCCCCGCACATGAGTTCTAGACCCCCAGAGCCCCAGA AAGCTCCTTCCAGACTTTACCAGGACACCAGAGGAAGCTACGGCAGTGACCCCGAGGAGGAGGAGTACCG CCAGCAGTTGGCAGCACACTCGAAGCGTGGTTACTACAGCCAGCCCTCCCGGTACCGAGACACGGAGTTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231668 representing NM_001198985 Red=Cloning site Green=Tags(s) MPVRGDRGFPPRRELSGWQRGPGMEEVIWEQYTVTLQKDSKRGFGIAVSGGRDNPHFENGETSIVISDVL PGGPADGLLQENDRVVMVNGTPMEDVLHSFAVQQLRKSGKIAAIVVKRPRKVQVAPLQGSPPLSHDDRGF EVIEEFDGRSFRSGYSERSRHSSHDMLSHSWEGNRERGRPHQRTQSRERERSRGRSLERGLDQEDYGRSR ERSRGRSLERGLDRDFVSRDHSRGRSIDRDYDRDYERSYHEAYEPDYGGGYSPSYDRRAHPETRYERSRS REHLRSRSPSPESRSRHEHKGQHDPDRPIGVLLTKSKANEEYGLRLGSQIFIKEMTRTGLATKDGNLHEG DIILKINGTVTENMSLTDARKLIEKSRGKLQLVVLRDSKQTLINIPALNDSDSEVEDISEIESNRSFSPE ERRQQYSDQDYHSSTEKLKERPSSREETSGRLSRMGATPTPFKSTGDITAAGVTEASREPRYQEEGPVPQ PRTAPRVFLRPSPEDEAIYGPNTKMVRFKKGDSVGLRLAGGNDVGIFVAGIQEGTSAEQEGLQEGDQILK VNTQDFRGLVREDAVLYLLEIPKGETVTILAQSRADVYRDILACGRGDSFFIRSHFECEKETPQSLAFTR GEVFRVVDTLYDGKLGHWLAVRIGNELEKGLIPNKSRAEQMASVQNAQRENAGDRADFWRMRGQRSSGGV KKNLRKSREDLAAAVSVSTKFPAYEKVLLREAGFKRPVVLFGPIADIAMERLATELPDLFQTAKTEPKDA GSEKSSGVVRLNTVRQIIEQDKHALLDVTPKAVDLLNYTQWFPIVIFFNPDSRQGVKTIRQRLSPTSNKS SRKLFDQANKLKKTCSHLFTATINVNSANDGWFGSLKDSIQQQQNEAVWVSEGKMEGMDDDAEDRMSYLT AMGADYLSCDSRLISDFEDTDGEGGAYTDNELEEPAEEPLVSSITRSSEPVQHEENIRKSSPEPRAQMRR AASRDQLRDASPPPAFKPEPPKARSQNREDSFDYSKSNLPATAGSEIPGGSTKGYPPPIAAKPAFGRPIL KPSTPVPMPESEEVGESTEEQEDAPRSVLGRVKIFEKMDHKAKLQRMQELQEAQNARIEIAQKHPDIYAV PIKAPKPDAGLPPHMSSRPPEPQKAPSRLYQDTRGSYGSDPEEEEYRQQLAAHSKRGYYSQPSRYRDTEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198985
ORF Size	3570 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198985.2
RefSeq Size	4637 bp
RefSeq ORF	3573 bp
Locus ID	21873
MW	134.3 kDa
Gene Summary	Plays a role in tight junctions and adherens junctions.[UniProtKB/Swiss-Prot Function]

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