Pan2 (NM_001252326) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	1200014O24Rik; AI047843; AW742773; mKIAA0710; Usp52
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229459 representing NM_001252326 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTTTGAGGGTCTGGATCCTGGACTGGCCGAATTTTCCCCAGCTATGCATTCTACCCTGGACCCAG TCCTGGATGCCCACCTGAATCCAAGTTTGCTACAGAATGTGGAGCTAGACCCAGAGGGAGTGGCCTTGGA GGCTCTTCCTGTCCAGGAGTCAGTGCACATAATGGAAGGTGTCTACTCTGAGTTGCACAGCGTGGTGGCT GAAGTGGGAGTGCCTGTGTCTGTCTCCCACTTTGATTTACATGAGGAGATGCTGTGGGTGGGGAGCCATG GGGGCCATGCCACCTCCTTCTTCGGCCCAGCTCTGGAGCGCTACTCATCCTTTCAGGTCAATGGTGGTGA CGACATTCGGCAAATCCAGAGCCTGGAGAACGGTATCCTCTTCCTCACCAAGAACAACCTCAAGTACATG GCCCGTGGAGGGCTCATTATATTTGACTATTTGCTGGATGAAAACGAGGATATGCACAGCGTCCTCCTGA CAGACAACAGCACTCTGCTCGTTGGGGGGCTGCAGAACCACGTACTGGAGATTGACCTGAACACTGTCCA GGAGACTCAGAAGTATGCAGTCGAGACACCCGGAGTCACCATCATGAGACAGACAAATCGTTTCTTCTTC TGTGGCCACACATCTGGCAAGGTTTCCCTGCGAGACCTCCGTAGTTTTAAAGTGGAGCATGAATTTGATG CCTTCTCAGGGAGTCTGTCAGATTTTGATGTTCATGGCAACCTGCTGGCTGCCTGCGGCTTCTCCAGTCG CCTTACCGGCCTGGCCTGTGACCGTTTCCTCAAAGTGTACGATCTGCGCATGATGCGTGCCATCACACCT CTTCAAGTGCACGTGGATCCGGCCTTCTTACGATTCATCCCCACATACACTTCCCGCCTTGCTATCATCT CCCAGTCAGGTCAATGCCAGTTTTGTGAACCCACAGGCCTGGCCAACCCAGCCGACATTTTCCATGTGAA TCCCGTGGGACCTTTGCTAATGACGTTTGATGTGTCAGCCAGCAAGCAGGCCCTGGCCTTTGGGGATTCT GAGGGCTGCGTGCATCTCTGGACTGATTCCCCTGAGCCGTCCTTCAACCCCTACTCCCGAGAGACTGAGT TTGCCCTGCCCTGTCTTGTGGACTCCTTGCCGCCTCTGGACTGGAGCCAGGATCTGCTGCCGCTTTCCCT CATTCCCGTCCCACTTACCACTGATGCACTGCTCTCTGACTGGCCTGCTGCCAACTCTGCTCCCGCTCCC AGGCGAGCACCACCTGTGGATGCAGAAATTCTTCGAACCATGAAGAAAGTGGGCTTCATTGGCTATGCTC CCAACCCTCGCACCAGGCTGCGCAACCAGATTCCCTATCGACTAAAGGAGTCAGACCATGAATTTGACAA CTTCAGCCAAGTCACGGAGTCACCGACAGGGCGAGAAGAGGAGCCTCTCCACACAGTTTCTAAGAAATAC CGGAAGGTAACCATCAAATATTCCAAGCTAGGCCTGGAAGACTTTGACTTCAAACACTACAATAAGACTC TGTTTGCTGGGTTAGAGCCTCACATCCCCAATGCCTACTGTAACTGCATGATCCAGGTGCTCTATTTCTT GGAGCCTGTTCGCTGTCTGATCCAGAACCACCTTTGCCAAAAGGAGTTCTGCCTAGCGTGCGAGCTCGGC TTCCTCTTCCACATGCTAGACCTCTCTCGTGGCGATCCTTGTCAGGGCAGTAATTTCCTCCGAGCTTTCC GGACCATCCCTGAGGCCTCAGCTCTGGGTCTCATCCTTGCTGACTCAGATGAGGCTTCAGGCAAGGGCAG TCTGGCCCGGCTCATCCAGAGGTGGAACCGCTTCATCCTCACTCAGCTACATCAGGATATGCAGGAGCTG GAAGTGCCCCAGGCTTATCGAGGTGCTGGAGGCAGTTTCTGCTCATCAGGAGACTCCATCATTGGGCAGC TGTTCAGTTGTGAGATGGAGAACTGCAGTCTTTGCCGCTGCGGCAGCGAGACTGTGCGGGCCTCGTCCAC CCTACTCTTCACACTCTCCTACCCTGAGGATAAAACCGGGAAGAACTATGACTTTGCTCAGGTGCTGAAG CGAAGTATCTGCCTGGAGCAGAACACACAGGCCTGGTGTGACAACTGTGAGAAGTACCAGCCCACAATTC AGACCAGAAACATCCGACATCTGCCAGATATTCTTGTCATTAACTGTGAAGTGAATAGCTCAAAAGAGGC TGATTTCTGGAGACTGCAAGCTGAGGTTGCCTTCAAAATAGCGGTGAAAAAGTACGGCGGGGAAATGAAG AGCAAAGAGTTTGCTTTAGCTGATCGGAAGGAACTGAGGAGTCCAGAGGGCTTTCTGTGTTCCTCCATCG AGGAGCTGAAGAATGTCTGGCTTCCATTTTCCATCCGCATGAAGATGACCAAGAACAAAGGACTGGATGT TTGCAACTGGGCTGACGAGCATGAGTGGGGTCCGGCCAGGGCAGAGGAGGAGCTTGGTGTCTATGTATAT GACCTGATGGCTACTGTGGTACACATCCTGGACTCACGAACAGGCGGCAGCTTGGTGGCTCACATCAAAG TCGGTGAGACCTACCACCAGCGCAAGGAGGGCGTGACTCATCAGCAGTGGTATCTCTTCAATGACTTCCT TATTGAACCTATTGATAAGTATGAAGCCGTGCAGTTTGACATGAATTGGAAAGTACCTGCTATCCTGTAT TATGTCAAAAGGAATCTCAATTCCAGATACAATCTGAATATCAAGAACCCCATTGAGGCCAGTGTGCTGC TGGCAGAAGCCTCGCTGGCACGGAAGCAACGGAAAACACACACGACCTTTATTCCATTGATGCTGAATGA GATGCCACAGGTCGGGGACCTGGTGGGCCTGGATGCCGAGTTTGTCACTCTTAATGAGGAAGAAGCAGAG TTACGCAGTGATGGTACCAAGTCCACCATTAAGCCAAGCCAGATGTCAGTAGCAAGGATCACCTGTGTTC GGGGCCAAGGGCCTAACGAGGGCATCCCCTTCATTGATGACTACATCTCCACGCAGGAGCAGGTAGTAGA TTACTTGACTCAGTACTCGGGGATAAAGCCAGGAGACCTTGATGCCAAAATCTCCTCAAAGCACCTCACA ACCCTCAAGTCTACCTACTTAAAGCTTCGCTTTCTCATTGATATTGGAGTCAAGTTTGTGGGTCATGGTC TGCAGAAGGACTTCCGGGTCATCAACCTCATGGTGCCCAAGGACCAAGTTCTTGACACAGTCTACCTGTT CCACATGCCCCGAAAACGAATGATTTCCCTACGATTCCTGGCCTGGTATTTTCTAGACCTGAAGATTCAA GGTGAGACCCATGACAGCATTGAGGATGCCCGCACAGCCCTTCAACTCTACCGGAAGTATCTGGAGCTGA GCAAGAACGGCACCGAGCCCGAGTCCTTCCACAAGGTGCTCAAGGGTCTCTATGAGAAGGGCCGAAAGAT GGACTGGAAGGTGCCTGAGCCTGAAAGCCAGACAAGTCCCAAGAATGCAGCTGTCTTCTCAGTGCTGGCA CTGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001252326
Insert Size	3576 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001252326.1, NP_001239255.1
RefSeq Size	4423 bp
RefSeq ORF	3576 bp
Locus ID	103135
UniProt ID	Q8BGF7
Gene Summary	Catalytic subunit of the poly(A)-nuclease (PAN) deadenylation complex, one of two cytoplasmic mRNA deadenylases involved in general and miRNA-mediated mRNA turnover. PAN specifically shortens poly(A) tails of RNA and the activity is stimulated by poly(A)-binding protein (PABP). PAN deadenylation is followed by rapid degradation of the shortened mRNA tails by the CCR4-NOT complex. Deadenylated mRNAs are then degraded by two alternative mechanisms, namely exosome-mediated 3'-5' exonucleolytic degradation, or deadenlyation-dependent mRNA decaping and subsequent 5'-3' exonucleolytic degradation by XRN1 (PubMed:16284618). Also acts as an important regulator of the HIF1A-mediated hypoxic response. Required for HIF1A mRNA stability independent of poly(A) tail length regulation (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (2) uses a different splice site in the coding region, compared to variant 1. The resulting protein (isoform 2) is shorter when it is compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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