Adgrb2 (NM_001290714) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Bai2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229601 representing NM_001290714 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGATTCTGTCCCTGCGCCTGGCCACGGCCTTCGACCCTG CCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGCGTGCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCC CACCATCGCCTCGGGCTGCTCCTGGACCCTGGAGAACCCAGACCCCACCAAGTACTCCCTCTACCTGCGC TTCAACCGGCAGGAGCAGGTTTGCACACACTTTGCCCCGCGCCTGCTGCCCCTGGACCACTACCTGGTCA ACTTTACCTGCCTGCGGCCTGGTCCAGAGGAAGCCACAGCCCGGGCTGAGTCGGAGGTGGGACGGCCAGA GGAGGAGGAGGAGGAGGCGGCGGCAGCAGCATCAGGGTTGGAGTTGTGTGGTGGCTCAGGCCCCTTTACC TTTCTGCACTTCGACAAGAACTTTGTGCAGCTGTGCCTGTCGGCTGAGCCCTCTGAGGCCCCTCGTCTGC TAGCGCCTGCTGCCCTGGCCTTCCGTTTTGTGGAGGTCTTGCTGATCAACAACAACAACTCCAGCCAGTT CACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGGGCTGCAGGCAGGGCTTGTGGCTTTGCA CAGCCAGGGTGTAGTTGTCCTGGGGAGGCAGGGGCCAACCCCGCCACCACCACATCTCCGGGGCCTCCGG TTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGCCCCTCCTGCTGAGGCCGACTTGCACTC GGGGAGCAGCAATGACCTGTTCACCACCGAGATGAGATATGGTGAGGAGCCGGAAGAGGAACCGAAGGTG AAAACCCAGTGGCCAAGGTCTGCAGATGAGCCTGGGCTATACATGGCGCAGACAGGCGACCCAGCAGCTG AGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCAGGGTCTGCAGGTGCGGACCCGCTCCTG CGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTTCGGGAGACCCGGCCTTGCAACAATTCAGCC ACCTGCCCAGTGGAAGGCCAGTGGCTAGAATGGGGTCCCTGGGGCCCATGCTCATCATCTTGTGCCAATG GGACCCAGCAGCGCAGCCGGAAATGCAGTGTGGCGGGTCCAGCCTGGGCCACGTGCGCAGGTGCCCTCAC GGATACCCGTGAGTGCAGCAATCTCGATTGCCCGGCCACTGACGGCAAGTGGGGGCCGTGGAACGCGTGG AGCCTGTGCTCCAAGACGTGTGACACGGGCTGGCAACGCCGCTTCCGCATGTGCCAGGCTTCTGGCACAC AGGGCTACCCTTGCGAGGGCACAGGAGAGGAGGTGAAACCCTGCAGTGAGAAGAGGTGTCCAGCCTTCCA TGAGATGTGCAGGGATGAGTACGTGATGTTGATGACATGGAAGAGGGCGGCAGCTGGCGAGATCATTTAC AACAAGTGTCCCCCTAATGCCTCGGGTTCTGCTAGCCGCCGCTGTCTCCTCAGTGCCCAGGGCGTAGCAT ACTGGGGACTGCCCAGCTTTGCTCGTTGCATATCCCATGAATACCGCTACCTGTACCTGTCACTTCGGGA ACACCTGGCTAAGGGCCAGCGCATGCTGGCAGGTGAGGGCATGTCACAGGTGGTGCGGAGCCTGCAGGAG CTACTGGCACGGCGCACTTACTACAGCGGGGACCTGCTCTTCTCTGTGGACATCCTAAGGAACGTCACTG ACACCTTCAAGAGGGCCACCTATGTCCCTTCCGCCGATGACGTGCAGCGTTTCTTCCAGGTGGTGAGCTT CATGGTGGATTCAGAAAACAAGGACAAATGGGATGATGCTCAGCAGGTGTCACCGGGCTCTGTGCACCTG CTGCGTGTTGTGGAAGATTTCATTCACCTCGTGGGCGACGCTCTCAAGGCCTTCCAGAGCTCTCTCATTG TCACGGACAATCTGGTGATCAGCATTCAGAGAGAGCCTATCTCCGCCGTGTCCAGTGACATCACGTTTCC CATGCGGGGCCGCAGGGGCATGAAGGACTGGGTGCGACACTCAGAGGATCGTCTCTTTCTACCCAAGGAG GTGCTCAGCCTGTCCTCCCCAGGAAAGCCAGCCACACCTGGGGCAGCCACAGCAGGCAGCCCGGGCAGGG GGAGGGGCCCAGGAACGGTGCCCCCTGGCCCAGGCCACGCCCACCAGCGCCTTCTCCCAGCTGACCCCGA AGAGTCCTCCTCCTACTTTGTGATCGGTGCTGTGCTCTACCGCACCCTTGGCCTCATCCTGCCGCCCCCC AGGCCTCCACTTGCTGTCACCTCCCGGGTGATGACAGTGACTGTGCGTCCCCCCACCCAGCCTCCAGCTG AGCCCCTCATTACAGTGGAACTCTCGTACATCATCAATGGCACCACCGATCCCCACTGTGCCAGCTGGGA CTACTCCAGAGCAGATACCAACTCGGGGGACTGGAACACTGAGAGCTGCCAGACCTTGGAGACCCAGGCG GCTCACACCCGCTGCCAGTGCCAGCACCTGTCCACCTTTGCCGTCCTGGCCCAGCCACCCAAGGACCTGA CCCTGGAGCTGGCAGGTGCTCCCTCTGTCCCCCTGGTGATCGGCTGTGCAGTGTCCTGCATGGCTCTGCT CACCCTGCTGGCCATCTATGCAGCCTTCTGGAGGTTTATAAAATCAGAACGCTCCATCATCTTGCTGAAC TTCTGCCTGTCCATCCTGGCTTCCAACATTCTGATCCTGGTGGGCCAGTCCCGGGTGCTGAGCAAGGGCG TATGCACCATGACGGCTGCCTTCCTACACTTCTTCTTTCTGTCCTCCTTTTGCTGGGTGCTTACAGAGGC CTGGCAATCCTATCTGGCTGTCATCGGGCGGATGCGCACCCGCCTGGTTCGCAAGCGCTTCCTCTGCCTG GGCTGGGGTCTACCTGCCCTGGTGGTGGCTGTGTCTGTTGGCTTTACTCGCACCAAAGGATATGGTACAT CCAGCTACTGCTGGCTGTCCCTAGAGGGCGGCCTGCTCTATGCCTTCGTGGGTCCAGCAGCAGTCATTGT CCTGGTGAACATGCTCATCGGGATTATCGTCTTCAACAAGCTCATGGCTCGCGATGGCGTCTCAGACAAA TCTAAGAAGCAGAGGGCTGGGGCTTCACTCTGGAGTTCCTGCGTGGTACTGCCTCTCCTGGCGCTTACCT GGATGTCTGCCGTCCTGGCCATGACAGATCGCCGCTCCGTCCTCTTCCAGGCACTCTTTGCCGTTTTCAA CTCTGCACAAGGCTTTGTCATCACCGCTGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTAAAG TGTCAGATGGGTGTGTGTCGGGCTGATGAGAGTGAAGACTCCCCAGACTCGTGCAAGAACGGGCAGCTGC AGATCCTGTCAGACTTTGAAAAGGACGTGGATCTGGCTTGTCAGACAGTTCTGTTCAAGGAGGTCAACAC CTGCAACCCGTCCACCATTACCGGCACTCTGTCCCGCCTGTCTCTGGATGAGGATGAGGAGCCCAAGTCC TGTCTCGTGGGTCCTGAGGGTGGCCTCAGCTTCTCACCACTGCCTGGGAACATCCTGGTACCCATGGCAG CCTCACCAGGTCTAGGGGAGCCACCACCACCCCAGGAGACCAACCCTGTGTACATGTGTGGGGAGGGTGG CCTGCGGCAGTTGGACCTTACATGGATACGGCAGAGTGAACCAGGCTCTGAGGGGGACTACATGGTTCTG CCCCGGCGGACTTTGAGCCTGCAGCCTGGTGGTGGGGGTACAGCGGGTGAGGAGGCCCCAAGGGCCCGGC CTGAGGGGACCCCCCGGCGGGCTGCCAAAACGGTAGCCCACACTGAAGGCTACCCCAGCTTCCTGTCTGT GGAGCACTCGGGTCTAGGGCTGGGCCCTGCCTATGGGTCTCTCCAGAACCCGTATGGAATGACCTTCCAA CCACCACCACCAACACCCAGCGCCCGCCAAGTACCAGAGCCAGGAGAACGTAGCCGGACCATGCCCCGTA CAGTGCCTGGTTCCACCATGAAGCTGGGCTCCCTTGAGCGAAAGAAGCTTCGGTATTCGGACTTGGACTT TGAGAAGGTGATGCACACTCGGAAACGGCACTCGGAACTCTACCACGAACTCAACCAGAAGTTCCACACT TTCGACCGCTACCGTAGCCAGTCCTCAGCCAAGGAGAAACCCAGCCCCCCCGGGGGACGCCCTGGCTTGT CCCAGCACAGGAGGCATCAAAGCTGGAGCACCTTCAAATCTATGACACTGGGCTCACTGCCCCCCAAGCC CCGAGAACGGCTGGCCCTGCACCGGACAGCAGCCTGGGAGCCCACAGAACCGCCAGACGGCGACTTCCAG ACAGAGGTGTGA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-RsrII
ACCN	NM_001290714
Insert Size	4422 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001290714.1, NP_001277643.1
RefSeq Size	5185 bp
RefSeq ORF	4422 bp
Locus ID	230775
UniProt ID	Q8CGM1
Gene Summary	Orphan G-protein coupled receptor involved in cell adhesion and probably in cell-cell interactions. Activates NFAT-signaling pathway, a transcription factor, via the G-protein GNAZ. Involved in angiogenesis inhibition (PubMed:12218411).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks one alternate in-frame exon each, in the 5' and 3' coding regions, compared to variant 1. It encodes isoform 4, which is shorter than isoform 1.

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