Adgrb2 (NM_001290714) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Bai2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231812 representing NM_001290714 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCCAGCCTGTCCCCTCTTACTGTCTGTGATTCTGTCCCTGCGCCTGGCCACGGCCTTCGACCCTG CCCCCAGTGCCTGCTCTGCCCTGGCCTCGGGCGTGCTCTACGGGGCCTTCTCGCTGCAGGACCTCTTTCC CACCATCGCCTCGGGCTGCTCCTGGACCCTGGAGAACCCAGACCCCACCAAGTACTCCCTCTACCTGCGC TTCAACCGGCAGGAGCAGGTTTGCACACACTTTGCCCCGCGCCTGCTGCCCCTGGACCACTACCTGGTCA ACTTTACCTGCCTGCGGCCTGGTCCAGAGGAAGCCACAGCCCGGGCTGAGTCGGAGGTGGGACGGCCAGA GGAGGAGGAGGAGGAGGCGGCGGCAGCAGCATCAGGGTTGGAGTTGTGTGGTGGCTCAGGCCCCTTTACC TTTCTGCACTTCGACAAGAACTTTGTGCAGCTGTGCCTGTCGGCTGAGCCCTCTGAGGCCCCTCGTCTGC TAGCGCCTGCTGCCCTGGCCTTCCGTTTTGTGGAGGTCTTGCTGATCAACAACAACAACTCCAGCCAGTT CACCTGTGGTGTGCTCTGCCGCTGGAGTGAGGAGTGTGGCCGGGCTGCAGGCAGGGCTTGTGGCTTTGCA CAGCCAGGGTGTAGTTGTCCTGGGGAGGCAGGGGCCAACCCCGCCACCACCACATCTCCGGGGCCTCCGG TTGCCCACACCCTGTCCAATGCCCTGGTGCCCGGGGGCCCAGCCCCTCCTGCTGAGGCCGACTTGCACTC GGGGAGCAGCAATGACCTGTTCACCACCGAGATGAGATATGGTGAGGAGCCGGAAGAGGAACCGAAGGTG AAAACCCAGTGGCCAAGGTCTGCAGATGAGCCTGGGCTATACATGGCGCAGACAGGCGACCCAGCAGCTG AGGAGTGGTCCCCGTGGAGCGTGTGTTCCCTGACGTGTGGGCAGGGTCTGCAGGTGCGGACCCGCTCCTG CGTGTCCTCCCCCTATGGGACCCTGTGCAGCGGGCCCCTTCGGGAGACCCGGCCTTGCAACAATTCAGCC ACCTGCCCAGTGGAAGGCCAGTGGCTAGAATGGGGTCCCTGGGGCCCATGCTCATCATCTTGTGCCAATG GGACCCAGCAGCGCAGCCGGAAATGCAGTGTGGCGGGTCCAGCCTGGGCCACGTGCGCAGGTGCCCTCAC GGATACCCGTGAGTGCAGCAATCTCGATTGCCCGGCCACTGACGGCAAGTGGGGGCCGTGGAACGCGTGG AGCCTGTGCTCCAAGACGTGTGACACGGGCTGGCAACGCCGCTTCCGCATGTGCCAGGCTTCTGGCACAC AGGGCTACCCTTGCGAGGGCACAGGAGAGGAGGTGAAACCCTGCAGTGAGAAGAGGTGTCCAGCCTTCCA TGAGATGTGCAGGGATGAGTACGTGATGTTGATGACATGGAAGAGGGCGGCAGCTGGCGAGATCATTTAC AACAAGTGTCCCCCTAATGCCTCGGGTTCTGCTAGCCGCCGCTGTCTCCTCAGTGCCCAGGGCGTAGCAT ACTGGGGACTGCCCAGCTTTGCTCGTTGCATATCCCATGAATACCGCTACCTGTACCTGTCACTTCGGGA ACACCTGGCTAAGGGCCAGCGCATGCTGGCAGGTGAGGGCATGTCACAGGTGGTGCGGAGCCTGCAGGAG CTACTGGCACGGCGCACTTACTACAGCGGGGACCTGCTCTTCTCTGTGGACATCCTAAGGAACGTCACTG ACACCTTCAAGAGGGCCACCTATGTCCCTTCCGCCGATGACGTGCAGCGTTTCTTCCAGGTGGTGAGCTT CATGGTGGATTCAGAAAACAAGGACAAATGGGATGATGCTCAGCAGGTGTCACCGGGCTCTGTGCACCTG CTGCGTGTTGTGGAAGATTTCATTCACCTCGTGGGCGACGCTCTCAAGGCCTTCCAGAGCTCTCTCATTG TCACGGACAATCTGGTGATCAGCATTCAGAGAGAGCCTATCTCCGCCGTGTCCAGTGACATCACGTTTCC CATGCGGGGCCGCAGGGGCATGAAGGACTGGGTGCGACACTCAGAGGATCGTCTCTTTCTACCCAAGGAG GTGCTCAGCCTGTCCTCCCCAGGAAAGCCAGCCACACCTGGGGCAGCCACAGCAGGCAGCCCGGGCAGGG GGAGGGGCCCAGGAACGGTGCCCCCTGGCCCAGGCCACGCCCACCAGCGCCTTCTCCCAGCTGACCCCGA AGAGTCCTCCTCCTACTTTGTGATCGGTGCTGTGCTCTACCGCACCCTTGGCCTCATCCTGCCGCCCCCC AGGCCTCCACTTGCTGTCACCTCCCGGGTGATGACAGTGACTGTGCGTCCCCCCACCCAGCCTCCAGCTG AGCCCCTCATTACAGTGGAACTCTCGTACATCATCAATGGCACCACCGATCCCCACTGTGCCAGCTGGGA CTACTCCAGAGCAGATACCAACTCGGGGGACTGGAACACTGAGAGCTGCCAGACCTTGGAGACCCAGGCG GCTCACACCCGCTGCCAGTGCCAGCACCTGTCCACCTTTGCCGTCCTGGCCCAGCCACCCAAGGACCTGA CCCTGGAGCTGGCAGGTGCTCCCTCTGTCCCCCTGGTGATCGGCTGTGCAGTGTCCTGCATGGCTCTGCT CACCCTGCTGGCCATCTATGCAGCCTTCTGGAGGTTTATAAAATCAGAACGCTCCATCATCTTGCTGAAC TTCTGCCTGTCCATCCTGGCTTCCAACATTCTGATCCTGGTGGGCCAGTCCCGGGTGCTGAGCAAGGGCG TATGCACCATGACGGCTGCCTTCCTACACTTCTTCTTTCTGTCCTCCTTTTGCTGGGTGCTTACAGAGGC CTGGCAATCCTATCTGGCTGTCATCGGGCGGATGCGCACCCGCCTGGTTCGCAAGCGCTTCCTCTGCCTG GGCTGGGGTCTACCTGCCCTGGTGGTGGCTGTGTCTGTTGGCTTTACTCGCACCAAAGGATATGGTACAT CCAGCTACTGCTGGCTGTCCCTAGAGGGCGGCCTGCTCTATGCCTTCGTGGGTCCAGCAGCAGTCATTGT CCTGGTGAACATGCTCATCGGGATTATCGTCTTCAACAAGCTCATGGCTCGCGATGGCGTCTCAGACAAA TCTAAGAAGCAGAGGGCTGGGGCTTCACTCTGGAGTTCCTGCGTGGTACTGCCTCTCCTGGCGCTTACCT GGATGTCTGCCGTCCTGGCCATGACAGATCGCCGCTCCGTCCTCTTCCAGGCACTCTTTGCCGTTTTCAA CTCTGCACAAGGCTTTGTCATCACCGCTGTGCACTGCTTCCTGCGCCGAGAGGTCCAGGATGTGGTAAAG TGTCAGATGGGTGTGTGTCGGGCTGATGAGAGTGAAGACTCCCCAGACTCGTGCAAGAACGGGCAGCTGC AGATCCTGTCAGACTTTGAAAAGGACGTGGATCTGGCTTGTCAGACAGTTCTGTTCAAGGAGGTCAACAC CTGCAACCCGTCCACCATTACCGGCACTCTGTCCCGCCTGTCTCTGGATGAGGATGAGGAGCCCAAGTCC TGTCTCGTGGGTCCTGAGGGTGGCCTCAGCTTCTCACCACTGCCTGGGAACATCCTGGTACCCATGGCAG CCTCACCAGGTCTAGGGGAGCCACCACCACCCCAGGAGACCAACCCTGTGTACATGTGTGGGGAGGGTGG CCTGCGGCAGTTGGACCTTACATGGATACGGCAGAGTGAACCAGGCTCTGAGGGGGACTACATGGTTCTG CCCCGGCGGACTTTGAGCCTGCAGCCTGGTGGTGGGGGTACAGCGGGTGAGGAGGCCCCAAGGGCCCGGC CTGAGGGGACCCCCCGGCGGGCTGCCAAAACGGTAGCCCACACTGAAGGCTACCCCAGCTTCCTGTCTGT GGAGCACTCGGGTCTAGGGCTGGGCCCTGCCTATGGGTCTCTCCAGAACCCGTATGGAATGACCTTCCAA CCACCACCACCAACACCCAGCGCCCGCCAAGTACCAGAGCCAGGAGAACGTAGCCGGACCATGCCCCGTA CAGTGCCTGGTTCCACCATGAAGCTGGGCTCCCTTGAGCGAAAGAAGCTTCGGTATTCGGACTTGGACTT TGAGAAGGTGATGCACACTCGGAAACGGCACTCGGAACTCTACCACGAACTCAACCAGAAGTTCCACACT TTCGACCGCTACCGTAGCCAGTCCTCAGCCAAGGAGAAACCCAGCCCCCCCGGGGGACGCCCTGGCTTGT CCCAGCACAGGAGGCATCAAAGCTGGAGCACCTTCAAATCTATGACACTGGGCTCACTGCCCCCCAAGCC CCGAGAACGGCTGGCCCTGCACCGGACAGCAGCCTGGGAGCCCACAGAACCGCCAGACGGCGACTTCCAG ACAGAGGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231812 representing NM_001290714 Red=Cloning site Green=Tags(s) MTPACPLLLSVILSLRLATAFDPAPSACSALASGVLYGAFSLQDLFPTIASGCSWTLENPDPTKYSLYLR FNRQEQVCTHFAPRLLPLDHYLVNFTCLRPGPEEATARAESEVGRPEEEEEEAAAAASGLELCGGSGPFT FLHFDKNFVQLCLSAEPSEAPRLLAPAALAFRFVEVLLINNNNSSQFTCGVLCRWSEECGRAAGRACGFA QPGCSCPGEAGANPATTTSPGPPVAHTLSNALVPGGPAPPAEADLHSGSSNDLFTTEMRYGEEPEEEPKV KTQWPRSADEPGLYMAQTGDPAAEEWSPWSVCSLTCGQGLQVRTRSCVSSPYGTLCSGPLRETRPCNNSA TCPVEGQWLEWGPWGPCSSSCANGTQQRSRKCSVAGPAWATCAGALTDTRECSNLDCPATDGKWGPWNAW SLCSKTCDTGWQRRFRMCQASGTQGYPCEGTGEEVKPCSEKRCPAFHEMCRDEYVMLMTWKRAAAGEIIY NKCPPNASGSASRRCLLSAQGVAYWGLPSFARCISHEYRYLYLSLREHLAKGQRMLAGEGMSQVVRSLQE LLARRTYYSGDLLFSVDILRNVTDTFKRATYVPSADDVQRFFQVVSFMVDSENKDKWDDAQQVSPGSVHL LRVVEDFIHLVGDALKAFQSSLIVTDNLVISIQREPISAVSSDITFPMRGRRGMKDWVRHSEDRLFLPKE VLSLSSPGKPATPGAATAGSPGRGRGPGTVPPGPGHAHQRLLPADPEESSSYFVIGAVLYRTLGLILPPP RPPLAVTSRVMTVTVRPPTQPPAEPLITVELSYIINGTTDPHCASWDYSRADTNSGDWNTESCQTLETQA AHTRCQCQHLSTFAVLAQPPKDLTLELAGAPSVPLVIGCAVSCMALLTLLAIYAAFWRFIKSERSIILLN FCLSILASNILILVGQSRVLSKGVCTMTAAFLHFFFLSSFCWVLTEAWQSYLAVIGRMRTRLVRKRFLCL GWGLPALVVAVSVGFTRTKGYGTSSYCWLSLEGGLLYAFVGPAAVIVLVNMLIGIIVFNKLMARDGVSDK SKKQRAGASLWSSCVVLPLLALTWMSAVLAMTDRRSVLFQALFAVFNSAQGFVITAVHCFLRREVQDVVK CQMGVCRADESEDSPDSCKNGQLQILSDFEKDVDLACQTVLFKEVNTCNPSTITGTLSRLSLDEDEEPKS CLVGPEGGLSFSPLPGNILVPMAASPGLGEPPPPQETNPVYMCGEGGLRQLDLTWIRQSEPGSEGDYMVL PRRTLSLQPGGGGTAGEEAPRARPEGTPRRAAKTVAHTEGYPSFLSVEHSGLGLGPAYGSLQNPYGMTFQ PPPPTPSARQVPEPGERSRTMPRTVPGSTMKLGSLERKKLRYSDLDFEKVMHTRKRHSELYHELNQKFHT FDRYRSQSSAKEKPSPPGGRPGLSQHRRHQSWSTFKSMTLGSLPPKPRERLALHRTAAWEPTEPPDGDFQ TEV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-RsrII      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290714
ORF Size	4419 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290714.2
RefSeq Size	5185 bp
RefSeq ORF	4422 bp
Locus ID	230775
UniProt ID	Q8CGM1
MW	161 kDa
Gene Summary	Orphan G-protein coupled receptor involved in cell adhesion and probably in cell-cell interactions. Activates NFAT-signaling pathway, a transcription factor, via the G-protein GNAZ. Involved in angiogenesis inhibition (PubMed:12218411).[UniProtKB/Swiss-Prot Function]

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