Myo18a (NM_001291214) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	MAJN; MyoPDZ; MysPDZ; SP-R210
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229652 representing NM_001291214 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGCCAAGACGGAAGAGCAGATTGCCGCTGAAGAGGCCTGGTATGAGACGGAGAAGGTGTGGCTGG TCCATAGAGATGGATTCTCCTTAGCCAGCCAGCTCAAGTCTGAGGAGCTCAGCCTACCCGAGGGGAAGGC GCGTGTGAAGCTGGACCATGACGGAGCCATCCTGGATGTGGATGAAGATGATATAGAGAAGGCTAATGCT CCTTCATGTGACCGTCTAGAAGATCTGGCCTCACTGGTATACCTCAACGAGTCTAGCGTCCTGCATACAC TGCGCCAGCGCTATGGGGCTAGCCTGCTACACACCTATGCTGGCCCCAGCCTGCTTGTCCTTAGCACCCG AGGGGCTCCTGCTGTGTATTCAGAGAAGGTGATGCACATGTTCAAGGGGTGTCGGCGGGAGGACATGGCA CCCCACATCTACGCTGTAGCCCAGACTGCATATAGGGCGATGTTGATGAGTCGTCAGGACCAGTCCATTG TCCTTCTGGGTAGTAGTGGCAGTGGCAAGACTACCAGCTTTCAGCATCTGGTGCAGTATCTGGCTACCAT CGCGGGTACCAGTGGGACCAAGGTGTTCTCAGTGGAGAAGTGGCAGGCTCTGTCCACCCTCCTGGAAGCC TTTGGGAACAGCCCCACCATCATGAATGGCAGTGCCACCCGCTTCTCCCAGATCCTCTCCCTGGACTTTG ACCAAGCTGGCCAGGTGGCTTCGGCCTCCATCCAGACAATGCTCCTGGAGAAGCTGCGTGTGGCCCGGCG CCCAGCCAGTGAGGCTACTTTCAATGTCTTCTACTACTTGCTGGCCTGTGGGGATGCCACCCTCAGGACA GAGCTCCACCTGAACCACTTGGCAGAGAACAATGTGTTTGGAATTGTGCCACTGTCCAAGCCTGAGGAGA AGCAGAAGGCTGCACAGCAGTTCAGTAAGCTCCAGGCTGCCATGAAGGTGCTGGCCATCTCCCCCGAGGA ACAGAAGACCTGCTGGCTCATCCTGGCTTCCATCTACCACCTGGGGGCTGCTGGAGCTACCAAAGCTGGA CGAAAGCAGTTTGCCCGCCACGAATGGGCCCAGAAGGCAGCATACCTGCTGGGCTGCAGCTTGGAAGAGC TGTCCTCGGCTATCTTCAAACACCAGCTAAAAGGAGGCACCCTGCAACGGTCCACCTCCTTCCGTCAGGG CCCTGAGGAGAGCGGCCTGGGAGAGGGCACCAAACTGAGTGCTCTGGAGTGCTTAGAGGGTATGGCGTCT GGCCTCTACAGTGAGCTCTTTACTCTCCTCATCTCCCTGGTAAACAGGGCTCTCAAGTCCAGCCAGCACT CACTCTGTTCCATGATGATCGTGGACACACCAGGCTTCCAGAACCCTGAATGGGGCGGCTCGGCCCGTGG AGCCTCCTTCGAGGAGCTGTGCCACAACTATGCCCAGGACAGGCTGCAGAGGCTGTTCCACGAGCGCACC TTCCTCCAGGAGTTGGAGAGATACAAGGAGGATAACATAGAACTGGCGTTTGATGACTTGGAGCCAGTTG CAGATGACTCCGTGGCTGCTGTGGACCAGGCTTCCCACCTGGTTCGCTCACTGGCCCATGCCGATGAGGC AAGAGGGCTACTGTGGCTCCTGGAAGAGGAGGCACTGGTGCCTGGTGCGACAGAGGATGCCCTCCTTGAT CGTCTTTTCTCCTATTATGGCCCCCAGGAGGGTGACAAAAAAGGCCAGAGTCCTCTTCTGCGAAGTAGCA AACCTCGGCACTTTCTCCTGGGCCACAGCCACGGTACCAACTGGGTGGAGTACAACGTGGCTGGCTGGCT GAATTACACCAAGCAGAACCCAGCCACCCAGAATGCACCCCGGCTTCTGCAGGACTCCCAGAAAAAGATC ATCAGTAACCTGTTTCTGGGCCGGGCGGGCAGTGCCACGGTGCTCTCAGGCTCGATTGCAGGCCTGGAAG GTGGCTCCCAGCTGGCCTTGCGCCGGGCAACCAGCATGAGGAAAACCTTCACCACCGGCATGGCCGCTGT CAAGAAGAAGTCGCTGTGCATACAGATTAAGCTGCAAGTAGATGCCCTCATTGATACCATCAAGAGGTCC AAGATGCATTTCGTGCACTGCTTCCTTCCTGTGGCCGAGGGCTGGCCTGGGGAGCCCCGGTCTGCCTCTT CCCGCAGAGTCAGCAGCAGCAGTGAGCTGGACCTGCCCCCGGGAGACCCCTGTGAGGCTGGGCTGCTACA ACTGGATGTGTCCCTGCTCCGAGCCCAGCTCCGGGGATCCCGCCTGCTTGATGCGATGCGCATGTACCGC CAAGGTTACCCAGACCACATGGTGTTTTCTGAGTTCCGCCGGCGCTTTGATGTCCTGGCTCCACACCTGA CCAAGAAACATGGTCGTAACTACATCGTGGTGGATGAGAAGCGGGCTGTGGAAGAGCTGCTGGAGTCCCT GGACCTGGAAAAGAGCAGCTGCTGTCTGGGCCTAAGCCGGGTGTTCTTCCGGGCAGGCACGTTGGCACGG TTGGAAGAACAACGAGACGAGCAAACCAGCAGGCACCTGACCCTGTTCCAGGCAGCCTGCCGGGGCTACC TTGCCCGCCAGCACTTCAAGAAGAGAAAGATCCAGGACCTGGCCATTCGCTGTGTGCAGAAGAACATCAA GAAGAACAAGGGGGTGAAGGACTGGCCCTGGTGGAAGCTCTTCACCACCGTGCGGCCCCTCATCCAAGTT CAGCTGTCAGAGGAGCAGATCCGAAACAAAGACGAGGAGATCCAGCAGCTGCGCAGCAAGCTTGAGAAGG TGGAGAAGGAGAGGAACGAGCTCCGACTCAGCAGTGACCGGCTAGAGACCCGGATCTCAGAACTGACATC GGAGCTGACTGATGAACGCAACACGGGAGAGTCCGCCTCCCAGCTGCTGGACGCGGAGACAGCCGAGAGG CTCCGGACCGAGAAGGAGATGAAAGAGCTACAGACCCAGTATGACGCACTGAAGAAGCAGATGGAGGTGA TGGAGATGGAAGTGATGGAAGCCCGGCTCATTCGGGCAGCCGAGATCAACGGGGAGGTGGATGATGATGA TGCAGGGGGAGAGTGGAGGCTGAAGTACGAGCGAGCTGTTCGGGAGGTGGACTTCACCAAGAAGCGGCTC CAGCAGGAGCTGGAAGACAAGATGGAGGTGGAACAGCAGAGCAGGAGGCAGCTGGAGAGGCGGCTTGGGG ACCTGCAAGCAGATAGTGATGAGAGTCAACGGGCACTGCAGCAGCTCAAGAAGAAGTGCCAAAGGCTCAC AGCTGAGCTGCAGGACACCAAGCTGCACCTGGAGGGCCAGCAGGTCCGAAACCACGAGCTGGAGAAGAAG CAGAGGAGGTTCGACAGTGAGCTTTCCCAGGCCCACGAGGAGACGCAGCGGGAGAAGCTGCAGAGGGAGA AACTCCAGCGGGAGAAGGACATGCTCCTGGCTGAGGCTTTCAGCTTGAAGCAGCAAATGGAGGAAAAAGA CTTGGACATTGCGGGGTTTACCCAGAAGGTGGTTTCCTTGGAGGCTGAGCTTCAGGACATTTCTTCTCAA GAGTCTAAGGATGAGGCTTCTCTGGCCAAGGTCAAGAAGCAGCTCCGGGACTTGGAGGCCAAGGTCAAGG ACCAGGAAGAGGAGCTGGATGAGCAGGCTGGGAGTATACAGATGCTGGAGCAGGCCAAGCTGCGTCTGGA GATGGAGATGGAGAGAATGAGACAGACCCATTCCAAGGAGATGGAGAGTCGGGATGAGGAGGTGGAAGAG GCCCGGCAGTCATGTCAGAAGAAGTTAAAACAGATGGAAGTGCAGCTCGAGGAGGAGTATGAAGACAAGC AGAAGGCGCTGCGGGAGAAACGGGAGCTGGAGAGCAAGCTCTCCACGCTCAGTGACCAGGTGAACCAGCG GGACTTTGAATCAGAGAAGCGGCTACGGAAAGACCTGAAGCGCACCAAAGCGCTGCTGGCAGATGCCCAG ATCATGCTGGACCACTTAAAGAACAATGCCCCGAGCAAGAGGGAGATTGCCCAGCTGAAGAACCAGCTGG AAGAGTCGGAATTCACCTGTGCAGCAGCTGTAAAGGCACGGAAAGCGATGGAGGTGGAGATGGAAGACCT GCACCTGCAGATTGATGACATCGCCAAAGCCAAGACAGCGCTGGAGGAGCAACTGAGTCGACTTCAGCGT GAGAAAAACGAGATTCAAAACCGGCTGGAAGAGGATCAGGAGGACATGAATGAGCTGATGAAGAAGCACA AGGCAGCTGTGGCCCAGGCCTCCCGGGACATGGCACAGATGAACGATCTCCAGGCTCAGATTGAAGAGTC CAACAAGGAGAAGCAAGAGCTACAGGAGAAGCTACAAGCTCTGCAGAGCCAGGTGGAGTTCCTGGAGCAG TCCATGGTGGACAAGTCCCTCGTCAGCAGGCAGGAAGCGAAGATCAGGGAGCTGGAGACACGCCTGGAGT TCGAAAAGACCCAAGTGAAGCGCCTGGAGAACCTGGCCAGTCGGCTCAAAGAAACCATGGAGAAGCTGAC TGAGGAACGGGACCAGCGCGCCGCAGCTGAGAACCGTGAGAAGGAGCAGAACAAGAGGCTCCAGCGACAG CTTCGTGACACCAAGGAGGAGATGAGCGAGCTTGCCAGGAAGGAAGCCGAGGCTAGCCGCAAGAAGCATG AACTGGAGATGGACCTGGAGAGCCTGGAAGCTGCTAACCAAAGCCTGCAAGCCGACCTAAAGCTGGCGTT CAAACGCATTGGGGACTTGCAAGCTGCCATTGAGGATGAGATGGAAAGTGACGAGAACGAGGACCTCATC AACAGTGAGGGGGACTCAGATGTGGACTCAGAGCTGGAGGACCGGGTCGACGGGGTCAAGTCCTGGTTGT CAAAGAACAAGGGACCTTCTAAGGCACCTTCTGACGATGGCAGCTTGAAGAGTTCCAGCCCAACCAGCCA CTGGAAGCCACTCGCCCCTGACCCATCGGATGATGAGCATGATCCTGTGGACAGCATCTCCAGACCCCGG TTCTCCCACAGCTATCTGAGTGACAGCGACACAGAGGCCAAGCTGACAGAGACCAGTGCATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001291214
Insert Size	5103 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001291214.1, NP_001278143.1
RefSeq Size	6351 bp
RefSeq ORF	5103 bp
Locus ID	360013
UniProt ID	Q9JMH9
Gene Summary	May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus (PubMed:19837035). Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration (By similarity). May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (PubMed:10733906). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking (PubMed:25965346). Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages (PubMed:21123169). Strongly enhances natural killer cell cytotoxicity (By similarity).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (4) lacks a portion of the 5' coding region, contains an alternate 5'-terminal exon, uses an alternate start codon, and lacks an exon in the central coding region, compared to variant 1. The encoded isoform (4) is shorter and has a distinct N-terminus, compared to isoform 1.

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