Bloc1s2 (BC065806) Mouse Tagged ORF Clone
CAT#: MG200298
- TrueORF®
Bloc1s2 (tGFP-tagged) - Mouse biogenesis of lysosome-related organelles complex-1, subunit 2 (cDNA clone MGC:73814 IMAGE:1547643)
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CNY 2,850.00
CNY 1,000.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | 2410089B13Rik; Bloc1s2a; BLOS2 |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG200298 representing BC065806
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCTCCAAAATGGCCACATATTTGACTGGGGAACTGACAGCCACCAGTGAAGACTACAAACTCCTGG AAAATATGAATAAGCTAACAAGCTTGAAGTACCTTGAAATGAAAGATATTGCTATCAACATCAGCAGAAA CCTGAAGGACTTAAACCAGAAATATGCTGAACTACAGCCATATCTGGACCAGATCAACATGATTGAGGAG CAGGTGGCAGCTCTGGAGCAGGCAGCCTACAAGCTCGATGCTTATTCAAAAAAACTGGAAGCCAAGTACA AGAAGTTGGAGAAGCGA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG200298 representing BC065806
Red=Cloning site Green=Tags(s) MFSKMATYLTGELTATSEDYKLLENMNKLTSLKYLEMKDIAINISRNLKDLNQKYAELQPYLDQINMIEE QVAALEQAAYKLDAYSKKLEAKYKKLEKR TRTRPLE - GFP Tag - V |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | BC065806 |
ORF Size | 299 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | BC065806, AAH65806 |
RefSeq Size | 962 bp |
RefSeq ORF | 299 bp |
Locus ID | 73689 |
Gene Summary | Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes (By similarity). In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals (PubMed:16760431, PubMed:21998198). The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension (PubMed:19546860). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (By similarity).[UniProtKB/Swiss-Prot Function] |
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