Ibrdc2 (BC025007) Mouse Tagged ORF Clone

CAT#: MG201133

  • TrueORF®

Ibrdc2 (tGFP-tagged) - Mouse IBR domain containing 2 (cDNA clone MGC:32473 IMAGE:5050848)



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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms MGC32473
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG201133 representing BC025007
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGACTGCGGAAAACCCACCCTCTGGAGACCTGATTCCCGCCCCGCTCGTCACTTGCAAGCTCTGCCTGT
GTGAACAGTCTCTGGACAAGATGACCATGCTCCAAGAGTGCCAGTGTATCTTTTGTACACCCTGCCTGAA
ACAGTATATGGTGCTGTCAATCCGAGAAGGCTGTGGATCTCCTATCACTTGTCCTGACATGGTGTGCCTA
AACCACGGGACCCTGCAGGAAACCGAGATTGCCTGTTTGGTGCCTCTGGATGAGTTTCAGCTGTATCAGC
GGCTCAAGTTTGAACGAGAGGTTCACATGGACCCCCTCCGGACATGGTGTCCTGTTGCAGACTGCCAGAC
CGTGTGCCATATTTCAGCCGGAGACCCAGGCCAGCCAGTGTTGGTGGAGTGCCCTTTGCCACCTGAAATT
CTGCTCATGTTGCAAGGATGCTTGGCACGAGGAGTCTTCCTG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG201133 representing BC025007
Red=Cloning site Green=Tags(s)

MTAENPPSGDLIPAPLVTCKLCLCEQSLDKMTMLQECQCIFCTPCLKQYMVLSIREGCGSPITCPDMVCL
NHGTLQETEIACLVPLDEFQLYQRLKFEREVHMDPLRTWCPVADCQTVCHISAGDPGQPVLVECPLPPEI
LLMLQGCLARGVFL

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC025007
ORF Size 464 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC025007, AAH25007
RefSeq Size 2953 bp
RefSeq ORF 464 bp
Locus ID 218215
Gene Summary E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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