Pop5 (NM_026398) Mouse Tagged ORF Clone

CAT#: MG201402

  • TrueORF®

Pop5 (tGFP-tagged) - Mouse processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae) (Pop5)

ORF Plasmid: DDK tGFP



  "NM_026398" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,850.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms 1500019J17Rik; 2700077E03Rik; AW049989; Rnasep3
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG201402 representing NM_026398
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGTGCGGTTCAAGCACAGGTACCTTCTGTGCGAGCTGGTGTCCGAGGACGCGCGCTGCCGTTTGAGCC
TGGACGACCGCGTGCTGGGCGGGCTGGTCCGCGACACGATCGCCCGGGTGCACGGGGCCTTCGGCGCCGC
CGCCTGCTCCGTGGGCTTTGCAGTCCGATACCTCAATGCCTACACTGGAGTCGTGCTACTGCGATGCCGG
AAGGATTTCTACCAGCTCGTGTGGTCAGCTCTTCCTTTCATCACCTATTTGGAGAACAAAGGCCACCGTT
ACCCGTGCTTCTTCAACACATTACACGTGGGAGGTACCATCAGAACCTGTCAGAAGTTCCTGATCCAGTA
CAACAGGAGACAGCTGCTGATCTTGTTGCAGAATTGCACTGACGAAGGAGAGCGGGAAGCCATCAAGAAG
TCTGTCTCAAGAAGCTGTCTGCTGGACAGAGAGCCCGTTGAAGAGCTTTCAGACAGTGCTGGTGAGGAGG
TTGCAGAAGCAATGGAG


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG201402 representing NM_026398
Red=Cloning site Green=Tags(s)

MVRFKHRYLLCELVSEDARCRLSLDDRVLGGLVRDTIARVHGAFGAAACSVGFAVRYLNAYTGVVLLRCR
KDFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRRQLLILLQNCTDEGEREAIKK
SVSRSCLLDREPVEELSDSAGEEVAEAME

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_026398
ORF Size 507 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_026398.4
RefSeq Size 1030 bp
RefSeq ORF 510 bp
Locus ID 117109
UniProt ID Q9DB28
Gene Summary Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. Also a component of the MRP ribonuclease complex, which cleaves pre-rRNA sequences.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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