Tmem97 (BC018156) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	TurboGFP
Synonyms	RP23-399H5.9
Vector	pCMV6-AC-GFP
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MG201535 representing BC018156 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGCCCTGGCAGCTAGGCGCTGTGTCGAGTGGCTCTTGGGCCTCTACTTCGTCTCGCACATCCCCA TCACGCTGTTCATCGACCTGCAGGCGGTGCTGCCGCCCGAACTATACCCGCAGGAGTTCAGCAACCTGTT GCGGTGGTACTCAAAGGAGTTCAAAGACCCTCTGATGCAGGAGCCCCCAGTGTGGTTCAAGTCCTTCCTG CTCTGTGAGCTTGTGTTCCAGCTGCCTTTCTTTCCCATTGCGGCATATGCCTTCTTCAAAGGAAGCTGCC GGTGGATCCGAATCCCTGCAATCATCTATGCAGCTCACACCATAACGACTTTAATTCCAATCCTCTACAC ACTCCTGTTTGAGGATTTCTCTAAAGCCGTTGCTTTCAAGGGACAAAGACCCGAGAGTTTCCGTGAACGA CTGACCCTCGTAGGTGTCTACGCCCCCTATTTAATAATCCCCCTCATACTCCTCCTGTTCATGCTTCGGA ACCCGTACTACAAGTATGAGGAGAAAAGAAAGAAAAAA ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA Protein Sequence (showhide) >MG201535 representing BC018156 Red=Cloning site Green=Tags(s) MGALAARRCVEWLLGLYFVSHIPITLFIDLQAVLPPELYPQEFSNLLRWYSKEFKDPLMQEPPVWFKSFL LCELVFQLPFFPIAAYAFFKGSCRWIRIPAIIYAAHTITTLIPILYTLLFEDFSKAVAFKGQRPESFRER LTLVGVYAPYLIIPLILLLFMLRNPYYKYEEKRKKK TRTRPLE - GFP Tag - V
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	BC018156
ORF Size	530 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	BC018156, AAH18156
RefSeq Size	1347 bp
RefSeq ORF	530 bp
Locus ID	69071
Gene Summary	Intracellular orphan receptor that binds numerous drugs and which is highly expressed in various proliferating cells. Corresponds to the sigma-2 receptor, which is thought to play important role in regulating cell survival, morphology and differentiation. May play a role as a regulator of cellular cholesterol homeostasis. May function as sterol isomerase. May alter the activity of some cytochrome P450 proteins.[UniProtKB/Swiss-Prot Function]

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