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Elovl6 (NM_130450) Mouse Tagged ORF Clone
CAT＃: MG203544
- TrueORF®
Elovl6 (tGFP-tagged) - Mouse ELOVL family member 6, elongation of long chain fatty acids (yeast) (Elovl6)
ORF Plasmid: DDK
"NM_130450" in other vectors (4)
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CNY 2,850.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | TurboGFP |
| Synonyms | C77826; FAE; LCE |
| Vector | pCMV6-AC-GFP |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MG203544 representing NM_130450
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACATGTCAGTGTTGACTTTACAAGAATATGAATTCGAAAAGCAGTTCAACGAGAACGAAGCCATCC AATGGATGCAGGAAAACTGGAAGAAGTCTTTCCTGTTTTCTGCGCTATACGCTGCCTTTATCTTTGGTGG TCGGCATCTGATGAACAAGCGAGCCAAGTTTGAACTTCGGAAGCCGCTCGTGCTCTGGTCGCTGACTCTT GCCGTCTTCAGTATATTCGGTGCTCTTCGAACTGGTGCTTACATGCTGTACATTCTGATGACCAAAGGCC TGAAGCAGTCAGTTTGTGACCAGAGTTTTTACAATGGACCTGTCAGCAAATTCTGGGCTTATGCATTTGT GCTCAGCAAAGCACCCGAACTAGGTGACACGATATTCATCATTCTGAGGAAACAGAAACTGATCTTCCTG CACTGGTACCACCACATCACTGTGCTCCTGTACTCCTGGTACTCCTACAAAGACATGGTCGCTGGGGGTG GTTGGTTCATGACTATGAACTATGGCGTGCATGCCGTCATGTACTCTTACTACGCCTTGCGGGCTGCGGG TTTCCGAGTCTCCCGGAAGTTTGCCATGTTCATCACCTTGTCCCAGATCACTCAGATGCTGATGGGCTGT GTCATTAACTACCTGGTCTTCAACTGGATGCAGCATGACAACGACCAGTGCTACTCCCACTTTCAGAACA TCTTCTGGTCCTCGCTCATGTACCTCAGCTACCTTGTGCTCTTCTGCCATTTCTTCTTTGAGGCCTACAT CGGCAAAGTGAAGAAAGCCACGAAGGCTGAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203544 representing NM_130450
Red=Cloning site Green=Tags(s) MNMSVLTLQEYEFEKQFNENEAIQWMQENWKKSFLFSALYAAFIFGGRHLMNKRAKFELRKPLVLWSLTL AVFSIFGALRTGAYMLYILMTKGLKQSVCDQSFYNGPVSKFWAYAFVLSKAPELGDTIFIILRKQKLIFL HWYHHITVLLYSWYSYKDMVAGGGWFMTMNYGVHAVMYSYYALRAAGFRVSRKFAMFITLSQITQMLMGC VINYLVFNWMQHDNDQCYSHFQNIFWSSLMYLSYLVLFCHFFFEAYIGKVKKATKAE TRTRPLE - GFP Tag - V |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130450 |
| ORF Size | 801 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130450.2, NP_569717.1 |
| RefSeq Size | 5951 bp |
| RefSeq ORF | 804 bp |
| Locus ID | 170439 |
| UniProt ID | Q920L5 |
| Gene Summary | Catalyzes the first and rate-limiting reaction of the four reactions that constitute the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process allows the addition of 2 carbons to the chain of long- and very long-chain fatty acids (VLCFAs) per cycle. Condensing enzyme that elongates fatty acids with 12, 14 and 16 carbons with higher activity toward C16:0 acyl-CoAs. Catalyzes the synthesis of unsaturated C16 long chain fatty acids and, to a lesser extent, C18:0 and those with low desaturation degree. May participate in the production of saturated and monounsaturated VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212315 | Elovl6 (untagged) - Mouse ELOVL family member 6, elongation of long chain fatty acids (yeast) (Elovl6), (10ug) |
CNY 3,990.00 |
|
| MR203544 | Elovl6 (Myc-DDK-tagged) - Mouse ELOVL family member 6, elongation of long chain fatty acids (yeast) (Elovl6) |
CNY 2,400.00 |
|
| MR203544L3 | Lenti ORF clone of Elovl6 (Myc-DDK-tagged) - Mouse ELOVL family member 6, elongation of long chain fatty acids (yeast) (Elovl6) |
CNY 4,750.00 |
|
| MR203544L4 | Lenti ORF clone of Elovl6 (mGFP-tagged) - Mouse ELOVL family member 6, elongation of long chain fatty acids (yeast) (Elovl6) |
CNY 4,750.00 |
