Dnajc27 (NM_153082) Mouse Tagged ORF Clone
CAT#: MG203647
- TrueORF®
Dnajc27 (tGFP-tagged) - Mouse RIKEN cDNA C330021A05 gene (C330021A05Rik)
ORF Plasmid: DDK
"NM_153082" in other vectors (4)
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CNY 2,850.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | TurboGFP |
Synonyms | AI639580; C330021A05Rik; Rabj; Rbj |
Vector | pCMV6-AC-GFP |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MG203647 representing NM_153082
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAACGTGCCGAAGAGGAAGGAGCCCGCCAAGTCCCTCCGCATCAAAGTCATCTCCATGGGCA ACGCCGAAGTGGGGAAAAGCTGTATTATAAAGCGCTACTGTGAGAAGAGATTTGTGTCTAAATACCTGGC AACCATCGGGATTGACTATGGAGTCACAAAGGTCCAAGTCAGAGACAGAGAGATCAAAGTTAACATCTTT GACATGGCTGGACATCCCTTCTTCTTTGAGGTCCGAAATGAGTTTTACAAGGACACACAAGGTGTGATAC TAGTCTATGATGTTGGACAGAAAGACTCATTTGATGCCCTTGATTCGTGGCTGGCAGAGATGAAGCAGGA GCTTGGGCCTCACGGGAACATGGACAACATTGTCTTTGTTGTTTGTGCCAACAAGATCGACTGCTCCAAA CACCGCTGCATCGATGAAAGTGAGGGGCGTCTCTGGGCTGAAAGCAAAGGGTTCCTGTACTTTGAAACAT CAGCACAAACTGGAGAGGGAATCAACGAGATGTTCCAGACCTTTTACTTGTCCATAGTTGACTTGTGTGA AAATGGCGGGAAGCGTCCTACTGCAAGTAGCAGTGCTAGTTTCACCAAAGAACAAGCAGACACTATTCGC AGAATCCGAAATAGTAAGGACAGCTGGGAGATGCTAGGAGTCAGACCTGGAGCCTCAAGGGAGGAAGTTA ATAAGGCCTACCGGAAGCTGGCTGTGCTGCTTCACCCGGACAAGTGTGTAGCACCAGGCAGTGAAGATGC CTTCAAAGCAGTTGTGAATGCCCGGACAGCCCTCCTGAAAAACATCAAG ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA >MG203647 representing NM_153082
Red=Cloning site Green=Tags(s) METNVPKRKEPAKSLRIKVISMGNAEVGKSCIIKRYCEKRFVSKYLATIGIDYGVTKVQVRDREIKVNIF DMAGHPFFFEVRNEFYKDTQGVILVYDVGQKDSFDALDSWLAEMKQELGPHGNMDNIVFVVCANKIDCSK HRCIDESEGRLWAESKGFLYFETSAQTGEGINEMFQTFYLSIVDLCENGGKRPTASSSASFTKEQADTIR RIRNSKDSWEMLGVRPGASREEVNKAYRKLAVLLHPDKCVAPGSEDAFKAVVNARTALLKNIK TRTRPLE - GFP Tag - V |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_153082 |
ORF Size | 819 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_153082.4, NP_694722.2 |
RefSeq Size | 4696 bp |
RefSeq ORF | 822 bp |
Locus ID | 217378 |
UniProt ID | Q8CFP6 |
Gene Summary | GTPase which can activate the MEK/ERK pathway and induce cell transformation when overexpressed. May act as a nuclear scaffold for MAPK1, probably by association with MAPK1 nuclear export signal leading to enhanced ERK1/ERK2 signaling.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC207879 | Dnajc27 (untagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 27 (Dnajc27), (10ug) |
CNY 3,990.00 |
|
MR203647 | Dnajc27 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 27 (Dnajc27) |
CNY 2,400.00 |
|
MR203647L3 | Lenti ORF clone of Dnajc27 (Myc-DDK-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 27 (Dnajc27) |
CNY 4,750.00 |
|
MR203647L4 | Lenti ORF clone of Dnajc27 (mGFP-tagged) - Mouse DnaJ (Hsp40) homolog, subfamily C, member 27 (Dnajc27) |
CNY 4,750.00 |
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